A method for extracting and processing vitamin A detection and analysis in food

A treatment method, vitamin technology, applied in the preparation of test samples, etc., can solve the problems of human body damage, easy volatilization, and high toxicity of extraction solvents, and achieve the effects of overcoming danger, high extraction efficiency, and easy operation

Inactive Publication Date: 2016-03-09
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method omits the saponification process, there is incomplete water removal in the sample, which may affect the extraction efficiency of vitamin A by organic solvents such as ether.
Moreover, in the currently applied methods, ether, n-hexane or methanol are used as the extraction solvent, and there is a risk that the extraction solvent is highly toxic, volatile, and likely to cause damage to the human body.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Cleaning, cutting and crushing: Take 100 mg of pork liver washed and dried with tap water, put it in a 15 mL test tube, and freeze-dry it in a freeze dryer for 2 hours, so that the residual moisture content in the sample is less than 2%.

[0025] 2. Chloroform ethanol extraction:

[0026] 1) Add 3 mL of chloroform-ethanol mixture (chloroform:ethanol=2:1) ​​to the freeze-dried sample.

[0027] 2) Use a high-speed homogenizer at 8000 rpm, homogenize for 2 minutes, completely break up the pig liver and mix it well with the solvent. After extracting in the dark for 2 hours, add 1 mL of normal saline, and shake with a vortex mixer at 2800 rpm for 30 seconds.

[0028] 3) After oscillating and mixing, centrifuge in a refrigerated centrifuge at 4°C and 3000 rpm for 5 minutes, take the chloroform extract from the lower layer and add it to another clean 10mL test tube.

[0029] 4) Add 3 mL of chloroform-ethanol mixture to the remaining sample residue to repeat the extraction...

Embodiment 2

[0039] 1. Sample freeze-drying: Take 1mL milk sample and place it in a 15mL test tube, freeze-dry for 2 hours, so that the residual moisture content in the sample is less than 2%.

[0040] 2. Chloroform ethanol extraction:

[0041] 1) Add 3 mL of chloroform-ethanol mixture (chloroform:ethanol=2:1) ​​to the freeze-dried sample.

[0042] 2) After leaching in the dark for 2 hours, add 1 mL of normal saline, and shake with a vortex mixer at 2800 rpm for 30 seconds.

[0043] Refrigerated centrifuge at 4°C, 3000 rpm, and centrifuge for 5 minutes.

[0044] 3) Remove the chloroform extract from the lower layer and add it to another clean 10mL test tube.

[0045] 4) Add 3 mL of chloroform-ethanol mixture to the remaining sample residue to repeat the extraction once, and combine the two extracts.

[0046] 3. Ethanol reconstitution and purification:

[0047] 1) Place the test tube containing the above combined extracts in a water bath at 26°C, and blow dry with nitrogen.

[0048] 2)...

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PUM

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Abstract

The invention belongs to the field of fine chemical extraction, and in particular relates to an extraction treatment method for detection and analysis of vitamin A in food. First, the food sample is freeze-dried to remove water, then the freeze-dried food sample is added to the chloroform-ethanol mixture and homogenized at high speed, then the homogenized sample is centrifuged and the chloroform extract located in the lower layer is collected, and the extract is dried with nitrogen. Ethanol is added to reconstitute and purify the vitamin A, and ultrasonic waves are used in the redissolution step to completely dissolve and extract the vitamin A. The extraction of the present invention does not require processes such as lye saponification and degreasing and vacuum distillation in the traditional method, and also overcomes the problems of incomplete dehydration of food samples caused by adding anhydrous sodium sulfate to grind and absorb water, and has the advantages of simple operation and shortened time , high extraction efficiency, etc., and also overcomes the danger of using ether, n-hexane or methanol as the extraction solvent. Due to the water solubility of ethanol, even trace amounts of water in the extracted sample will not affect the results.

Description

technical field [0001] The invention belongs to the field of fine chemical extraction, and in particular relates to an extraction treatment method for detection and analysis of vitamin A in food. Background technique [0002] Vitamin A is rich in animal liver, fish eggs, whole milk, butter, poultry eggs and other foods, with the highest content in beef liver and sheep liver, and is usually dissolved in the lipids of food. Plants do not contain formed vitamin A, but contain carotenoids, a small part of which can be converted into vitamin A in humans and animals, so they are also called pre-vitamin A or provitamin A. [0003] Due to the fat solubility of vitamin A, choosing an appropriate solvent and method to extract and separate it from food lipids is a key link in the determination and analysis of vitamin A. The moisture in food will also affect the full contact between the extraction solvent and lipids , thus hindering the extraction and separation of vitamin A from lipid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34
Inventor 李蕾苏妍文李宇晴陈小旋江玲玲钟进义
Owner XIAMEN UNIV
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