Use of piperazinopyrimidine isotope labeling reagent

A technology of isotope labeling and pyrimidine, which is applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of difficult qualitative and quantitative detection, weak content, weak mass spectrometry response, etc., to improve the product mass spectrometry response intensity, The background noise of the product is low and the effect of avoiding signal interference
CN102944604BInactive Publication Date: 2015-02-18SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
Publication Date
2015-02-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a use of a piperazinopyrimidine isotope labeling reagent. The piperazinopyrimidine isotope labeling reagent is a light / heavy isotope labeling reagent of [d0]- / [d6]-2,4-dimethoxy-6-piperazinopyrimidine. The piperazinopyrimidine isotope labeling reagent can be used for comparison analysis of carboxyl compounds such as fatty acid, polypeptides or proteins. Fatty acid analysis comprises the following steps of a) fatty acid reacts respectively with light and heavy labeling reagents; after after-treatment, an LC-MS / MS analysis process is carried out; and product ions m / z 225 and m / z 231 are selected as quantitative ions; and b) through observation of pairs of mass spectrum peaks of the labeled fatty acid, identification of a plurality of carboxyl fatty acids is realized and quantification is realized according to relative peak areas. Polypeptide / protein comparison analysis comprises the following steps of a) a protein needing to be analyzed is subjected to thermal denaturation and enzymolysis and then reacts with the light / heavy labeling reagent; and b) the reaction solution is subjected to after-treatment; and a MALDI-TOF MS comparison analysis process is carried out.
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Description

technical field

[0001] The invention relates to a design and synthesis method of a piperazinyl pyrimidine isotope labeling reagent and its application in mass spectrometry analysis of carboxyl-containing compounds. In particular, the combination of liquid chromatography mass spectrometry and matrix-assisted laser desorption mass spectrometry can achieve fast, efficient and accurate mass spectrometry analysis, which provides a good means for high-throughput research on carboxyl compounds, and is even more important for mass spectrometry of biomarkers in diagnostic medicine. Analysis provides a strategy. technical background

[0002] One of the important topics in diagnostic medical research is to discover and monitor new parameters of human diseases—biomarkers (see J.Clin.Pharmacol.2003,43,329.). For disease research, biomarkers generally refer to certain characteristic biochemical indicators in a common physiological or pathological or therapeutic process that can be object...

Claims

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