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Apparatus for rapidly detecting allergic peanut proteins in food, and making method thereof

A technology for allergenic proteins and peanuts, which is applied in the field of rapid detection of peanut allergenic proteins in food and its preparation field, can solve the problems of low contrast between band and background, large background interference, and difficulty in realizing multiple detection and combination, and achieve rapid Accurate qualitative and quantitative detection, easy manufacture, and long service life

Inactive Publication Date: 2014-10-15
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) The colloidal gold labeling process is an electrostatic adsorption process, which is a physical adsorption, so it has poor stability in the liquid phase.
[0008] Often cause the labeled protein molecules to fall off again
[0009] (2) The result is judged by displaying a single purple-red strip, which is single in color, and it is difficult to achieve multiple detection and joint detection.
[0012] The bands have little contrast to the background, limiting detection sensitivity
[0013] (4) The matrix effect of different materials is obvious, and the background interference is very large
[0014] (5) The detection sensitivity is low
[0015] (6) Unable to achieve accurate quantitative detection
[0019] However, it is still difficult to meet the requirements of sensitivity and detection stability for the quantitative detection of peanut allergenic protein by conventional fluorescent microsphere immunochromatography method

Method used

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  • Apparatus for rapidly detecting allergic peanut proteins in food, and making method thereof
  • Apparatus for rapidly detecting allergic peanut proteins in food, and making method thereof
  • Apparatus for rapidly detecting allergic peanut proteins in food, and making method thereof

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Embodiment 1

[0054] 1. Assembly of fluorescent labeling device;

[0055] 1. Preparation of NC membrane:

[0056] Preparation of detection area and quality control area: Peanut allergenic protein polyclonal antibody and anti-rabbit antibody coated on nitrocellulose membrane: use 0.1 M pH 7.2 PBS (phosphate buffer saline, which contains 5% sucrose and 0.05% Tween-20 (Tween-20)) adjusted the concentration of the peanut allergenic protein polyclonal antibody to 0.5 mg / mL, and the resulting solution was sprayed on the membrane as the detection area, and 0.01 M PBS pH 7.2 (which contained 5% sucrose and 0.05 % Tween-20 (Tween-20)) to adjust the concentration of anti-rabbit antibody to 0.5 mg / mL, and the resulting solution was sprayed on the membrane as a quality control area, and the spray volume of the two areas was 0.74 μL / cm, The distance between the two areas is 5 mm, and the quality control area is 2 mm away from one end of the nitrocellulose membrane. After drying at 37°C overnight, store...

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Abstract

The invention discloses an apparatus for rapidly, conveniently, efficiently and quantitatively detecting allergic peanut proteins in a food, and a making method thereof, and belongs to a fluorescence microsphere labeled detection technology. The apparatus comprises a special property labeled biological macromolecule with biological specificity and a polymeric material film with adsorption mobility as carriers, and detection is carried out in the portable and small square apparatus. The special property of a tag is used to quantitatively detect a trace amount of allergic peanut proteins contained in the matrix of the food, and the detection limit reaches 0.1ng / mL. The special tag overcomes the problems of instability and low detection value of common tags. The apparatus is mainly used in the quantitative detection of residual peanut allergens in food safety.

Description

technical field [0001] The invention belongs to the field of detection of peanut allergenic protein in food in food safety, and specifically relates to a fluorescent microsphere labeling device for qualitatively and quantitatively quickly detecting peanut allergenic protein in food and a preparation method thereof. Background technique [0002] In recent years, with the improvement of people's living standards, food safety issues have become more and more prominent, including food allergies. Food allergy refers to an allergic reaction of the body to a certain substance or component in food. It is a hypersensitivity reaction mediated by IgE and non-IgE, which can cause reactions in the skin, gastrointestinal tract, and respiratory system. In severe cases, it may even lead to systemic allergic reactions or shock. [0003] Peanut allergy is an IgE-mediated hypersensitivity reaction, and its mechanism is mainly type I hypersensitivity reaction mechanism. A certain amount of all...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/68G01N33/558
Inventor 赖卫华倪小琴刘道峰山珊彭涛
Owner NANCHANG UNIV
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