Agamogony breeding method of porphyra yezoensis

Abstract

The invention relates to an agamogony breeding method of porphyra yezoensis. The agamogony breeding method comprises the following steps: selecting single plant porphyra yezoensis thallus, of which the surface is washed, dried in the shade, and frozen, of a required character expression according to a breeding aim; taking out, and washing the frozen thallus again; separating the frozen thallus for cutting microchip tissue; carrying out static cultivation for the microchip tissue in a PES culture solution or the culture solution of adding N and P into boiled seawater under the condition of weak light and 15 to 18 degrees centigrade to acquire germplasm mitoplast. According to the agamogony breeding method, the germplasm mitoplast is formed by the object tissue in a way of agamogony but not gamogenesis; besides, the germplasm mitoplast can be stored as a provenance for years, and can be multiplied for using anytime; the time for purifying and breeding germplasm repeatedly in a conventional porphyra breeding process is made up and shortened, and the condition and the possibility are created for acquiring more porphyra yezoensis pure line germplasm provided with excellent characters.

Description

technical field [0001] The invention belongs to the field of laver breeding, in particular to a method for apogamous breeding of Porphyra zebra. Background technique [0002] Porphyra thallus is the haploid generation in its life history, which cannot be preserved as seedlings, while the diploid generation in the filamentous stage can be preserved as seedlings for a long time. Porphyra zebra fronds are hermaphroditic plants, which belong to the type of mixed male and female germ cells, and the self-fertilization rate reaches 50%. The fruit spores are the products of fertilization, and the filaments germinate from them. For this reason, traditional production uses fruit spores to collect seedlings and cultivate seedlings. There is uncertainty in the genetic background of the provenance, and the homogeneity of the population cannot be avoided, and the quality of germplasm traits declines rapidly. Judging from the progress of laver breeding research at home and abroad, selecti...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for apogamous breeding of Porphyra zebra, which overcomes the time-consuming problem in the process of breeding improved varieties of Porphyra variegata in the past