Method for detecting residual quantity of antivirus drug in hairs of livestock and poultry
A residue and hair technology, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as low detection limits
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Embodiment 1
[0103] Wash the pig hair with tap water to remove visible impurities, ultrasonicate the hair in 1% SDS+5% Tween 80+pure water (1:1:1) for 15 minutes, rinse it with distilled water, and dry it with filter paper; dry the hair at 50°C, put Store in a desiccator.
[0104] Cut into 1-2mm pieces with scissors, weigh 0.5g of hair into a 50mL centrifuge tube, add 50.0μL of D15-amantadine standard working solution, 5mL of 0.2mol / L HCI solution, mix well, digest at 65°C for 2 hours, take out , add 8mL 2.0% trichloroacetic acid aqueous solution + acetonitrile (1:2), vortex for 30s, add 2g sodium chloride after ultrasonic extraction for 40min, vortex mix for 2min, centrifuge at 5000r / min for 10min, take the supernatant in another In the tube, dry it with nitrogen at 60°C, add 3mL of 2% formic acid aqueous solution to dissolve, and load the solution into the MCX column that has been activated with 3mL of methanol and 3mL of water, and then rinse with 3mL of 2% formic acid aqueous solution ...
Embodiment 2
[0109] Wash the chicken feathers with tap water to remove visible impurities, sonicate the hair in 1% SDS+5% Tween 80+pure water (1:1:1) for 15 minutes, rinse it with distilled water, and dry it with filter paper; dry the hair at 50°C and let it dry spare in the device.
[0110] Cut into 1-2mm pieces with scissors, weigh 0.5g of hair into a 50mL centrifuge tube, add 50.0μL of D15-amantadine standard working solution, 5mL of 0.2mol / L HCI solution, mix well, digest at 65°C for 2 hours, take out , add 8mL 2.0% trichloroacetic acid aqueous solution + acetonitrile (1:2), vortex for 30s, add 2g sodium chloride after ultrasonic extraction for 40min, vortex mix for 2min, centrifuge at 5000r / min for 10min, take the supernatant in another In the tube, dry it with nitrogen at 60°C, add 3mL of 2% formic acid aqueous solution to dissolve, and load the solution into the MCX column that has been activated with 3mL of methanol and 3mL of water, and then rinse with 3mL of 2% formic acid aqueou...
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