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A method to optimize the cryopreservation effect of vitrification of lily embryogenic callus

An embryogenic callus, vitrification ultra-low temperature technology, applied in horticultural methods, plant preservation, botanical equipment and methods, etc., can solve problems such as the establishment of an ultra-low temperature preservation system for lily embryogenic callus, and achieve preservation effects Significantly, improve the preservation effect, restore the effect of increasing the growth rate

Active Publication Date: 2016-04-13
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the cryopreservation of lily embryogenic callus as material, and the cryopreservation system of lily embryogenic callus has not been established yet.

Method used

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  • A method to optimize the cryopreservation effect of vitrification of lily embryogenic callus
  • A method to optimize the cryopreservation effect of vitrification of lily embryogenic callus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0051] 1) The lily embryogenic callus subcultured for 20 days was pre-cultured at 4° C. for 2 days on MS solid medium containing 0.7 mol / L sucrose;

[0052] 2) Transfer to the loading solution for soaking at room temperature for 60 minutes;

[0053] 3) Transfer to the vitrification solution and dehydrate at 0°C for 60 minutes;

[0054] 4) Finally, store in liquid nitrogen at ultra-low temperature.

[0055] After step 3), without removing the vitrification solution, directly place the lily embryogenic callus soaked in the vitrification solution in liquid nitrogen for cryopreservation.

[0056] According to the above steps, the embryogenic calli of lily were divided into experimental group and control group.

[0057] Among them, the vitrification solutions of the experimental groups contained graphene quantum dots of 0.1 g / L, 0.3 g / L, and 0.5 g / L respectively.

[0058] Specifically, the vitrification solution of experimental group 1 contained 0.1g / L graphene quantum dots; the...

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Abstract

The invention discloses a method for optimizing a vitrification ultra-low temperature preservation effect of lily embryonic callus. According to the invention, the lily embryonic callus is processed by adopting a vitrification solution which contains a carbon nanometer material so as to improve the preservation effect of the lily embryonic callus. The method specifically comprises the steps of preculture, loading liquid treatment, vitrification solution treatment and liquid nitrogen preservation, wherein the vitrification solution contains 0.1-0.5 g / L of grapheme quantum dots. The method disclosed by the invention optimizes the preservation effect of the lily embryonic callus remarkably; as the grapheme quantum dots are added to serve as an allogenic material, the vitrification ultra-low temperature preservation of plants is facilitated.

Description

technical field [0001] The invention relates to the field of preservation of plants or parts thereof, in particular to a method for optimizing the ultralow temperature preservation effect of lily embryogenic callus vitrification. Background technique [0002] Cryopreservation is a modern in vitro preservation technology for germplasm resources developed in the 1970s. Usually stored in liquid nitrogen, the substance metabolism and growth activities in the preserved material cells are almost completely stopped, and they are in a relatively stable biological state, achieving the purpose of long-term preservation of germplasm. Ultra-low temperature preservation is currently the only method that does not require continuous subculture. Medium and long-term preservation method. Vitrification cryopreservation is to place cells or tissues in a vitrification solution composed of a certain proportion of permeable and non-permeable protective agents, so that the material and its vitrif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00A01H4/00
Inventor 张荻任丽陈冠群申晓辉
Owner SHANGHAI JIAOTONG UNIV