Mouthwash containing enzyme and pericarpium citri reticulatae extract and preparation method thereof

A technology of tangerine peel extract and mouthwash, applied in the field of oral care, can solve the problems of less attention to the effect of dental plaque removal, etc., and achieve the effect of remarkable effect, easy realization, and simple production process

Active Publication Date: 2014-12-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current oral rinses focus on the elimination of individual oral bacteria, such as adding lysozyme to steril

Method used

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  • Mouthwash containing enzyme and pericarpium citri reticulatae extract and preparation method thereof
  • Mouthwash containing enzyme and pericarpium citri reticulatae extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) The preparation method of tangerine peel extract:

[0039] In 30mL pre-cooled ethanol solution (mass percentage concentration is 80%), add 2.0g dry tangerine peel powder, obtain dispersion liquid; With the rotating speed of 12000rpm, dispersion liquid homogeneous 5min is in high-speed homogenizer; Adopt centrifuge to Centrifuge at 3000rpm for 3min and collect the supernatant;

[0040] Add 30mL pre-cooled ethanol solution (mass percentage concentration is 80%) to continue homogenization (rotating speed is 12000rpm, homogenization time is 5min), centrifugation (centrifugal speed is 3000rpm, centrifugal time is 3min) to the precipitate after centrifugation, take Supernatant, so repeated 5 times, combined supernatant.

[0041] Concentrate the combined supernatant in a rotary evaporator until there is no flowing liquid in the concentrate to obtain the orange peel concentrate, which is dissolved in 10 mL of reverse osmosis purified water, and stored at -40°C for later us...

Embodiment 2

[0048] (1) The preparation method of tangerine peel extract:

[0049] In 30mL pre-cooled ethanol solution (mass percentage concentration is 90%), add 2.0g dry tangerine peel powder, obtain dispersion liquid; With the rotating speed of 11000rpm, dispersion liquid homogeneous 8min is in high-speed homogenizer; Adopt centrifuge to Centrifuge at 4000rpm for 5min to collect the supernatant;

[0050] Add 30mL pre-cooled ethanol solution (mass percentage concentration is 90%) to the precipitate after centrifugation to continue homogenization (rotating speed is 11000rpm, homogenization time is 8min), centrifugal (centrifugal speed is 4000rpm, centrifugal time is 5min), take Supernatant, so repeated 6 times, combined supernatant.

[0051] Concentrate the combined supernatant in a rotary evaporator until there is no flowing liquid in the obtained concentrate to obtain the orange peel concentrate, which is dissolved in 10 mL of reverse osmosis purified water, and stored at -40°C after s...

Embodiment 3

[0058] (1) The preparation method of tangerine peel extract:

[0059] In 30mL pre-cooled ethanol solution (mass percentage concentration is 100%), add 2.0g dry tangerine peel powder, obtain dispersion liquid; With the rotating speed of 10000rpm, dispersion liquid is homogeneous 10min in high-speed homogenizer; Adopt centrifuge to Centrifuge at a speed of 5000rpm for 3min, and collect the supernatant;

[0060] Add 30mL pre-cooled ethanol solution (mass percentage concentration is 100%) to continue homogenization (rotating speed is 10000rpm, homogenization time is 10min), centrifugation (centrifugal speed is 5000rpm, centrifugal time is 3min) to the precipitate after centrifugation, take Supernatant, so repeated 3 times, combined supernatant.

[0061] Concentrate the combined supernatant in a rotary evaporator until there is no flowing liquid in the obtained concentrate to obtain the orange peel concentrate, which is dissolved in 10 mL of reverse osmosis purified water, and sto...

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Abstract

The invention belongs to the technical field of oral care, and discloses mouthwash containing enzyme and a pericarpium citri reticulatae extract and a preparation method thereof. Per 100mL of mouthwash is prepared from the following components: 2.5-10mL of pericarpium citri reticulatae extract, 0.25-10mg of alpha-dextranase (more than or equal to 50U/mg), 0.25-10mg of beta-dextranase (more than or equal to 50U/mg), 0.25-10mg of alpha-amylase (more than or equal to 50U/mg), 2.5-5mL of peppermint extract, 1g of edible salt and the balance of water. The mouthwash prepared by the method has a remarkable effect on cleaning dental plaque, is capable of effectively inhibiting growth of aerobic and anaerobic bacteria, especially has a remarkable inhibition effect on proliferation of streptococcus, is safe and environment-friendly, and is capable of refreshing breath.

Description

technical field [0001] The invention belongs to the technical field of oral care and relates to a mouthwash, in particular to a mouthwash containing enzyme and tangerine peel extract and a preparation method thereof. Background technique [0002] Dental plaque is a biofilm community formed on teeth by oral flora, especially Streptococcus mutans, which is closely related to dental caries. It is rich in polysaccharides such as glucan and fructan, which can improve the tolerance of bacteria and resist external stress The ability is equivalent to the "hotbed" and "umbrella" for the growth of oral bacteria. Once a large amount of dental plaque is formed, it will help the rapid growth of oral cariogenic bacteria, cause dental caries, gingivitis and other oral diseases, and even increase the risk of other systemic diseases. Most of the current oral rinses focus on the elimination of individual oral bacteria, such as adding lysozyme to sterilize and inhibit bacteria, and pay less a...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61K8/66A61Q11/00
Inventor 陈谷王宏
Owner SOUTH CHINA UNIV OF TECH
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