A method, reagent and kit for eliminating ascorbic acid interference in clinical testing
A technology for clinical testing and ascorbic acid, applied in the field of clinical testing reagents, can solve the problems of ascorbic acid interference, poor anti-interference ability of ascorbic acid, abnormal measurement results, etc., and achieve the effect of increasing reliability
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Embodiment 1
[0050] Embodiment 1 The elimination ascorbic acid interference test of peroxide
[0051] Formula Table
[0052] Reagent 1 (pH3.0)
[0053]
[0054] The specific dosage of carbamide peroxide (or hydrogen peroxide, or peracetic acid) is shown in Table 1
[0055] h 2 O to 1.0L
[0056] Reagent 2 (pH7.0)
[0057] Sodium dihydrogen phosphate 1.0g
[0058] Sodium metavanadate 0.5g
[0059] h 2 O to 1.0L
[0060] The method for determining direct bilirubin in a sample by using the embodiment of the present invention is as follows: add reagent 1 to the sample (calibration tube as a sample) and mix well, and read the absorbance A1 after incubating at 37°C for 3 to 5 minutes; add reagent 2, mix well , read the absorbance A2 after reacting at 37°C for 5 minutes; according to the formula: direct bilirubin content = measured absorbance (A2-A1) × calibration solution / calibration absorbance (A2-A1) to obtain the direct bilirubin content. Wherein, the measurement wavelength is 4...
Embodiment 2
[0066] Embodiment 2 Elimination of ascorbic acid interference test of persulfate
[0067] Formula Table
[0068] Reagent 1 (pH3.0)
[0069]
[0070]
[0071] The specific dosage of potassium persulfate (or sodium persulfate) is shown in Table 2
[0072] h 2 O to 1.0L
[0073] Reagent 2 (pH7.0)
[0074] Sodium dihydrogen phosphate 1.0g
[0075] Sodium metavanadate 0.5g
[0076] h 2 O to 1.0L
[0077] Take by weighing the material in the formula table, take by weighing persulfate wherein by the amount in table 2, by the preparation method among the embodiment 1, prepare the test kit (prescription 12-17) that contains different kinds and concentration persulfate respectively , Formulation 11 is a persulfate-free kit. The sample and detection method for detection are the same as in Example 1. The results are shown in Table 2. The results show that when using the kit containing the persulfate to detect ascorbic acid samples, the interference deviation between the t...
Embodiment 3
[0080] Embodiment 3 Elimination of ascorbic acid interference test of sulfite
[0081] Formula Table
[0082] Reagent 1 (pH3.0)
[0083]
[0084] The specific dosage of sodium sulfite (or sodium bisulfite) is shown in Table 3
[0085] h 2 O to 1.0L
[0086] Reagent 2 (pH7.0)
[0087] Sodium dihydrogen phosphate 1.0g
[0088] Sodium metavanadate 0.5g
[0089] h 2 O to 1.0L
[0090] Take by weighing the material in the formula table, take by weighing the sulfite wherein in the amount in table 3, by the preparation method in the embodiment 1, prepare the test kit (prescription 19-24) that contains different kinds and concentrations of sulfite respectively , Formula 18 is a sulfite-free kit. The sample and detection method for detection are the same as in Example 1. The results are shown in Table 3. The results show that when using the kit containing the sulfite to detect the ascorbic acid sample, the interference deviation between the test result and the blank sampl...
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