Preparation method and application of recombinant canine interleukin-2
An interleukin and recombinant protein technology, which is applied in the field of the preparation of recombinant canine interleukin-2, can solve the problems of little research on recombinant protein and no eukaryotic expression, and achieves high production efficiency and promotes canine lymphocyte proliferation. , the effect of low production cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0044] 1. Amplification of canine interleukin-2 mature protein gene
[0045] (1) Isolation and activation of canine peripheral blood lymphocytes
[0046] Aseptically collect peripheral venous blood from dogs, separate lymphocytes with lymphocyte separation medium, wash with PBS two to three times, centrifuge, and culture in 1640 nutrient solution containing 10% calf serum to make the cell density 5×10 6 / ml, then add ConA to make the final concentration 20 μg / mL, in 5% CO 2 , Cultivate at 37°C for 18 h.
[0047] (2) Extraction of total cellular RNA
[0048]The cells were divided into 1.5 mL centrifuge tubes, and the supernatant was discarded after centrifugation at 3000 g for 5 min. One-step extraction of total RNA from lymphocytes with Trizol kit: Add 200 μL Trizol Reagent and shake vigorously. Stand at room temperature for 5 min. Then add 40 μL of chloroform, and vigorously invert the centrifuge tube to mix. After standing for 5 min, centrifuge at 12000 g for 15 min at...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com