Method for identifying female Parasarcophaga similis by using uterus spicule
A flax fly and bone chip technology, applied in the field of insect identification, can solve problems such as inability to obtain accurate identification, and achieve the effects of high accuracy, simple operation and short duration
Active Publication Date: 2014-11-05
岳巧云
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Problems solved by technology
However, the specimens intercepted during the quarantine process are often females and cannot usually be accurately identified
Method used
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Embodiment 1
[0023] (1) The mating male and female flies are collected in the wild;
[0024] (2) Use the tail organs and external characteristics of the males to identify the males, so as to determine the species of the paired female flies.
[0025] (3) Using DNA barcoding technology, compare whether the DNA barcode sequences of the paired male and female flies are consistent, so as to determine whether the female flies are consistent with the paired male species. The specific steps are as follows: take a leg of the sargassum fly, soak it in the lysate in the animal tissue genomic DNA extraction kit (TIANGEN; code: DP304) for more than 5 hours, and extract the genomic DNA with the animal tissue genomic DNA extraction kit. Using LCO1490 and HCO2198 as primers, by PCR, the reaction system is: 5 times Phusion HF buffer 10μl, 10mM dNTPs 1μl, forward and reverse primers (concentration: 10pmol / L) each 1μl, Phusion ultra-fidelity DNA polymerase 0.5μl, genome 2 μl of DNA, 50 μl supplemented with ...
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The invention discloses a method for identifying female Parasarcophaga similis by using a uterus spicule. The shape of the uterus spicule of female Parasarcophaga similis likes an irregular vase plane with a narrow top and a wide bottom, and camber lines at two sides inward extends to form a neck structure, then outward extends and finally inward contracts to form an abdomen structure from the narrow end to the wide end. The method comprises the following steps: a specimen to be checked is dissected to obtain the uterus spicule; the uterus spicule of the specimen to be checked is compared with the uterus spicule of female Parasarcophaga similis, and the specimen to be checked is the female Parasarcophaga similis if shapes are basically same; and the specimen to be checked is not the female Parasarcophaga similis if the shapes are different. The inventors of the invention find that the uterus spicule has species specificity, and can be used for identifying whether the specimen to be checked is the female Parasarcophaga similis or not. The method for identifying female Parasarcophaga similis by using uterus spicule has the advantages of simplicity, short time and high accuracy, and is especially suitable for the application in the forensic identification field and / or the inspection and quarantine field.
Description
technical field [0001] The invention belongs to the field of insect identification, in particular to a method for identifying female flax flies by using uterine bone slices. Background technique [0002] There are many kinds of hemp flies, which are distributed all over the world. There are about 2,500 known species in the world. They can carry a variety of pathogens and are one of the important vector organisms. [0003] Accurate identification of the species of sargassum is of great significance for further taking appropriate epidemic prevention measures and preventing the spread of diseases. Since some flies are carnivorous, they are also important forensic insects. According to the developmental state of the flies on the corpse, the time of death and whether it is the first crime scene can be inferred. However, the current identification of the species of Sarbina flies internationally is based on the morphology of the tail organs of male Sarbina flies. Different types o...
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IPC IPC(8): G01N33/48
Inventor 岳巧云汪小东邱德义魏晓雅胡佳陈健刘德星
Owner 岳巧云