The invention belongs to the field of fine chemical extraction, and in particular relates to an extraction treatment method for detection and analysis of vitamin A in food. First, the food sample is freeze-dried to remove water, then the freeze-dried food sample is added to the chloroform-ethanol mixture and homogenized at high speed, then the homogenized sample is centrifuged and the chloroform extract located in the lower layer is collected, and the extract is dried with nitrogen. Ethanol is added to reconstitute and purify the vitamin A, and ultrasonic waves are used in the redissolution step to completely dissolve and extract the vitamin A. The extraction of the present invention does not require processes such as lye saponification and degreasing and vacuum distillation in the traditional method, and also overcomes the problems of incomplete dehydration of food samples caused by adding anhydrous sodium sulfate to grind and absorb water, and has the advantages of simple operation and shortened time , high extraction efficiency, etc., and also overcomes the danger of using ether, n-hexane or methanol as the extraction solvent. Due to the water solubility of ethanol, even trace amounts of water in the extracted sample will not affect the results.