Method for rapidly releasing dormancy of brucea javanica seeds

A technology of bruce japonica and seeds, which is applied in the directions of seed and rhizome treatment, application, agriculture, etc., can solve the problems of different seed maturity, inconsistent emergence, and long breeding cycle of seedlings, so as to shorten the breeding cycle, and achieve neat and consistent seedlings. , the effect of improving the germination rate

Active Publication Date: 2014-10-22
海南万玮制药有限公司
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AI Technical Summary

Problems solved by technology

The huge demand for Brucea javanica as a raw material for medicine has led to serious damage to wild resources, and the collection of wild resources can no longer meet production needs. Standardized production is an effective way to solve the shortage of medicinal materials, and seedling breeding is an important part of standardized production. Brucea javanica fruit has a long ripening cycle, different degrees of seed maturity, plus the difficulty of separating the pulp from the seeds, the seeds are perishable, and the seed coat is hard, etc., resulting in a long breeding cycle for the seedlings of Brucea javanica and inconsistent emergence, which seriously affects the growth of Brucea javanica. Standardized cultivation
[0003] Through the retrieval of domestic and foreign literature and patents, a total of 1 literature was retrieved on the germination of Brucea javanica seeds and seedling propagation, Li Xianghong et al. Chinese Horticultural Abstracts, 2009 (1): 42-45.) briefly introduced the germination technology of Brucea javanica seeds, and the result is that: Brucea javanica seeds need to be placed for a period of time to pass through the dormant period, and then heated at 50~55°C Soak the seeds in water for 15-20 minutes, wash them for 2-3 times, then soak them in water for 12-24 hours, and place them in an environment of 33-35°C for germination. During the germination process, keep the humidity. After 7-10 days, the seeds will germinate one after another. 10% to 20% of the bud points can be sown, but the germination of Brucea javanica is irregular, and the emergence of seedlings lasts for a year

Method used

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  • Method for rapidly releasing dormancy of brucea javanica seeds
  • Method for rapidly releasing dormancy of brucea javanica seeds
  • Method for rapidly releasing dormancy of brucea javanica seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] experimental method

[0027] The fruits of Brucea javanica were divided into two groups, a blank control group and a group stored at 4°C for 3 days, 6 days, 10 days and 14 days, respectively. Then sterilized with 1% by weight sodium hypochlorite aqueous solution for 15 minutes, rinsed with sterile water 5 times until pH = 7, paralleled four groups for each concentration, and placed 100 grains in each group for full light cultivation in a constant temperature incubator at 30°C, and counted the germination rate every 10 days .

[0028] Table 1 Effect of low temperature storage on germination of Brucea javanica seeds

[0029]

[0030] Experimental results

[0031] As shown in Table 1, when the seeds of Brucea javanica were not stored at low temperature, they began to germinate on the 20th day, but the germination rate was low, and the germination rate was lower than 5.0%; The germination rate can be as high as 1.1%. It reached the peak at 50 days, and about 31.0% of ...

Embodiment 2

[0033] experimental method

[0034] The fruits of Brucea javanica were divided into two groups, one group was not depulped with fine sand (blank control), and the other group was immersed in warm water at 20°C to 30°C for 1 day, and rubbed with 5% by weight of fine sand to remove the pulp. Then disinfect with 1% by weight sodium hypochlorite aqueous solution for 15 minutes, rinse with sterile water 5 times until pH=7, parallel four groups for each concentration, and place 100 grains in each group for full light cultivation in a constant temperature incubator at 30°C, and count germination every 10 days Rate.

[0035] Table 2 The effect of removing the pulp or not on the germination of Brucea javanica seeds

[0036]

[0037] Experimental results

[0038] As shown in Table 2, when the fruit of Brucea javanica is not removed, it will start to germinate on the 10th day, but the germination rate is low, and the germination rate is lower than 5.0%; it reaches the peak at 50 day...

Embodiment 3

[0040] experimental method

[0041] The seeds of Brucea javanica were divided into 6 groups, wherein the first group was a blank control; the second group was soaking the fruit of Brucea javanica in warm water at 20°C to 30°C for 1d, and rubbing it with 5% by weight of fine sand to remove the pulp; The first group is to immerse the fruit of Brucea javanica in warm water at 20°C to 30°C for 3 days, and rub it with 5% by weight of fine sand to remove the pulp; the fourth group is to soak the fruit of Brucea javanica in warm water at 20°C to 30°C for 3 days, and rub it with % fine sand is rubbed, to remove the pulp; the 5th group is to immerse the fruit of Brucea javanica in 20 ℃~30 ℃ of warm water for 3d, and rub with the fine sand of 20% by weight, to remove the pulp; the 6th group is to use the fruit of Brucea javanica 20 Soak in warm water at ℃~30℃ for 5 days, and rub with 20% by weight of fine sand to remove the pulp. Then sterilized with 1% by weight sodium hypochlorite aq...

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Abstract

The invention relates to a method for rapidly releasing dormancy of brucea javanica seeds, which comprises the following steps: 1)storing ripe brucea javanica fruits for 7-14 days at low temperature of 4 DEG C; 2)immersing brucea javanica fruits for 1-5 days in warm water at 20-30 DEG C, kneading by using 5wt%-20wt% of fine sand and removing pulp; 3)processing brucea javanica seeds by concentrated sulfuric acid with concentration of 98% for 30-90 seconds, and using running water for flushing until no sulfuric acid is residual; 4)processing brucea javanica seeds by using 100-300mg/L of gibberellin (GA3) aqueous solution for 2-48 hours; and 5) sprouting the processed brucea javanica seeds. The method has the advantages of shortening of seedling growing period and tidiness of seedling, and can be widely used for seedling, breeding and cultivating of medicinal material brucea javanica seeds.

Description

technical field [0001] The invention relates to the field of germination of medicinal plant seeds, in particular, the invention relates to a method for quickly releasing the dormancy of the seeds of Brucea javanica. Background technique [0002] Brucea javanica (L.) Merr.Rhusjavanica L. is the dried fruit of Brucea javanica (L.) Merr. Rhusjavanica L., which is the best-selling Chinese patent medicine such as Brucea javanica oil soft capsule, Brucea javanica oil oral emulsion, Brucea javanica oil emulsion injection, etc. raw material. In recent years, Chinese patent medicines with Brucea javanica as functional raw materials have remarkable curative effects, and the demand has grown rapidly, which has caused the price of raw materials of Brucea javanica to rise year after year. The supply of raw materials is seriously insufficient, and the shortage of goods is serious. The huge demand for Brucea javanica as a raw material for medicine has led to serious damage to wild resourc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
Inventor 姚俊华庞玉新于福来张影波陈振夏
Owner 海南万玮制药有限公司
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