A fixed observation device for live imaging of animal spinal cord

Abstract

A fixing and observing device for living imaging of animal spinal cords comprises a bottom plate (1), a left movement adjusting device (2), a right movement adjusting device (3) and an embedded observation window (4). The left movement adjusting device (2) and the right movement adjusting device (3) are fixedly connected to the bottom plate (1) through screws. The embedded observation window (4) is in insertion connection with rectangular protrusions of horizontal positioning sliders on the left movement adjusting device (2) and the right movement adjusting device (3) through rectangular holes in V-shaped frames to be positioned. By the fixing and observing device, operations for installing the embedded observation window can be facilitated to improve stability of the spinal cords, and dynamic observation of post-operation two-photon spinal-cord imaging can be also facilitated. The horizontal positioning sliders on the left movement adjusting device and the right movement adjusting device can guarantee horizontal stable internal fixation states for implant operations and can further provide stable external fixation support during post-operation observation.

Description

Technical field: [0001] The invention relates to a fixed observation device for living animal spinal cord imaging, which is mostly used in experimental research in the field of neurobiology, can perform implanted observation window installation operations on animals, and can perform long-term dynamic observation of spinal cord nerve tissue and its components in vivo. Changes in blood vessels are of great value in the study of the central nervous system, especially the physiological and pathological changes of spinal cord injury, as well as the regeneration and repair of nerves after spinal cord injury. Background technique: [0002] In the experimental research in the field of neurobiology, the traditional method of in vivo imaging of the animal spinal cord is to excise a segment of the lamina to expose the spinal cord, use the whole body suspension method or local clamping to stabilize the spine, and then use the fluorescence microscope or two-photon Microscopy for in vivo ...

AI Technical Summary

Problems solved by technology

However, this process cannot last for a long time. Prolonged anesthesia and repeated operations will not only harm the animal's systemic function, but also increase the risk of infection, increase the inflammatory response, cause tissue damage, and cause more pain to the animal. Not conducive to further observation and experimental research

Moreover, there is currently no uniform standard for surgical fixation and postoperative observation of external fixators, resulting in complicated surgical operations and difficulties in postoperative imaging and fixation.

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