Instrument-free quantitative analysis method for fast detecting test paper, and application of instrument-free quantitative analysis method

Abstract

The invention discloses an instrument-free quantitative analysis method for fast detecting test paper, and application of the instrument-free quantitative analysis method. A target analyte or a secondary analyte in a sample can react with a detection reagent to generate insoluble deposits to enhance the hydrophobicity of a reaction area, and the concentration of the analyte is in direct proportion to the enhancement degree of the hydrophobicity of the reaction area; the hydrophobicity of the reaction area decides the volume of a colored hydrophilic reagent solution flowing into a quantification area from the reaction area within set time or in a set distance (such as from the tail end of the reaction area to the quantification area), and the length of colored print in the quantification area is in direct proportion to the volume of the colored reagent solution flowing into the quantification area and is in inverse proportion to the hydrophobicity of the reaction area. Therefore, the concentration of the analyte in the sample is in inverse proportion to the length of the colored print in the quantification area, and the fast quantitative detection of the analyte in the sample can be carried out by measuring the length of the colored print in the quantification area through a ruler with scales. The method can be applied to quantitative detection on target analytes in various liquid samples of urine, blood, serum, saliva, water, etc.

Description

technical field [0001] The invention belongs to the technical field of rapid detection test paper product development and its application, in particular to an instrument-free quantitative analysis method for rapid detection test paper and its application. Background technique [0002] As a simple and low-cost analysis tool, rapid test strips have been widely used in all aspects of real life, such as pregnancy test strips, urine test strips, pH test strips, nitrite test strips, pesticide residue test strips, etc. However, except for colloidal gold immunochromatographic test strips and household blood glucose meters, most of the traditional rapid test strips can only achieve semi-quantitative or qualitative analysis of analytes in samples, which greatly limits their practical applications. In recent years, new rapid detection test strips and related quantitative analysis methods have been continuously reported. The test paper in the present invention belongs to a new type of ...

AI Technical Summary

Problems solved by technology

Although these methods can quickly detect the quantitative analysis of test strips, the professional analytical instruments involved (such as electrochemical workstations, fluorescence analyzers, chemical analyzers, etc.) are bulky and expensive, thus significantly increasing the analysis cost, while operating steps Relatively cumbersome and time-consuming, and requires high professional skills for operators, especially cannot meet the increasing requirements for real-time inspection and on-site analysis, and is difficult to be widely used in various fields

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