Extraction method of hyoscyamine
An extraction method, the technology of scopolamine, applied in the field of phytochemistry, can solve the problems of large energy consumption, large environmental pollution, and high production cost of chemical synthesis
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Embodiment 1
[0024] The fresh raw material was dried in an oven at 60° C. for 1 hour, crushed through a 40-mesh sieve, and the coarse powder was collected for later use. Weigh 20g of material coarse powder, add 0.3g of cellulase, and add 40ml of water to infiltrate, and enzymolyze it for 1h under the conditions of pH 4 and temperature 45°C to obtain a coarse powder enzymatic hydrolyzate. The hydrolyzate was placed in a Soxhlet extractor, 80ml of ethanol was added, and alcohol extraction was carried out using the principle of solvent reflux and siphon. The crude extract of scopolamine was obtained. The obtained hyoscyamine crude extract is eluted on a D101 type anion exchange resin column, first eluted with distilled water of 3 times the column volume, and then eluted with 4 times the column volume of ethanol, and the ethanol eluate is collected and concentrated under reduced pressure to 1 / 5 of the original volume, namely hyoscyamine. After testing, the content of scopolamine in the obtai...
Embodiment 2
[0026] The fresh raw materials were dried in an oven at 65°C for 2 hours, crushed through a 60-mesh sieve, and the coarse powder was collected for later use. Weigh 20g of material coarse powder, add 0.5g of hemicellulase, and add 80ml of water to infiltrate, enzymatically hydrolyze for 3 hours under the conditions of pH 5 and temperature 47°C to obtain the coarse powder enzymatic hydrolyzate, and the obtained coarse powder Put the enzymatic hydrolyzate in a Soxhlet extractor, add 100ml of ethanol, use the principle of solvent reflux and siphon to carry out alcohol extraction, first reflux the sample for 2 times, and finally soak it in the solvent for 22h, and then continue to extract for 2h , to obtain the crude extract of scopolamine. The obtained hyoscyamine crude extract is eluted on a D101 type anion exchange resin column, first eluted with distilled water of 4 times the column volume, and then eluted with 4 times the column volume of ethanol, and the ethanol eluate is col...
Embodiment 3
[0028] The fresh raw materials were dried in an oven at a temperature of 70° C. for 3 hours, crushed through a 60-mesh sieve, and the coarse powder was collected for later use. Weigh 20g of raw material powder, add 0.7g of pectinase, and add 100ml of water to infiltrate, and enzymolyze it for 4 hours at a pH of 5 and a temperature of 50°C to obtain an enzymatic hydrolyzate of the coarse powder. The hydrolyzate was placed in a Soxhlet extractor, 200ml of ethanol was added, and it was extracted by solvent reflux and siphon. The crude extract of scopolamine was obtained. The obtained hyoscyamine crude extract is eluted on a D101 type anion exchange resin column, first eluted with distilled water of 5 times the column volume, and then eluted with 10 times the column volume of ethanol, collecting the ethanol eluate, and concentrating under reduced pressure to 1 / 2 of the original volume, namely hyoscyamine. After testing, the content of scopolamine in the obtained sample was 71.62...
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