A kind of tissue culture method and culture medium of precocious pear stem section

A technology of tissue culture and inoculation medium, applied in the field of plant cell molecular biology, can solve the problems of average rooting rate, low average root number, low proliferation coefficient of test-tube plantlets, low reproduction coefficient, etc., to reduce browning rate, shorten Growth time, effect of increasing proliferation coefficient

Active Publication Date: 2016-08-24
JIANGSU ACAD OF AGRI SCI
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  • Summary
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AI Technical Summary

Problems solved by technology

[0004] When Sucui 1 and Sucui 2 were propagated by conventional cutting methods, the reproduction coefficients were low and highly heterozygous; when conventional tissue culture methods were used for propagation, the proliferation coefficient of test-tube plantlets was low, and the average rooting rate and average root number were lower than those of Sucui 2. low, shorter root length

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Tissue culture of pear stem segments of 'Su Cui No. 1' and 'Su Cui No. 2' was carried out as follows.

[0049] Collection and disinfection of explants: From October to December of the previous year, annual shoots were collected from the orchard and stored in a 4°C refrigerator. From late February to early March of that year, the branches were taken and inserted into sterile water, at 22°C-25°C, 8 hours of light per day, 16 hours of darkness, light intensity of 1500-2000lx, germination was accelerated, and fresh sterile water was changed every 7 days. In early and mid-April, after the buds germinate, take multiple stem sections with buds, cut them into 1-1.5cm high stem sections with one bud, and perform the following disinfection treatment: use 100mg / L carbenzyl carboxybenzyl After soaking in tap water with penicillin for 2.5 hours, vortex for 5-8 minutes with a mixed disinfectant solution, then peel off the bracts, and perform secondary disinfection with a mixed disinf...

Embodiment 2

[0081] Example 2 Adopt conventional tissue culture rapid propagation method to cultivate 'Su Cui No. 1' and 'Su Cui No. 2'

[0082] The following methods were used to cultivate 'Su Cui No. 1' and 'Su Cui No. 2':

[0083] 1) Collection and disinfection of explants: Select robust annual branches from the field, cut young branches as explants, rinse with washing powder, and rinse with tap water for 30 minutes. On the ultra-clean workbench, the treated explants were sterilized with 75% alcohol for 20 seconds, 0.1% mercuric chloride for 8-10 minutes, and rinsed with sterile water for 4-5 times. The scales were removed with a scalpel, and the naked buds were cut and inoculated on the induction medium, and 3 explants were inoculated in each bottle. The induction medium is: add 6-BA, IBA, GA to 1 / 2MS medium 3 , sucrose and agar, wherein the final concentration of 6-BA is 1.0mg / L, the final concentration of IBA is 0.2mg / L, GA 3 The final concentration of sucrose is 2.0mg / L, the fina...

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Abstract

The invention provides a tissue culture method and culture medium for early-maturing pear stem segments, which belong to the technical field of plant cell molecular biology. The tissue culture method comprises the following steps: using the initial inoculation medium to induce differentiation and culture of the explants to obtain the first-generation tissue culture plantlets; using the proliferation medium to perform proliferation and culture of the first-generation tissue culture plantlets, and then cutting out new slightly transformed plantlets. Insert the strong seedling medium for subculture, and then transfer to rooting medium A and rooting medium B in turn for rooting culture. The medium used for tissue culture of early-maturing pear stem segments is composed of one or more combinations of initial inoculation medium, proliferation medium, strong seedling medium, and rooting medium. The tissue culture method of the invention has the advantages of safe disinfection method and short time, and significantly improves the proliferation coefficient, rooting rate, average root length and average root number of tissue culture seedlings.

Description

technical field [0001] The invention belongs to the field of plant cell molecular biology, and in particular relates to a tissue culture method and culture medium for pear stem segments. Background technique [0002] Sucui No. 1 is a high-quality early-maturing sand pear. The parent is Huasu × Cuiguan. It was crossed in 2003, and the hybrid seedlings were cultivated in 2004. In 2011, it was approved by the Jiangsu Provincial Crop Variety Approval Committee (Su Jianguo 201107). The pulp is white, fine and crisp, with few or no stone cells, juicy and sweet, with good flavor and good quality. It is easy to form flower buds, has strong continuous fruiting ability, high fruit setting rate, and the fruit ripening period is early July. [0003] Sucui No. 2 is also an early-maturing sand pear. The parent is Xizilu × Cuiguan. It was crossed in 2003, and the hybrid seedlings were cultivated in 2004. It passed the variety approval of Jiangsu Province in 2011. The fruit is obovate, wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王宏蔺经常有宏马娜甘慧芳
Owner JIANGSU ACAD OF AGRI SCI
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