48results about How to "Will not affect the result" patented technology

The invention relates to a method for removing oxytetracycline in biological medicine waste residues. The steps of pretreatment, catalytic oxydative degradation, bacterium inactivation, neutralizing treatment and the like are adopted, and an organic matter waste residue product containing no oxytetracycline can be formed after drying and can be used for preparing subsequent soil conditioners and organic and inorganic compound fertilizer. Compared with the prior art, purchase, use and management are easy, and hydrogen peroxide is convenient to use; and the method is used for treating the biological medicine waste residues, the oxytetracycline waste residue cost is low, the treatment process is fast, environment-friendly and small in harm, finally, the biological medicine waste residues are used as raw materials of the organic fertilizer or the soil conditioners, and the utilization value is high.

The invention relates to a long-acting ELISA (Enzyme-linked Immunosorbent Assay) plate stabilizing agent for keeping protein activity in an ELISA plate. The agent comprises 0.1-5g protein, 1-15g sugar, 0.1-10g organic matter, 0.01-0.1%(V/V) of preservative, and 10-200mmol buffer solution salt per 100ml water, and a pH (Potential of Hydrogen) value is 7.2-7.4. The stabilizing agent adopts a technical route integrating a closing process and a stabilization process, and can effectively close excessive sites in the ELISA plate, and some active ingredients in the stabilizing agent can ensure continuous stabilization of the ELISA plate. The agent is simple in formula, can stabilize the ELISA plate, and does not produce any influence on a result simultaneously. The ingredients of the agent are easy to obtain, and the formula is simple, so that the cost of the stabilizing agent is lowered.

The invention discloses a method for detecting pathogens of infectious diseases, which possibly exist in a biological sample. The pathogens of the infectious diseases comprise avian influenza virus H5 subtype, avian influenza virus H7 subtype, SARS coronavirus, hanta virus, plague yersinia pestis and bacillus anthracis. The method comprises amplifying a nucleic acid fragment of a biological sample and detection by a probe. The invention also provides a primer for amplification and a probe for detection. The invention also provides a kit including the primer. The method has the advantages of high flexibility, strong specificity, easy operation, wide sample range, the detection for the pathogens of various infectious diseases at the same time and the suitability for early diagnosis of respiratory infectious diseases.

The invention discloses a method for detecting infectious disease pathogens possibly existing in a biological sample. The infectious disease pathogens comprise chlamydia pneumoniae, haemophilus influenzae, mycoplasma pneumoniae, pneumoniae streptococcus and legionella pneumophila. The method comprises the following steps: expanding nucleic acid fragments of the biological sample, and detecting the nucleic acid fragments by using a probe. The invention also provides primers used for expanding and the probe used for detection. The invention also provides a kit comprising the primers. The method has the advantages of high sensitivity, strong specificity, simple operation and wide sample range, can simultaneously detect various infectious disease pathogens, and is suitable for early diagnosis of respiratory infectious diseases.

The invention relates to a use of a piperazinopyrimidine isotope labeling reagent. The piperazinopyrimidine isotope labeling reagent is a light/heavy isotope labeling reagent of [d0]-/[d6]-2,4-dimethoxy-6-piperazinopyrimidine. The piperazinopyrimidine isotope labeling reagent can be used for comparison analysis of carboxyl compounds such as fatty acid, polypeptides or proteins. Fatty acid analysis comprises the following steps of a) fatty acid reacts respectively with light and heavy labeling reagents; after after-treatment, an LC-MS/MS analysis process is carried out; and product ions m/z 225 and m/z 231 are selected as quantitative ions; and b) through observation of pairs of mass spectrum peaks of the labeled fatty acid, identification of a plurality of carboxyl fatty acids is realized and quantification is realized according to relative peak areas. Polypeptide/protein comparison analysis comprises the following steps of a) a protein needing to be analyzed is subjected to thermal denaturation and enzymolysis and then reacts with the light/heavy labeling reagent; and b) the reaction solution is subjected to after-treatment; and a MALDI-TOF MS comparison analysis process is carried out.

The invention discloses a method for detecting infectious disease pathogens possibly existing in a biological sample. The infectious disease pathogens comprise EB virus, herpes simplex virus, cytomegalic inclusion disease virus and adenovirus. The method comprises the following steps: expanding nucleic acid fragments of the biological sample, and detecting the nucleic acid fragments by using a probe. The invention also provides primers used for expanding and the probe used for detection. The invention also provides a kit comprising the primers. The method has the advantages of high sensitivity, strong specificity, simple operation and wide sample range, can simultaneously detect various infectious disease pathogens, and is suitable for early diagnosis of respiratory infectious diseases.

The invention discloses an upwards-dialing and cutting type coal sample division device, comprising a division device body which includes an outer frame, a conical reshaper, a division dialing plate and a split baffle, wherein the top of the outer frame is provided with a sample inlet, the shape of the top of the conical reshaper is matched with that of the sample inlet, the area of the top of the conical reshaper is more than that of the bottom of the same, the lower end of division dialing plate is fixed on the outer frame by a rotary mechanism and above the split baffle , and the bottom of the outer frame is divided into two sample outlets by the split baffle. The method also provides two division methods; through the two division methods, divided samples can meet international requirements, in the division process, no leakage points exist; as the division dialing plate upwards dials and cuts coal samples, the coal flow has short contact time and area in the division device, the divided coal samples are representative, and the problems of moisture loss, particle size segregation and being easy to cause dust are overcome; the upwards-dialing and cutting type coal sample division device has greatly improved division precision than existing equipment.

An embodiment of the invention discloses a media data processing method. The method comprises the steps of obtaining to-be-uploaded media when a terminal device receives an instruction of uploading the media to a server; storing the to-be-uploaded media in a local cache of the terminal device by the terminal device, and generating a first call address capable of calling the media from the cache; adding the first call address to an HTML tag corresponding to the media by the terminal device; and performing rendering on the media according to the HTML tag by the terminal device, and presenting arendering result on a display interface of the terminal device. An embodiment of the invention discloses the terminal device. The terminal device can finish the rendering of to-be-processed media information offline without uploading the to-be-processed media information to the server in real time and requesting the server to send the first call address, and the display speed and result are not influenced even under the condition of relatively poor network quality.

The invention discloses a loop-mediated isothermal amplification detection primer set, method and kit for detection of Xanthomonas oryzae. Based on genome specific molecular marker characteristics ofXanthomonas oryzae, conservative specific DNA fragment xcp of Xanthomonas oryzae is acquired by means of a comparative genomics method; diseased rice seedlings and seeds are successfully detected through loop-mediated isothermal amplification; the method herein is quick, simple and efficient. The whole detection process has no need for special instruments and can be finished within 45-90 min in afield or lab. The method is high in sensitivity, suitable for detecting rice materials containing down to 10 bacteria, good in specificity, and is free of influence upon results due to other pathogensor plant genomes. The method is good in safety and free of harm and employs no organic agents or nucleic acid binding dyes; all agents are directly releasable to the environment; the method is suitable for detecting all possible pathogen propagating sources.

The invention provides an improved method for determining the content of amino acids. The method comprises the following steps: processing a test sample, and mixing and digesting the test sample and hydrochloric acid in an ampoule bottle; after digesting and cooling, adding ethyl alcohol for defoaming, and adding water for diluting and then filtering for later use; diluting a standard sample for 25 times with sodium citrate for later use; preparing a mobile phase A, a mobile phase B and a mobile phase C, and then preparing reaction liquid A and reaction liquid B; respectively testing and analyzing the test sample and the standard sample on a high performance liquid chromatograph; according to peak appearance time, identifying the types of amino acids in the sample; and comparing the peak area of the sample with that of the standard sample, and calculating the content of various amino acids in the sample. According to the method, ethyl alcohol is added for defoaming, and the judgment of constant volume calibration and the result cannot be influenced. The method is simple in step and easy to operate.

The invention discloses an accurate positioning test paper for urine testing. The test paper includes a test piece and a white long-strip thin substrate, wherein the white long-strip thin substrate includes an integrally-formed rectangular plate portion and a handle portion, the test piece is disposed on the rectangular plate portion, the test piece is provided with multiple equally-spaced small test papers, the rectangular plate portion is located outside each small test paper and close to the small test paper and is provided with a black outer frame, a middle portion of the test piece is provided with an insertion prompt line in the width direction, a middle and lower edge of the test piece is provided with an arrow prompt icon in the length direction, a mark of this face up is arrangedbelow the arrow prompt icon, and a round transition is arranged at a corner of the test piece. The test paper is positioned accurately, and the detection result has high precision.

The invention discloses a watermelon fertilizing method, and belongs to the technical field of fruit fertilizing. The method comprises the following steps: 1, base fertilizer application: applying farmyard manure to soil, adding plant ash and calcium superphosphate, uniformly mixing with soil, covering soil, and planting or directly sowing ; and 2, additional fertilization: carrying out first time fertilizer application after directly sowed sprouts of watermelon seedlings have two true leaves or transplanted sprouts recover, applying urea to the sprouts as a seedling assisting fertilizer when the watermelon sprouts vine, applying a ternary composite fertilizer between the sprouts after the sprouts vine, carrying out trenching urea and potassium sulfate fertilizer application during the appearance of young fruits of the watermelons, and respectively spraying 0.5% potassium dihydrogen phosphate and 0.5% calcium nitrate two times before maturation. Fertilization is carried out according to the difference of nutrients required by the watermelons in different stages, and sufficient nutrients are provided for the watermelon sprouts in each stage in order to make the watermelon sprouts rapidly grow, so high yield and economic benefit improvement are realized.

The invention relates to a purity evaluation device for electronic-grade trichlorosilane. The device comprises a chassis, a bell-jar, which is arranged on the chassis and has a cooling water jacket, a tantalum filament, which is arranged in the bell-jar with the cooling water jacket, and a pair of electrodes, which go through the chassis and are connected to the power supply. The top part of the bell-jar with the cooling water jacket is provided with a hanger and a bell-jar cooling water outlet, the side wall of the bell-jar with the cooling water jacket is provided with a bell-jar cooling water inlet and a sight glass; the two electrodes are connected through the tantalum filament, the outside of the pair of electrodes is provided with a reaction gas inlet and a reaction gas outlet, and the reaction gas inlet and the reaction gas outlet go through the chassis and communicate with the cavity of the bell-jar with the cooling water jacket. The purity evaluation device for electronic-grade trichlorosilane has the advantages of simple structure and low cost, achieves the goals of rapid and precise purity measurement, and realizes safety operation.

The invention discloses a program-controlled variable five-bit nonreciprocal microwave monolithic integrated attenuator, which consists of an input port standing wave regulation module, a microwave attenuation module, an output port standing wave regulation module and a control module (21), wherein the input port standing wave regulation module is completely symmetrical with the output port standing wave regulation module. The program-controlled variable five-bit nonreciprocal microwave monolithic integrated attenuator has the advantages that: all attenuation networks are connected in parallel, the loss of microwave signals is low; all control switches and all microwave single-pole single-throw switches are realized by adopting small gallium arsenide field effect transistors, and have high switching performance, and the operation bandwidth of the attenuator is wide; an attenuation circuit is connected in parallel with a microwave amplitude balancing circuit, so the amount of attenuation in the whole working band is balanced and flat; attenuation control coding is irregular, and if a certain bit is shifted, results may not be influenced greatly; and a novel structure is introduced to reduce the sensitivity of a process and then the yield of a chip is increased from 60 percent to 80 percent.

The invention discloses a program-controlled variable four-digit microwave one-chip integrated attenuator. The attenuator is composed of an input port standing wave adjusting module, a microwave attenuation module, an output port standing wave adjusting module and a control module (21); impedances of all the ports are normalized to 50 ohms; and the ports can be utilized in cascade connection to realize high attenuation. The attenuator provided in the invention has the following advantages that: all attenuation networks are in parallel connection, so that there are few losses on a microwave signal; all control switches and all microwave single-pole single-throw switches employ GaAs field effect transistors, which are extremely small, so that the switch performances are good and a working bandwidth of the attenuator is very wide; an attenuation circuit and a microwave amplitude balancing circuit are in parallel connection, so that the attenuation in a whole working frequency band is substantially balanced and flat; there is no rule for attenuation control coding, so that there is no great influence on results caused by deviation of a certain digit; And introduction of a novel structure enables sensitiveness of the technology to be reduced and a chip yield to be improved from 60% to 80%.

The invention provides an experimental measurement device and method for an additional mass matrix between circular and cylindrical members of an annular fluid region of a reactor, simplification and scaling are carried out according to a fast reactor model, and five branch circular bearing cylinders are symmetrically placed in a semi-annular region device with an included angle of 180 degrees relative to a main pump bearing cylinder according to a placement relation in a fast reactor body; the semi-annular region device is filled with water, each bearing cylinder is provided with three layers of measuring points, the measuring points are all underwater, and each layer of measuring points is provided with an acceleration sensor and a pressure sensor for measuring acceleration and pressure. A vibration exciter is used for carrying out vibration excitation on each bearing cylinder, and an additional mass matrix is obtained through calculation according to a formula of fluid acting force borne by a plurality of interacting entities in water. According to the invention, the obtained result is more accurate and stable by selecting the sensors and the measuring point positions in a targeted manner. By adopting the measurement mode of the device, the fluid-solid coupling characteristic between adjacent cylinders in a limited drainage basin can be better evaluated.

The invention discloses application of A mass spectrum technology to detection of molecular level of PSM-E in urine in preparation of product for early diagnosis of prostate cancer A urine protein sample is diluted and then is directly introduced into a liquid chromatography-electrospray ionization-tandem mass spectrometer for determination, and the content level of the PSM-E in the urine can be rapidly and accurately detected; a fully-synthesized PSM-E characteristic peptide standard substance is adopted as an internal standard quantitative analysis substance, so that errors caused in a sample pretreatment process are reduced; the selectivity and the accuracy of the method are better improved by the liquid chromatography-tandem mass spectrometry; through selection and optimization of a chromatographic column and elution conditions, the chromatographic separation process is improved, and the chromatographic analysis time is shortened. According to the invention, the quantitative detection of the PSM-E in the urine sample can be realized, the operation is simple, the application is convenient, the content of the PSM-E in the urine can be used as a specific biomarker for detecting the prostatic cancer, and an efficient and feasible technical method is provided for early screening of the prostatic cancer.