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Method for rapid release of Callicarpa nudiflora seed dormancy

A naked flower purple bead and seed technology, applied in the direction of seed and rhizome treatment, application, agriculture, etc., can solve the problems of inconsistent emergence, different seed maturity, hard seed coat, etc., achieve uniform seedlings, and shorten the breeding cycle. , the effect of improving the germination rate

Active Publication Date: 2015-01-14
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The huge demand for the naked flower purple pearl as a medical raw material has led to serious damage to wild resources, and the collection of wild resources can no longer meet the production needs. Standardized production is an effective way to solve the shortage of medicinal materials, and seedling breeding is an important part of standardized production , due to the long ripening period of the nude flower fruit, the different maturity of the seeds, the small size of the seeds, the hard seed coat, etc., the long breeding cycle of the nudibranch seedlings and the inconsistent emergence have seriously affected the nude flower standardized cultivation of

Method used

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  • Method for rapid release of Callicarpa nudiflora seed dormancy
  • Method for rapid release of Callicarpa nudiflora seed dormancy
  • Method for rapid release of Callicarpa nudiflora seed dormancy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] experimental method

[0032] Divide the seeds of Aurora nudiflora into two groups, one group is soaked with 0.1% hydrochloric acid (that is, 0.1 volume % hydrochloric acid aqueous solution) for a certain period of time, and the other group is blank. Then disinfect with 1% by weight sodium hypochlorite aqueous solution for 15 minutes, rinse with sterile water 5 times until pH=7. Four groups were paralleled for each concentration, and 100 grains in each group were cultured in full light in a constant temperature incubator at 30°C, and the germination rate was counted every day.

[0033] Table 10.1% The effect of hydrochloric acid treatment on the seed germination of Aurora nudiflora

[0034]

[0035] Experimental results

[0036]As shown in Table 1, when the nudibranch seeds were not treated with 0.1% hydrochloric acid, they began to germinate on the 10th day, but the germination rate was low, and the germination rate was lower than 5.0%; During the period, the dail...

Embodiment 2

[0038] experimental method

[0039] Divide the seeds of A. nudiflora into two groups, one group was soaked with different concentrations (W / W) of hydrogen peroxide for 2 hours, and the other group was blank. Then disinfect with 1% by weight sodium hypochlorite aqueous solution for 15 minutes, rinse with sterile water 5 times until pH=7. Four groups were paralleled for each concentration, and 100 grains in each group were cultured in full light in a constant temperature incubator at 30°C, and the germination rate was counted every 5 days.

[0040] Table 2 Effect of hydrogen peroxide treatment on seed germination

[0041]

[0042] Experimental results

[0043] As shown in Table 2, when the seeds of Aurora nudiflora were not treated with hydrogen peroxide, they began to germinate on the 10th day, but the germination rate was low, and the germination rate was lower than 5.0%; when there was a germination concentration period at about 20 days, The daily average germination ra...

Embodiment 3

[0045] experimental method

[0046] Divide the seeds of P. nudiflora into two groups, one group was soaked with different concentrations of plant ash aqueous solution for 24 hours, and the other group was blank. Then disinfect with 1% by weight sodium hypochlorite aqueous solution for 15 minutes, rinse with sterile water 5 times until pH=7. Four groups were paralleled for each concentration, and 100 grains in each group were cultured in full light in a constant temperature incubator at 30°C, and the germination rate was counted every 5 days.

[0047] Table 3 The effect of plant ash treatment on the seed germination of P. nudiflora

[0048]

[0049] Experimental results

[0050] As shown in Table 3, when the seeds of the nudibranch are not treated with the plant ash aqueous solution, they will start to germinate on the 10th day, but the germination rate is low, and the germination rate is lower than 5.0%; there will be a germination concentration period at about 20 days ,...

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Abstract

The invention relates to a method for rapid release of Callicarpa nudiflora seed dormancy. The method comprises the following steps: 1) impregnating Callicarpa nudiflora seeds for 12-24h with one or more selected from 5-20 wt% of a plant ash water solution, 100-300mg / L agibberellin (GA3) aqueous solution and 100-300mg / L alpha-naphthaleneacetic acid (NAA), and then washing the seedswith water; and step 2) germinating the impregnated seeds in the step 1). The method for rapid release of Callicarpa nudiflora seed dormancy provided by the invention can effectively relieve various factors hindering germination of the Callicarpa nudiflora seeds, including mechanical barrier formed by the seed coat, break Callicarpa nudiflora seed dormancy, and enhance seed germination rate to 95%. Compared with a traditional seedling raising way without treatment, the method has advantages of shortened breeding cycle, uniform seedlings, and simple operation and low cost, and can be widely used for breeding and cultivation of a Callicarpa nudiflora medicinal material.

Description

technical field [0001] The invention relates to the field of germination of medicinal plant seeds, in particular, the invention relates to a method for quickly releasing the dormancy of the seeds of the nudibranch. Background technique [0002] Callecarpa nudiflora Hook.et Arn is the dry leaf of Callecarpa nudiflora Hook.et Arn, which is recorded in the 1977 edition of Chinese Pharmacopoeia. It has the functions of stopping bleeding, dispelling blood stasis, antibacterial and anti-inflammation, etc. It is an important ingredient of more than 40 kinds of Chinese patent medicines, such as nudehuazizhu tablet, nudehuazizhu dispersible tablet, nudehuazizhu capsule, nudehuazizhu granule, nudehuazizhu suppository, etc. raw material. The huge demand for the naked flower purple pearl as a medical raw material has led to serious damage to wild resources, and the collection of wild resources can no longer meet the production needs. Standardized production is an effective way to solve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00
Inventor 王丹张影波庞玉新于福来官玲亮张新蕊胡熊飞
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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