Apparatus for rapidly detecting allergic peanut proteins in food, and making method thereof

A technology for allergenic proteins and peanuts, which is applied in the field of rapid detection of peanut allergenic proteins in food and its preparation field, can solve the problems of low contrast between band and background, large background interference, and difficulty in realizing multiple detection and combination, and achieve rapid Accurate qualitative and quantitative detection, easy manufacture, and long service life
CN104101581AInactive Publication Date: 2014-10-15NANCHANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANCHANG UNIV
Publication Date
2014-10-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an apparatus for rapidly, conveniently, efficiently and quantitatively detecting allergic peanut proteins in a food, and a making method thereof, and belongs to a fluorescence microsphere labeled detection technology. The apparatus comprises a special property labeled biological macromolecule with biological specificity and a polymeric material film with adsorption mobility as carriers, and detection is carried out in the portable and small square apparatus. The special property of a tag is used to quantitatively detect a trace amount of allergic peanut proteins contained in the matrix of the food, and the detection limit reaches 0.1ng / mL. The special tag overcomes the problems of instability and low detection value of common tags. The apparatus is mainly used in the quantitative detection of residual peanut allergens in food safety.
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Description

technical field

[0001] The invention belongs to the field of detection of peanut allergenic protein in food in food safety, and specifically relates to a fluorescent microsphere labeling device for qualitatively and quantitatively quickly detecting peanut allergenic protein in food and a preparation method thereof. Background technique

[0002] In recent years, with the improvement of people's living standards, food safety issues have become more and more prominent, including food allergies. Food allergy refers to an allergic reaction of the body to a certain substance or component in food. It is a hypersensitivity reaction mediated by IgE and non-IgE, which can cause reactions in the skin, gastrointestinal tract, and respiratory system. In severe cases, it may even lead to systemic allergic reactions or shock.

[0003] Peanut allergy is an IgE-mediated hypersensitivity reaction, and its mechanism is mainly type I hypersensitivity reaction mechanism. A certain amount of all...

Claims

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