Application of a two-photon ratiometric fluorescent probe in the detection of monoamine oxidase b

Abstract

The invention discloses a preparation method and application of a two-photon ratio fluorescent probe for accurately measuring monoamine oxidase B in tumor cells. Based on the mechanism of fluorescence resonance energy transfer, the probe uses enzymatic oxidative deamination reaction to generate corresponding iminium ions, which are eliminated after hydrolysis and re-release hydroxyl groups, resulting in proportional changes in fluorescence emission signals. The advantage is that the fluorescent spectrum distance between the energy donor and the acceptor is larger, the interference between the spectra is smaller, and the sensitivity is higher, effectively avoiding the interference of various environmental conditions on the analysis. It provides a potential tool for clinical detection of important marker enzymes of Alzheimer's disease in vivo.

Description

technical field

[0001] The present invention relates to small molecule fluorescent probes to identify the location and expression level of abnormal biological enzyme activity in cancer cells, more specifically, a two-photon naphthalene derivative fluorescent probe based on fluorescence resonance energy transfer for Alzheimer's disease Detection of monoamine oxidase B (MAO-B), an important marker of the disease. Background technique

[0002] Alzheimer's disease (AD) is the most common dementia among older adults, currently affecting more than 35 million people worldwide. In particular, the clinical detection of related biomarkers is of great significance for the early diagnosis, treatment and management of diseases. Monoamine oxidase B (MAO-B) is a mitochondria-associated flavin enzyme highly expressed in the human brain, mainly in astrocytes, where it helps maintain the homeostasis of neurotransmitters in the brain, thereby ensuring proper neural and behavioral outcomes. ...

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