Portable probing type surface plasma resonance biosensor and preparation method and detection method thereof

A surface plasmon and biosensor technology, which is applied in the measurement of scattering characteristics, etc., can solve the problems of weak coupling light, complex preparation process, small core diameter of single-mode optical fiber, etc., and achieve the effect of low cost and simple preparation process

Inactive Publication Date: 2014-12-10
XI AN JIAOTONG UNIV
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing problems of fiber optic SPR sensors are: the sensitivity and signal-to-noise ratio of multimode fiber SPR are not high, and the resonance wavelength is dispersive; while the core diameter of single-mode fiber is small, the coupling light is weak
In addition, the preparation process of fiber optic SPR sensing probes is more complicated than that of planar sensor sheets.
In order to overcome the above problems, sensor probes processed with optical glass rods instead of optical fibers appeared (Takuo Akimoto, Analytica Chimica Acta 610(2008) 119–124), but beam splitters are required, and the reflected light is less than half One of the light intensity enters the spectrometer, and the signal is lost by more than half

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Portable probing type surface plasma resonance biosensor and preparation method and detection method thereof
  • Portable probing type surface plasma resonance biosensor and preparation method and detection method thereof
  • Portable probing type surface plasma resonance biosensor and preparation method and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] The preparation method of the penetrating surface plasmon resonance biosensor 2 of the present invention comprises the following steps:

[0037] 1) Cut K9 or B270 optical glass into strip optical glass, the geometric size is 60mm×4mm×2mm, then polish the strip optical glass into an optical plane, and then use a vacuum coating machine to coat the bottom of the strip optical glass A metal internal reflection film 7 with a thickness of 200-250nm; then a chromium film with a thickness of 2nm is plated on the opposite side walls of the strip optical glass bottom, and a gold film with a thickness of 45-50nm is plated on the chromium film;

[0038] 2) Immerse the part of the strip optical glass coated with gold film in a solution composed of concentrated sulfuric acid and hydrogen peroxide with a volume ratio of 3:1 for surface treatment, rinse it with a large amount of deionized water and dry it with nitrogen after taking it out; and the concentrated sulfuric acid The mass co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention relates to a portable probing type surface plasma resonance biosensor and a preparation method and a detection method of the portable probing type surface plasma resonance biosensor. The sensor comprises strip optical glass, an inner meal reflecting film coats the bottom of the strip optical glass, a chromium film coats the side wall of the strip optical glass, a gold film coated with a biomolecular sensitive layer coats the nano chromium film, a collimator is arranged on an incident light path of the strip optical glass, and a polarizing film, an optical fiber collimator and an optical fiber spectrometer connected with the optical fiber collimator by a multimode optical fiber are sequentially arranged on an emergent light path. The inner meal reflecting film coats the bottom end of the strip optical glass, the chromium film and the gold film sequentially coat the side wall of the top part of the strip optical glass, the biomolecular sensitive layer is formed on the surface of the gold film, and BSA solution is used for sealing to obtain the probing type surface plasma resonance biosensor. The portable probing type surface plasma resonance biosensor is inserted into liquid in a test tube for SPR measurement, the system is simplified, and the portability of the sensor is achieved; and besides, the portable probing type surface plasma resonance biosensor overcomes the disadvantages of the existing probing type SPR sensor, the measurement mode is simple and the portability of the instrument is realized.

Description

technical field [0001] The invention relates to a surface plasmon resonance biosensor, in particular to a portable penetrating surface plasmon resonance biosensor and a preparation and detection method thereof. Background technique [0002] Surface plasmon resonance (SPR) is a physical optical phenomenon produced by the coupling of light beams through the surface of a medium and metal thin films. In 1902, Wood first discovered this phenomenon in optical experiments. One of the necessary conditions for the formation of SPR is the existence of the interface between the metal and the dielectric. When the parallel light on the parallel surface is incident at an angle greater than the critical angle of total reflection, if its frequency is consistent with the frequency of free electrons (ie, plasma) oscillating on the metal surface, The incident light will be coupled into the surface plasmon. At this angle, the surface plasmon resonance will cause significant absorption of the e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/55
Inventor 方湘怡李俊锋杨玉琮吴逸川姚曼文王林玉梁晓慧
Owner XI AN JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products