Improved method for determining content of amino acids

A technology of amino acid content, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of easily generating foam and affecting the judgment end point, etc., and achieve the effect of easy operation and simple steps

Inactive Publication Date: 2014-11-26
广州衡创测试技术服务有限公司
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Problems solved by technology

In the traditional method, hydrochloric acid is added to dissolve the sample during pretreatment, which is prone to foaming and will affect the determination of the end point when the volume is constant.

Method used

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Embodiment Construction

[0027] In order to allow those skilled in the art to understand the present invention more clearly and intuitively, the present invention will be further described below.

[0028] An improved method for determining amino acid content, comprising the following steps:

[0029] (1) Accurately weigh 0.1000g of the sample into a 10ml ampoule, then accurately add 5ml of 1+1 hydrochloric acid to the ampoule, seal the ampoule, and wrap the ampoule with adhesive tape after cooling to prevent digestion at a constant temperature. The ampoule was broken and digested in a constant temperature drying oven at 110°C for 12 hours;

[0030] (2) Take out the ampoule and cool to room temperature, cut open the ampoule, filter the digestive juice, and immediately pipette 1mL of the filtrate accurately into a 50mL volumetric flask. At this time, there are many foams in the volumetric flask, add 2-3 drops of ethanol to defoam, and then add water Dilute to the mark;

[0031] (3) The diluted solution...

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Abstract

The invention provides an improved method for determining the content of amino acids. The method comprises the following steps: processing a test sample, and mixing and digesting the test sample and hydrochloric acid in an ampoule bottle; after digesting and cooling, adding ethyl alcohol for defoaming, and adding water for diluting and then filtering for later use; diluting a standard sample for 25 times with sodium citrate for later use; preparing a mobile phase A, a mobile phase B and a mobile phase C, and then preparing reaction liquid A and reaction liquid B; respectively testing and analyzing the test sample and the standard sample on a high performance liquid chromatograph; according to peak appearance time, identifying the types of amino acids in the sample; and comparing the peak area of the sample with that of the standard sample, and calculating the content of various amino acids in the sample. According to the method, ethyl alcohol is added for defoaming, and the judgment of constant volume calibration and the result cannot be influenced. The method is simple in step and easy to operate.

Description

technical field [0001] The invention relates to a method for determining amino acid content, in particular to an improved method for determining amino acid content. Background technique [0002] Amino acids have the function of preventing and curing diseases in medicine, and can also be used as active ingredients in nutritional cosmetics to synthesize drugs, surfactants and other raw materials for industrial products. Therefore, amino acid analysis is one of the most important techniques in industry, agricultural production and life science research. [0003] The protein in feed was hydrolyzed with hydrochloric acid to make it into free amino acid, and its composition was analyzed by post-column derivatization of high performance liquid chromatography. In the traditional method, hydrochloric acid is added to dissolve the sample during pretreatment, which is prone to foaming and will affect the determination of the end point when the volume is constant. Contents of the inv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张玉良陈凯松
Owner 广州衡创测试技术服务有限公司
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