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Method for breaking walls of cells of bran by wet grinding

A wet crushing and cell wall technology, applied in plant cells and other directions, can solve the problems of insufficient cytoplasmic dissolution, low cell wall breaking rate and high temperature rise, and achieve the effect of eliminating cellulose crystal structure, improving enzymatic hydrolysis efficiency and simple method.

Inactive Publication Date: 2012-08-15
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ultrafine pulverization technology in the prior art mainly uses high-speed airflow pulverization and grinding to break the cell wall, but the disadvantages of this pulverization method are high energy consumption and low efficiency; the pulverization process has a high temperature rise and has a great impact on the active ingredients ; The cell wall breaking rate is low, and the cytoplasmic dissolution is not sufficient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 100 g of wheat bran (purchased from Hengxiang Flour Factory in Jurong City) in an agate jar, add water twice the mass of the material, add 2 u / g of cellulase, and put it in a planetary ball mill (XQM-20 L, Nanjing Science and Technology Co., Ltd. The milling speed was 500 rpm, and the temperature was controlled not to exceed 50°C during the pulverization process. After pulverization for 5 hours, the wheat bran and agate balls were separated without drying to obtain the wheat bran wall-broken powder. The measured median diameter was 4.75 μm, and the cell wall breaking rate was 99.2%.

Embodiment 2

[0024] Take 100 g of wheat bran (purchased from Hengxiang Flour Factory in Jurong City) in an agate jar, add water 5 times the mass of the material, add hemicellulase 10 u / g, and put it in a planetary ball mill (XQM-20 L, Nanjing Keyan Experimental Instrument Research Institute) medium ball milling, the ball milling speed is 425 rpm, the temperature is controlled not to exceed 50°C during the pulverization process, after pulverization for 3 hours, the wheat bran and agate balls are separated without drying, and the wheat bran broken wall powder is obtained . The measured median diameter was 3.55 μm, and the cell wall breaking rate was 99.2%.

Embodiment 3

[0026] Take 100 g of wheat bran (purchased from Hengxiang Flour Factory in Jurong City) in an agate jar, add water 9 times the mass of the material, add a compound enzyme mixed with cellulose and hemicellulase, cellulase and hemicellulase The amount of each was 10 u / g, and they were ball-milled in a planetary ball mill (XQM-20 L, Nanjing Keyan Experimental Instrument Research Institute), the milling speed was 350 rpm, the temperature was controlled not to exceed 50°C during the pulverization process, and after 1 h of pulverization, , after the wheat bran is separated from the agate balls, no drying is required to prepare the wheat bran wall breaking powder. The median diameter was 3.05 μm, and the cell wall breaking rate was 99.7%.

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Abstract

The invention relates to a method for breaking walls of cells of bran by wet grinding, and belongs to the technical field of industrial biochemistry. The invention adopts the technical key point that: bran, water, and enzyme for degrading walls of cells of the bran are mixed, and subjected to wet ball-milling grinding to form superfine wall-breaking powder. By combining an ultramicro-pulverization method, the water and the enzyme for degrading the walls of cells of the bran, the cells of the bran are subjected to wet ball milling for wall-breaking treatment; compared with single ultramicro pulverization, the method can greatly reduce the energy consumption, is simple and has low requirement on operating equipment; and compared with single enzymatic degradation, the method combines wet ball milling, combines chemical action with mechanical force action, and has the advantages of greatly saving energy required by high temperature treatment, avoiding the defect of enzyme inactivation under high temperature treatment, eliminating crystal structures of cellulose and improving enzymolysis efficiency.

Description

Technical field [0001] The present invention involves a method of wetting the wall of bran cells, which belongs to the technology of industrial biochemical industry. Background technique [0002] Bran contains natural plant estrogen such as wood, heterofensives, etc. This type of natural plant estrogen has cancer suppressing and reducing cholesterol, and plant estrogen in bran can "reuse" through intestinal bacteria to make the plant plantThe total amount of estrogen can be increased by about 10 times.Therefore, the research of deep -seated bran processing and plant estrogen is of great significance.However, the nutritional components in bran are mainly concentrated in bran cell walls, while bran cell walls are highly fibrosis and very tough. Therefore, if you want to use the above -mentioned nutrients of bran, you must first solve the structure of fully decomposing bran cell walls. [0003] The main components of plant cell walls are cellulose, semi -cellulose, and pectin. Cello...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 吴琴燕杨敬辉庄义庆
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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