Recombinant canine distemper virus (rCDV) containing canine GMCSF (granulocyte macrophage colony stimulating factor) gene and preparation method thereof

A canine distemper virus and genome technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, viruses/phages, etc., can solve problems such as the inability to completely control canine distemper, and achieve the effect of improving immunogenicity
CN104164409AInactive Publication Date: 2014-11-26MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Publication Date
2014-11-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of recombinant virus vaccines, and discloses recombinant canine distemper virus (rCDV) containing canine GMCSF (granulocyte macrophage colony stimulating factor) gene, and more specifically, the recombinant canine distemper virus is rCDV-GMCSF. The invention also discloses a preparation method of the recombinant canine distemper virus. According to the present invention, reverse genetics operation technology is used for inserting the canine GMCSF gene into a canine distemper virus genome to construct full-lengthen complementary DNA (cDNA) clone pCI-CDV-GMCSF of the recombinant genome containing the canine GMCSF gene; RT-PCR (reverse transcription-polymerase chain reaction), indirect immunofluorescence assay and electron microscopy observation confirm that the recombinant canine distemper virus rCDV-GMCSF for expression of GMCSF protein is successfully rescued, the growth titer of the recombinant canine distemper virus has no significant difference with that of wild type canine distemper virus, and a material basis for further study on application of the GMCSF as an adjuvant in canine distemper attenuated vaccine strain is laid.
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Description

technical field

[0001] The present invention relates to the field of recombinant virus vaccines, more specifically, the present invention relates to a recombinant canine distemper virus containing a gene encoding canine granulocyte-macrophage colony-stimulating factor, more specifically, the recombinant canine distemper virus is rCDV- GMCSF. The invention also discloses a preparation method of the recombinant canine distemper virus. Background technique

[0002] Canine distemper is an acute, febrile, highly contact and fatal infectious disease caused by Canine Distemper Virus (CDV) in various animals. It often causes diseases in dogs, minks, foxes and other animals. The animal breeding industry has caused huge losses, and the hosts of natural infection of CDV are constantly expanding. In addition to canines, it can also infect all 8 families of carnivores such as giant pandas, red pandas, lions, tigers and leopards, artiodactyla Suidae, and primates Various animals such as...

Claims

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