53results about How to "Does not affect response" patented technology

The invention discloses a kit and a method for precise quantification of next-generation sequencing libraries different in GC content by qPCR (quantitative polymerase chain reaction). The kit for precise quantification of the next-generation sequencing libraries different in GC content by qPCR comprises a plurality of standard samples different in GC content, library dilution liquid, qPCR premixed liquid, primers and ddH2O, wherein nucleotide sequences of the primers are as shown in SEQ ID NO:1 and SEQ ID NO:2. The invention further provides the method of adopting the kit for precise quantification of the next-generation sequencing libraries different in GC content by qPCR. The kit and the method have advantages of precise quantification, convenience in use, wide application range, high selectivity, high sensitivity, high specificity and high stability and provide a guarantee for high-quality high-efficiency sequencing.

The invention relates to a titanium dioxide content measurement method. The titanium dioxide content measurement method comprises the following steps: (1) digesting a polyester sample by a wet-acid soluble decomposition system; (2) reacting a dilute acid mixture of hydrochloric acid and nitric acid with non-titanium metal salt in a sample to obtain a digestion sample liquid; (3) masking metal ions, which interfere with a main reaction, by ascorbic acid, and preparing a dilute solution to be measured with the absorbance value in the range of 0.2 to 0.8 by taking hydrogen peroxide as a color developing agent; (4) measuring the absorbance value A of the dilute solution to be measured, and measuring the blank absorbance value A0; (5) preparing standard solutions of titanium with a series of ladder concentration, measuring the absorbance values of the standard solutions, and then drawing a standard working curve Y=KX by taking the difference values of the measured absorbance values and the blank absorbance value as horizontal coordinates X and taking the titanium content corresponding to the standard solutions of titanium with a series of ladder concentration as vertical coordinates Y, thereby obtaining the slope value K of the standard working curve; and (6) calculating the percentage of titanium dioxide TiO2. By the measurement method disclosed by the invention, the content of titanium dioxide in polyester can be measured accurately.

The invention relates to a single-phase titanium diselenium nano-sheet prepared by a solution method and a method and an application of the nano-sheet. The method includes the steps: dispersing selenium powder in a high-boiling-point solvent to obtain precursor solution A; adding a titanium source into the precursor solution A to obtain mixed solution B; heating the mixed solution B to reach the temperature of 240-300 DEG C under continuous stirring and protective atmosphere, and keeping the temperature for 30min to 90min; cooling mixture to reach indoor temperature after temperature keepingis completed, and separating, washing and drying the mixture to obtain the single-phase titanium diselenium nano-sheet. The molar ratio of the titanium source to the selenium powder is 1:(0.4-3). By aliquid phase method, a high-temperature and time consumption solid phase stage is avoided, complex devices are omitted, the pure hexagonal-phase TiSe2 nano-sheet can be synthesized by keeping the temperature of 240-300 DEG C for 30min-90min according to the conditions such as the molar ratio of the titanium source to the selenium powder of 1: (0.4-3), and the nano-sheet can be used for the fieldof photocatalysis.

The invention provides a new application of an acyl coenzyme A:cholesterol acyltransferase ACAT1 inhibitor. The invention discloses the acyl coenzyme A:cholesterol acyltransferase ACAT1 inhibitor. The acyl coenzyme A:cholesterol acyltransferase ACAT1 inhibitor disclosed by the invention has the advantages that the immune response capacity of CD8 T cells can be enhanced; the killing activity of the CD8 T cells for tumors can be enhanced; the effect function of the CD8 T cells can be enhanced; the multiplication of the CD8 T cells can be promoted; the apoptosis of the CD8 T cells can be reduced; the clustering of T-cell antigen receptors (TCR) on plasma membranes of the CD8 T cells can be promoted; the directional release of cytotoxic particles from the CD8 T cells can be promoted; and the formation of immunological synapses of the CD8 T cells can be promoted. The acyl coenzyme A:cholesterol acyltransferase ACAT1 inhibitor can be used for immunotherapy of the tumors or improving the immunocompetence to the tumors.

The invention discloses a node synchronization method and apparatus for a market supervision blockchain system. An encrypted queue synchronization method is performed among a plurality of blockchain nodes of the market supervision blockchain system, so that a function of synchronizing a plurality of node encryption queues is provided for the market supervision blockchain system; with the additionof the queue synchronization method, the consistency of data in all nodes of the whole market supervision blockchain system is guaranteed, and such a characteristic is not possessed by a mainstream market supervision blockchain system, and thus the overall robustness of the market supervision blockchain system can be effectively improved; when a block system of some node or several nodes in the market supervision blockchain system is collapsed and is damaged, the integrity of file data can also be guaranteed to the greatest extent, and the response of data of the market supervision blockchainsystem to a high-frequency application is not affected; and when any copy is obtained by an illegal intruder, the alteration cannot be performed according to a storage space where a treaty content isfound.

The invention relates to equipment and a method of continuously and rapidly preparing graphene under a normal pressure. The equipment comprises a box, a constant-temperature annealing cavity, a cooling growth cavity and a cooling cavity, wherein the box is separated into the constant-temperature annealing cavity, the cooling growth cavity and the cooling cavity; a gas intake tube, a gas mixing chamber and a reaction cavity are arranged in the constant-temperature annealing cavity and the cooling growth cavity respectively; the gas intake tube stretches in the gas mixing chamber; the gas mixing chamber is communicated with the reaction cavity; a cooling device is arranged in the cooling cavity; a feeding device and a cold gas tube are further arranged in the box; the feeding device penetrates through the reaction cavities and the cooling devices of the constant-temperature annealing cavity and the cooling growth cavity; the constant-temperature annealing cavity, the cooling growth cavity and the cooling cavity are provided with pressure release tubes. The method comprises the following steps: conveying a metal substrate in the constant-temperature annealing cavity and annealing at first; then conveying the metal substrate in the cooling growth cavity and reacting to grow graphene; finally conveying graphene in the cooling cavity and cooling. The equipment and method have the beneficial effects that preparation cycle is short, the equipment is simple in structure and convenient in operation, production efficiency is increased, and high economic benefits are achieved.

The invention discloses a method for preparing a polycrystalline SnSe2 material by a solution method. The method comprises the steps that selenium powder is dispersed in an oleic acid solution to obtain a precursor solution A of a selenium source; then water vapor of the precursor solution A is removed under vacuuming conditions to obtain an environment without oxygen or water vapor; then the temperature rises, heating and heat preservation are conducted, and the precursor solution A is sucked to be added into a mixed solution B composed of a stannum source and ethanol to obtain a mixed solution C, wherein the volume ratio of the precursor solution A to the mixed solution B is (5-7):(25-30); then the mixed solution C is subjected to an alcohol thermal reaction, and drying is conducted to obtain SnSe2 powder. The preparation method is simple, the repeatability is high, the addition of any catalyst is not needed, expensive and complicated equipment is not involved, waste liquid generatedby the reaction is easy to handle, and the method is friendly to the environment and an operator.

The invention discloses a method for preparing SnSe through a solution method. The method comprises the steps that selenium powder is dispersed into a high-boiling-point solvent to obtain a precursorsolution A of a selenium source; then, under the vacuum pumping condition, water vapor in the precursor solution A is removed, and the environment without oxygen and the water vapor is obtained; then,temperature increasing and heating treatment are conducted, the precursor solution A is extracted according to the proportion to be added into a mixed solution B composed of a tin source and ethyl alcohol, thus a mixed solution C is obtained, and then the mixed solution C is subjected to an alcohothermal reaction and then cooled to the room temperature; and through centrifugal separation, cleaning and drying, a black brown target product is obtained. According to the preparation method, the high temperature and high pressure are not needed, no highly toxic and hazardous substance is containedin reaction, inorganic tin salt serves as the tin source, the precursor solution is subjected to the alcohothermal reaction for several hours in a reaction kettle, thus the target product can be obtained, and the product is a purely-flaky nanosheet.

The invention discloses a preparation method and application of a fluorescent probe based on excited-state intramolecular proton transfer and used for accurately measuring monoamine oxidase B in cells. The probe is connected with N-Boc-3-aminopropyl bromide based on a fluorescent dyestuff 2-(2'-hydroxyphenyl)4(3H)quinazolinone of the excited-state intramolecular proton transfer. The probe disclosed by the invention has the advantages that the disadvantages of poor biocompatibility, strong background fluorescent light, easiness for dispersing and the like of a traditional fluorescent probe fordetecting the monoamine oxidase B are overcome, and the probe also has the advantages of good light stability, strong fluorescent light and the like of the 2-(2'-hydroxyphenyl)4(3H)quinazolinone. Theprobe provides an early-stage monitoring means for health and diseases of nervous systems.

The invention provides aluminum-containing high-strength steel and a preparation method and application of the aluminum-containing high-strength steel. The aluminum-containing high-strength steel is prepared from the following chemical components including, by mass: 0.1% to 0.2% of C, 0.3% to 0.6% of Si, 2% to 3% of Mn, less than or equal to 0.0015% of P, less than or equal to 0.0015% of S, 0.5% to 1% of Al, 0.1% to 0.3% of Mo, 0.01% to 0.05% of Pb and the balance of Fe and inevitable impurities. The aluminum-containing high-strength steel provided is good in surface and free of chromatic aberration defects after being galvanized.

The invention discloses a clinical assay reagent for eliminating interference of ascorbic acid in the clinical assay process of oxidizing a substrate by a substrate oxidant. The reagent contains a peroxide, persulfate, sulfite, inorganic ions and acid radical ions with oxidability. The invention also discloses a kit comprising the reagent, a preparation method for the reagent and a method for eliminating the interference of the ascorbic acid in process of detecting a clinical sample through chemical oxidation. The reagent can eliminate the interference of the ascorbic acid in the clinical assay process and improve the accuracy of detection results.

The present application provides a garbage collection method in an all-flash memory array. The garbage collection method includes: deleting HP mapping relationship information in mapping relationshipinformation when it is determined that there is no initial LP mapping to the first space according to mapping relationship information corresponding to the first space in the all-flash memory array; Querying whether there is an LP mapping to the first space according to the mapping relation information; if so, the data in the first space is migrated to the second space, and mapping relationship information corresponding to the second space is established. The existence of LP mapping to the first space is queried, which shows that the host IO writes the same data when deleting the HP mapping relationship information and does not need to wait until the deletion of the HP mapping relationship information is completed. It is possible to avoid having the master IO wait without affecting the response of the service. The present application also provides a garbage collection system in an all-flash memory array, the all-flash memory array and a computer-readable storage medium, all of which have the above beneficial effects.

The invention discloses a clinical examination reagent. The clinical examination reagent can eliminate ascorbic acid interference in chemical oxidation-based clinical sample detection. The clinical examination reagent contains peroxide, persulfate, sulfite, inorganic ions or acid radical ions having oxidability. The invention also discloses a kit containing the clinical examination reagent, a preparation method of the clinical examination reagent, and a method for eliminating ascorbic acid interference in chemical oxidation-based clinical sample detection. The clinical examination reagent can eliminate ascorbic acid interference in clinical detection and improve result accuracy.

The invention discloses a preparation method of a europium-doped strontium aluminate nanobelt with the up-conversion luminescence property. Deionized water is added into aluminum salt and strontium salt to prepare a solution A, then a dopant and ethanol are added into the solution A to prepare a solution B, and then PVP is added into the solution B to prepare a transparent and viscous precursor solution C; then the nanobelt is collected by adopting an electrostatic spinning method; finally the nanobelt is put into a muffle furnace for annealing treatment, and heating, heat preservation and cooling are carried out to obtain a target product SrAl2O4. The electrostatic spinning method is used, and the preparation method has the advantages of simplicity, feasibility, environmental protection and no harm to human bodies; meanwhile, the uniform and fine fiber belt can be obtained directly, and the possibility is provided for more application. The Eu single-doped SrAl2O4 nanobelt is obtained,and green light up-conversion luminescence is realized.

The invention relates to an insulating flame-retardant potting mixture and a preparation method thereof. The potting mixture is composed of an agent A and an agent B in a ratio of (95-105): (35-45) byperforming mixing, wherein the agent A comprises the following components in percentage by mass: 30-40% of bisphenol A epoxy resin, 5-10% of bisphenol F epoxy resin, 15-20% of aluminum hydroxide, 10-15% of silicon dioxide, 5-10% of an environment-friendly flame retardant, 5-10% of benzyl alcohol, 1-2% of an antifoaming agent, 1-2% of a leveling agent, 1-2% of an anti-settling agent and 1-2% of athickener. And the agent B comprises the following components in percentage by mass: 90-95% of an anhydride curing agent and 5-10% of an accelerant. The insulating flame-retardant potting mixture disclosed by the invention is moderate in price and relatively low in production energy consumption, and the preparation method of the insulating flame-retardant potting mixture is simple and low in cost.

The embodiment of the invention belongs to the technical field of medical examination and determination, and relates to an in-vitro diagnostic reagent preservative. The in-vitro diagnostic reagent preservative comprises a composition of a sulfadoxine solution and a diaveridine solution, and the molar ratio of sulfadoxine in the sulfadoxine solution to diaveridine in the diaveridine solution is 0.002 to 1. The invention further relates to application of the in-vitro diagnostic reagent preservative. According to the technical scheme, the stability of the reagent can be improved, reagent reaction is not affected, and the reagent can be widely used.