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Method for elimination of ascorbic acid interference in clinical examination, reagent and kit

A technology for clinical testing and ascorbic acid, applied in the field of clinical detection reagents, can solve the problems of ascorbic acid interference, chemical oxidation method measurement result interference, poor anti-interference ability of ascorbic acid, etc., and achieves the effect of increasing reliability.

Active Publication Date: 2014-11-05
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ascorbic acid in serum samples is a substance with redox properties, which may interfere with the results of the chemical oxidation method, resulting in abnormal results and affecting the reliability of clinical results
[0004] At present, the reagents on the market that use the chemical oxidation method to detect substrates, such as the kits for the determination of bilirubin by the vanadate oxidation method, generally have poor anti-interference ability to ascorbic acid, which is also a common problem of chemical oxidation reagents. defect
However, so far there is no relevant literature or patent report on how to solve the problem of ascorbic acid interference in the chemical oxidation method determination process

Method used

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  • Method for elimination of ascorbic acid interference in clinical examination, reagent and kit
  • Method for elimination of ascorbic acid interference in clinical examination, reagent and kit
  • Method for elimination of ascorbic acid interference in clinical examination, reagent and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 The elimination ascorbic acid interference test of peroxide

[0051] Formula Table

[0052] Reagent 1 (pH3.0)

[0053]

[0054] The specific dosage of carbamide peroxide (or hydrogen peroxide, or peracetic acid) is shown in Table 1

[0055] h 2 O to 1.0L

[0056] Reagent 2 (pH7.0)

[0057] Sodium dihydrogen phosphate 1.0g

[0058] Sodium metavanadate 0.5g

[0059] h 2 O to 1.0L

[0060] The method for determining direct bilirubin in a sample by using the embodiment of the present invention is as follows: add reagent 1 to the sample (calibration tube as a sample) and mix well, and read the absorbance A1 after incubating at 37°C for 3 to 5 minutes; add reagent 2, mix well , read the absorbance A2 after reacting at 37°C for 5 minutes; according to the formula: direct bilirubin content = measured absorbance (A2-A1) × calibration solution / calibration absorbance (A2-A1) to obtain the direct bilirubin content. Wherein, the measurement wavelength is 4...

Embodiment 2

[0066] Embodiment 2 Elimination of ascorbic acid interference test of persulfate

[0067] Formula Table

[0068] Reagent 1 (pH3.0)

[0069]

[0070]

[0071] The specific dosage of potassium persulfate (or sodium persulfate) is shown in Table 2

[0072] h 2 O to 1.0L

[0073] Reagent 2 (pH7.0)

[0074] Sodium dihydrogen phosphate 1.0g

[0075] Sodium metavanadate 0.5g

[0076] h 2 O to 1.0L

[0077]Take by weighing the material in the formula table, take by weighing persulfate wherein by the amount in table 2, by the preparation method among the embodiment 1, prepare the test kit (prescription 12-17) that contains different kinds and concentration persulfate respectively , Formulation 11 is a persulfate-free kit. The sample and detection method for detection are the same as in Example 1. The results are shown in Table 2. The results show that when using the kit containing the persulfate to detect ascorbic acid samples, the interference deviation between the te...

Embodiment 3

[0080] Embodiment 3 Elimination of ascorbic acid interference test of sulfite

[0081] Formula Table

[0082] Reagent 1 (pH3.0)

[0083]

[0084] The specific dosage of sodium sulfite (or sodium bisulfite) is shown in Table 3

[0085] h 2 O to 1.0L

[0086] Reagent 2 (pH7.0)

[0087] Sodium dihydrogen phosphate 1.0g

[0088] Sodium metavanadate 0.5g

[0089] h 2 O to 1.0L

[0090] Take by weighing the material in the formula table, take by weighing the sulfite wherein in the amount in table 3, by the preparation method in the embodiment 1, prepare the test kit (prescription 19-24) that contains different kinds and concentrations of sulfite respectively , Formula 18 is a sulfite-free kit. The sample and detection method for detection are the same as in Example 1. The results are shown in Table 3. The results show that when using the kit containing the sulfite to detect the ascorbic acid sample, the interference deviation between the test result and the blank sampl...

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Abstract

The invention discloses a clinical examination reagent. The clinical examination reagent can eliminate ascorbic acid interference in chemical oxidation-based clinical sample detection. The clinical examination reagent contains peroxide, persulfate, sulfite, inorganic ions or acid radical ions having oxidability. The invention also discloses a kit containing the clinical examination reagent, a preparation method of the clinical examination reagent, and a method for eliminating ascorbic acid interference in chemical oxidation-based clinical sample detection. The clinical examination reagent can eliminate ascorbic acid interference in clinical detection and improve result accuracy.

Description

technical field [0001] The invention relates to a clinical detection reagent and method, in particular to a detection reagent capable of eliminating ascorbic acid interference, a preparation method, a kit and a method for eliminating ascorbic acid interference in the process of detecting a substrate by a chemical oxidation method. Background technique [0002] Ascorbic acid participates in various reactions in the body, such as participating in redox processes, and plays an important role in biological oxidation and reduction and cellular respiration. There is about 1500mg of ascorbic acid in the metabolically active pool of ascorbic acid in a normal adult, and the highest storage peak can reach 3000mg. [0003] Determination of serum bilirubin is an important routine detection item in clinical medicine, an important indicator for the diagnosis and differential diagnosis of jaundice diseases, and is of great significance for understanding the development of diseases. The co...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/50
Inventor 蔡锦刚杨薇李希文
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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