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Diethylstilbestrol degradation strain and application thereof in sewage and animal manure treatment

A technology of diethylstilbestrol and degrading bacteria, applied in the field of environmental pollution bioremediation, achieving broad industrial prospects, low production costs, and good removal effects

Inactive Publication Date: 2014-12-10
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no patent research and application reports on DES microbial degradation strains at home and abroad.

Method used

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  • Diethylstilbestrol degradation strain and application thereof in sewage and animal manure treatment
  • Diethylstilbestrol degradation strain and application thereof in sewage and animal manure treatment
  • Diethylstilbestrol degradation strain and application thereof in sewage and animal manure treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Bacterial Strains

[0029] Take 5g of activated sludge from a sewage treatment station in Nanjing, add it to 100mL inorganic salt medium, add DES to 50mg L -1 As the sole carbon source, at 30°C, 180r min -1 Cultivate on a shaking table for 7 days, transfer to the same medium with 5% inoculum amount, after three consecutive transfers, dilute the enrichment solution in gradient, take 10 -4 -10 -7 Each 0.1mL of the enrichment solution of the dilution is applied to add 50mgL -1 On the inorganic salt plate of DES, after cultivating at 30°C for 4 days, pick the single colony that grew out and inoculate it in a medium containing 50 mg L -1 In the inorganic salt liquid medium of DES, at 30°C, 180r min -1 Incubate on a shaking table for 7 days to verify its degradation effect on DES. The formula of inorganic salt medium is: NH 4 NO 3 1.0g·L -1 , KH 2 PO 4 0.5g·L -1 , K 2 HPO 4 1.5g·L -1 , NaCl 1.0g·L -1 , MgSO 4 ·7H 2 O ...

Embodiment 2

[0036] Example 2 Fermentation and immobilization of the restoration bacterial agent

[0037] The process of using the above-mentioned strain S to produce the restoration bacterial agent is as follows: slant seed—shake flask seed solution—seed tank—immobilized product (the packaging dosage form is immobilized bacterial agent).

[0038] 1) Inoculate the Serratia sp. S test tube species with the preservation number CGMCC 1.12941 in the fermentation medium, and shake and cultivate to the logarithmic phase;

[0039] 2) Insert the inoculum amount of the above-mentioned cultured strains into 70% (V / V, based on the volume of the fermenter, based on the volume of the fermenter, The same below), cultivated to the logarithmic phase;

[0040] 3) The seed liquid is inserted into a production tank with a liquid filling capacity of 70% according to 10% inoculum amount for cultivation;

[0041] 4) During the cultivation process of seed tanks and production tanks, the ventilation rate of ste...

Embodiment 3

[0044] Example 3 Biodegradation Experiment of DES by Bacterial Strain S in Medium

[0045] In the inorganic salt culture medium (with embodiment 1), adding final concentration is 50mg L -1 DES was inoculated into strain S (CGMCC 1.12941) with a 5% (V / V, based on the medium volume) inoculation amount, and inoculated with inactivated strain S (CGMCC 1.12941) as a control, and cultured in a constant temperature shaker at 30°C. Regularly sampled to detect strain S (CGMCC 1.12941) with 50mg L -1 The growth of DES as the only carbon source and the degradation of DES, the results are shown in figure 2 . Strain S (CGMCC 1.12941) can grow well with DES as the sole carbon source, and can respond to an initial concentration of 50 mg L within 7 days -1 The degradation rate of DES reaches more than 65%.

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Abstract

The invention discloses a diethylstilbestrol degradation strain and an application thereof in sewage and animal manure treatment. Serratieae (Serratia sp.) S is preserved at China General Microbiological Culture Collection Center on August 26, 2014, wherein the strain preservation number is CGMCC 1.12941. The invention also discloses an application of the serratieae S and a fungicide of the serratieae S in degradation of estrogen, especially degradation of diethylstilbestrol. The serratieae S is obtained in a screening manner, the degradation rate on DES (diethylstilbestrol) of which the initial concentration is 50mg L<-1> can reach over 80% after an immobilized bacteria agent which is prepared from the strain is subjected to shake-flask culture in a laboratory for 7d, the immobilized bacteria agent is directly applied to DES-polluted water and animal manure, and the DES residue within a short period of time is reduced by over 90%, so that the problem of hazards of diethylstilbestrol DES to the water environment, animal manure and human health is solved.

Description

technical field [0001] The invention belongs to the field of bioremediation of environmental pollution, and relates to a diethylstilbestrol-degrading bacterial strain and its application in the treatment of sewage and livestock manure. Background technique [0002] Environmental estrogen has become the third largest environmental problem after the destruction of the ozone layer and global warming, and has been hailed as a time bomb that threatens the survival of human beings. In recent years, various levels of estrogen pollution have been found in many water bodies around the world, which have had adverse effects on humans and animals. In my country's Yangtze River, the Yellow River Basin also has the detection of estrogen. When the concentration of estrogen in water is 1ng·L -1 Sometimes it will interfere with the endocrine system of fish, resulting in the feminization of male fish. The normal physiological secretion and excretion of humans and animals will release estro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C02F3/34A62D3/02C12R1/425C02F101/38A62D101/28
Inventor 刘娟孙敏霞凌婉婷
Owner NANJING AGRICULTURAL UNIVERSITY
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