Elisa detection method of microbial transglutaminase in frozen surimi

Abstract

The invention relates to an ELISA (enzyme-linked immunosorbent assay) detection method for microbial transglutaminase (MTG) in frozen surimi. The method is as follows: using anti-MTG rabbit polyclonal antibody as a coating antibody and anti-MTG rabbit monoclonal antibody as a detection antibody for combining with HRP (horse radish peroxidase) labeled anti IgG1 second antibody to form the double antibody sandwich ELISA detection method for quantitatively detecting that whether the frozen surimi is added with the MTG. The detection limit of the method is 20ng / mL, in the range of 0.6mg / mL to 10mg / mL, the relationship between OD450 value and log value of MTG concentration is linear, frozen surimi sample adding recovery rate is over 94%, the in-plate variation coefficient is 1.12% ~ 4.02%, and the between-plate variation coefficient is 5.43% ~ 6.87%. The method is high in sensitivity, simple in sample pretreatment, and suitable for the quantitative detection of the MTG in the frozen surimi, and can prevent raw material adulteration of the frozen surimi.

Description

technical field [0001] The invention relates to an ELISA detection method for microbial transglutaminase in frozen surimi, belonging to the technical field of food safety detection. Background technique [0002] With the continuous improvement of living standards and the accelerated pace of life, people are more and more fond of processed aquatic foods that are safe, hygienic, nutritious, and convenient, which promotes the vigorous development of the frozen surimi and its products processing industry. At present, the raw materials for producing frozen surimi are mainly concentrated in seawater fish such as Alaska pollock and Pacific cod. However, due to the excessive fishing intensity and environmental pollution in the past, the main marine economic fish have been seriously depleted in recent years, resulting in an increase in the production cost of seawater surimi. Some unscrupulous traders add microbial transglutaminase (MTG) in the process of making frozen surimi to enha...

AI Technical Summary

Problems solved by technology

In order to regulate the market of surimi raw materials and prevent adulterated raw materials from causing quality deterioration in the processing of surimi products, it is imminent to establish a reliable MTG detection method

At present, domestic research on MTG detection is limited to the detection of MTG enzyme activity. For surimi containing endogenous TGase, enzyme activity detection cannot determine whether MTG is added to frozen surimi

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