Purple scallop ovum and bay scallop sperm hybrid scallop maintainer line construction method and application

A method for constructing a maintainer line, which is applied in the field of shellfish breeding technology--cultivation of commercial seedlings of hybrid scallops, can solve the problems of inability to establish a maintainer line and a restorer line of male sterile lines, and achieve the effect of overcoming the obstacles of self-fertilization

Active Publication Date: 2014-12-17
烟台海之春水产种业科技有限公司
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so far, so it is impossible to establish male sterile lines similar to crops and their maintainer and restorer lines for hybrid breeding

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Screening of male sterile individuals of Zihai hybrid scallops and breeding of their male parents: 50 purple scallops with mature gonads and 50 bay scallops were selected in the first year, and each scallop was placed in the pre-added scallop after 30 minutes of stimulation in the shade. Spawn eggs and ejaculate in a separate container in seawater at 23°C, collect the eggs of each purple scallop and the sperm and eggs of each bay scallop separately, add a small amount of eggs from a single bay scallop to the egg suspension of each purple scallop Stir to mix the sperm and eggs evenly, observe under a microscope so that there are 5-6 sperm around each egg, and place the fertilized eggs in a dark environment to incubate to obtain the first-generation family of Zihai hybrids. Use the above method to establish at least 20 such hybrid families, and at the same time, mix and fertilize the fertilized bay scallop sperm eggs to establish a corresponding number of bay scallop se...

Embodiment 2

[0025] At the end of March 2008, the purple scallops with mature gonads were taken, and the eggs of the purple scallops were obtained after temperature stimulation. In the spring of 2009, the scallops with large individuals and only developed in the female area were selected from the above-mentioned hybrid scallops after maturation. After drying in the shade for 30 minutes, the scallops were placed in a container filled with seawater at 23°C to stimulate spawning. The sperm of the scallops were mixed and fertilized to create a backcross population of the offspring of the purple sea hybrid scallops and the bay scallops. The backcross population was mostly hermaphroditic after maturation in spring 2010, but <10% of individuals still showed male sterility.

[0026] Continue to backcross the male sterile individuals with the sperm of the self-bred progeny of their male parent bay scallops, and after one or more generations of selective breeding, a male sterile line (female parent)...

Embodiment 3

[0028] In the spring of 2010, male-sterile individuals in the offspring of the hybrid offspring of purple scallop eggs and bay scallop sperm established in 2009 were selected, stimulated to lay eggs after maturation, and fertilized with bay scallop sperm, establishing 12 backcross families and raising them. to make. After maturation in the spring of 2011, it was found that most of the individuals in 11 families were hermaphrodites, while in one family, most individuals developed only the female area, and a few individuals did not develop gonads.

[0029] According to this method, the eggs of a single male sterile individual were backcrossed with the sperm of a single bay scallop to establish several backcross families, and the sperm donor bay scallop was selfed to establish corresponding inbred families. In the second year, check the gonad development of the backcross family. If there are male infertile individuals in the backcross family, the fertilization ability of the eggs...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a purple scallop ovum and bay scallop sperm hybrid scallop maintainer line construction method and application. According to a maintainer line, a corresponding purple scallop ovum and bay scallop sperm hybrid scallop male sterility line offspring is a bay scallop strain which is in male sterility and ovum fertility, and the purple scallop ovum and bay scallop sperm hybrid scallop is the hybrid offspring of the purple scallop ovum and the bay scallop sperm. The purple scallop ovum and bay scallop sperm hybrid scallop maintainer line construction method comprises the following steps of firstly, constructing a great quantity of first filial generation families of the purple scallop ovum and bay scallop sperm hybrid scallop, and finding a male sterility individual for breeding a male sterility line from the first filial generation families; then, continuously backcrossing by the male parent bay scallop sperm to respectively breed a male sterility line-maintainer line combination of which the offspring is completely the male sterility individual; finally, hybridizing to produce male sterility line parent scallop by the male sterility line and the maintainer line, wherein the male sterility line parent scallop is used for producing the offspring seed of the purple scallop ovum and bay scallop sperm hybrid scallop product. According to the invention, the scallop maintainer line is established in an animal for the first time, and therefore, autofertilization obstacles in hermaphroditic scallop hybrid are effectively overcome.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to the construction and application of a maintainer line of Zihai hybrid scallop (hybrid offspring of purple scallop ova and bay scallop sperm). Background technique [0002] The bay scallop (Argopecten irradians irradians) is native to the Atlantic coast of the United States and was introduced to my country in 1982. The bay scallop grows fast, has a wide range of temperature adaptability, and has strong stress resistance. It is very suitable for farming in the northern seas of my country. At present, the output and farming area account for more than 50% of the scallop farming in my country. However, due to the long-term inbreeding after the introduction, the germplasm of bay scallops has been seriously degraded during the breeding process. The size of commercial shellfish is getting smaller and smaller, and the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王春德孙奎府马斌
Owner 烟台海之春水产种业科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap