68 results about "Intravital Imaging" patented technology

The invention discloses an application of pegylated irinotecan in preparation of a medicine for preventing and / or treating triple-negative breast cancer brain metastasis diseases, which is shown by in-vivo imaging observation and lifetime observation of high, medium and low dose groups of pegylated irinotecan with a specific structure. The high-dose group, the medium-dose group and the low-dose group all have a definite anti-tumor effect, bioluminescence signal values are inhibited to different degrees, the lifetime is prolonged, and the lifetime of the high-dose group and the lifetime of the medium-dose group are remarkably longer than that of the NKTR-102 group.

The application provides a chemical luminescence substrate with high chemical luminescence intensity, long wavelength and good stability, and a preparation method and application thereof. The chemical luminescence substrate has a structure shown in formula I. In addition, the application provides spontaneous fluorescence wavelength and intensity of the chemical luminescence probe in a physiological environment, and application of the chemical luminescence probe in in-vivo imaging. Test results show that the chemical luminescence probe provided in the application has long-wavelength spontaneous fluorescence (600 nm), can effectively penetrate skin tissue, has high spontaneous fluorescence intensity (> 10 7 p / s / cm 2 / sr), excellent thermal stability (without 1,2-dioxetane structure) and diversified detection groups (suitable for different detection models).

The invention belongs to the technical field of nano biological materials, and particularly relates to a near-infrared two-region excitation and emission lanthanide nano fluorescent probe as well as a preparation method and application thereof. Lanthanide ions with near-infrared two-region absorption characteristics are used as sensitizers, nanocrystalline with a co-doped structure formed by the lanthanide ions and activator ions is used as a core, and a pure-phase matrix material shell layer which is the same as a core lattice is grown on the surface of a core particle in situ. The nano fluorescent probe can generate tunable near-infrared two-region fluorescence emission with the wavelength of 1000 to 3000 nm under the excitation of near-infrared light with the wavelength of more than 1000 nm. The near-infrared two-region excitation and emission characteristics of the nano fluorescent probe can provide higher tissue penetration depth and signal-to-noise ratio for in-vivo imaging. The nano fluorescent probe shows excellent performance in living body angiography, dynamic observation of intestinal tracts and multi-channel near-infrared two-region imaging, and has wide application prospects in the fields of biomedical detection, early diagnosis of diseases, surgical navigation and the like.

The invention belongs to the technical field of GPCR drug research and development, and discloses a ratio type cAMP fluorescent probe RaCamp and a construction method of a transgenic mouse, the probe RaCamp is formed by series fusion of G-Flamp2 fluorescent protein and mCherry fluorescent protein through a flexible linker, and the preparation method of the probe RaCamp comprises the following steps: S1, obtaining an mCherry fragment; s2, a G-Flamp2 linear carrier is obtained; s3, homologous recombination; s4, converting and screening; the construction method of the transgenic mouse comprises the following steps: T1, designing a carrier; t2, microinjection of fertilized eggs; t3, embryo transplantation and reproduction; the invention has the beneficial effects that mCherry is added as an internal reference protein on the premise of keeping the sensitivity of the original G-Flamp2 probe, and the selected connecting peptide (GGGGS) 2 enables the expression quantity of the two proteins in cells to be accurately controlled to be 1: 1, so that the problem of background interference in cell observation of in-vivo imaging is well solved. And the sequence is inserted into a Rosa26-LSL box, so that the construction of a Rosa26-LSL-RaCamp mouse is realized.

The invention relates to the technical field of biology, in particular to a cryopreserved stem cell diluent and application thereof.The cryopreserved stem cell diluent further comprises cysteine and polyvinylpyrrolidone besides a compound electrolyte injection, the concentration of cysteine in the cryopreserved stem cell diluent is 1-10 mM, and the concentration of polyvinylpyrrolidone in the cryopreserved stem cell diluent is 1-10 mM. The concentration of the polyvinylpyrrolidone in the cryopreserved stem cell diluent is 0.02 to 0.1 g / mL. The cryopreserved stem cell diluent can improve the cell activity and cell distribution after the cryopreserved cell preparation is recovered, and can monitor the application of cells in rat living body imaging.

The application belongs to the technical field of fluorescent probe, and particularly relates to an aminopeptidase fluorescent probe and a preparation method and application thereof. In order to develop an APN near-infrared fluorescent probe suitable for in vivo imaging, a center-position ester group substituted pentamethine cyanine dye platform (Cy5-COOM) is used, and based on an "ester group to carboxyl group" conversion strategy, an aminopeptidase (APN) fluorescent probe Cy5-APN is developed. The probe is firstly hydrolyzed by APN, then a 1,6-elimination reaction occurs, and finally a strong fluorescent center-position carboxyl group substituted pentamethine cyanine dye Cy5-COO is released. In addition, in view of the characteristics that cancer cell surfaces overexpress APN, the probe can distinguish cancer cells / tissues and normal cells / tissues with high contrast, and therefore has great application potential in fluorescence-guided tumor surgery.

The application provides a method for improving the precision of blood vessel information extraction in OCTA live imaging, comprising the following steps: performing two-dimensional wavelet decomposition on an image to obtain a low-pass frequency component, a horizontal structure detail component, a vertical structure detail component and a diagonal structure detail component; selecting the vertical structure detail component to perform fast Fourier transform to obtain a frequency spectrum diagram of the vertical structure detail component; performing frequency domain filtering on the frequency spectrum diagram of the vertical structure detail component by using a band-pass filter to obtain a filtered frequency domain signal; performing inverse fast Fourier transform on the filtered frequency domain signal and wavelet inverse transform to reconstruct a filtered image; comparing each pixel point in the reconstructed filtered image and the original image and taking the minimum value; iteratively performing the above process multiple times to reconstruct a deep denoising image; and the application can effectively remove the stripe motion artifacts existing in the OCTA live imaging result, and has very good reservation capability for image quality and blood vessel information.

The utility model relates to the technical field of plant living body imaging, and discloses a plant living body imaging and simulation illumination device which comprises a box body, when the device is used, an LED plant growth lamp can be turned on firstly through an arranged environment simulation assembly, and light irradiates a plant through a through hole; light is dispersed around plants through the astigmatic lenses arranged on the through holes, so that more real natural light is simulated, meanwhile, natural light such as sunny days, cloudy days and night with moonlight can be simulated through the colored lenses, and an environment closer to the natural environment is created. According to the present invention, with the arrangement of the CCD camera, the weak fluorescence signal can be captured, the specific gene of the living plant can be subjected to fluorescence labeling, and after the EMS mutagenesis treatment, the fluorescence brightness change can be detected in the imaging device so as to find the enhancer or the suppressor, such that the biological research data can be obtained; and a better use prospect can be brought.

1. The name of the design product: animal live imaging instrument. 2. The use of the design product: for live small animal imaging. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

1. Name of the product in this design: Live Imaging Organ Fixation Instrument. 2. Purpose of this design: To fix organs or tissues of live experimental animals during microscopic imaging. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The invention discloses a formaldehyde probe as well as a preparation method and application thereof, and belongs to the technical field of fluorescent probe preparation. Dissolving benzylamine and potassium carbonate in anhydrous acetonitrile, adding 2-bromo-2-methyl propionate, and reacting to obtain a compound 3; dissolving the compound 3 and lithium hydroxide in a mixed solution of tetrahydrofuran and water, reacting for a period of time, and quenching the reaction with hydrochloric acid to obtain a carboxylic acid intermediate 4; and dissolving the carboxylic acid intermediate 4 in dichloromethane, then adding CDM, EDCI and DMAP, and carrying out a reaction to obtain the target product DPM. The DPM is a formaldehyde regeneration type fluorescent probe and can realize formaldehyde detection in a mode of disturbing an endogenous steady state to the minimum extent. The DPM is a steady-state maintenance type formaldehyde probe successfully applied to a spinal cord injury (SCI) model for the first time, and can realize visual monitoring on formaldehyde rise of a diseased region based on a non-invasive living imaging system (IVIS).

The application discloses an electron acceptor, a fluoroborondiphenylamine fluorescent dye and a preparation method and application thereof, wherein, the difluoroboron bridged difluoroboron pseudoindole electron acceptor is easy to synthesize, and can be further condensed with a Fischer aldehyde or a derivative thereof to obtain a series of fluoroborondiphenylamine fluorescent dyes covering far infrared to near infrared, which effectively enriches the types of fluorescent dyes, and the application further provides the fluoroborondiphenylamine fluorescent dye which can be used for lipid droplet fluorescent imaging, and the effects of high-efficiency lipid droplet targeting, high signal-to-noise ratio lipid droplet imaging and multi-color (multi-channel) fluorescent lipid droplet imaging are achieved, wherein, the near-infrared lipid droplet probe has better application prospect in tissue and living body imaging.

This utility model discloses a large-spot laser application device, including a device box, and an angle adjustment plate, a laser light source, and a projection plate disposed inside the device box. The two angle adjustment plates are respectively disposed on both sides above the projection plate, and a laser light source is installed on each of the two angle adjustment plates. The irradiation direction of the laser light source is aligned with the projection plate, and the laser light source and the angle adjustment plate are connected to form an adjustable irradiation angle structure. This utility model uses a laser light source to replace the traditional LED light source, halogen light source, and xenon lamp light source, which not only extends the service life of the light source, but also makes the light source smaller and more conducive to the integration of the entire structure. It can be directly applied to the excitation of light sources in CCD camera multicolor fluorescence chemiluminescence gel imaging systems and animal and plant live imaging systems without replacing the light source.

This application discloses an optical in vivo imaging device and method for animal spleen, belonging to the field of biomedical devices and instruments. The imaging device includes a first imaging block and a second imaging block; the first imaging block is provided with a first notch recessed inward from its end face, and the top view projection of the first notch includes a first arc; the second imaging block is provided with a second notch recessed inward from its end face, and the top view projection of the second notch includes a first straight line, a second arc, and a second straight line connected in sequence; the first imaging block is fitted and snapped into the second imaging block so that the first and second notches form a closed imaging window with an adjustable imaging area. This application has a large effective imaging area, enabling full-field, highly stable imaging of the spleen, and 100% observation of all microstructures of the spleen. It can image the marginal areas and white pulp areas located in deeper positions, and can achieve cross-regional tracking observation.

This invention relates to a medaka hatching enzyme, its preparation method, and its application. The preparation method of the medaka hatching enzyme includes the following steps: 1) collecting fertilized medaka eggs; 2) incubating the fertilized medaka eggs with embryo culture medium in a 26℃ incubator for 6 days, and collecting mature embryos; 3) adding NaCl solution and Tris-HCl buffer (pH=8.0) to the mature embryos, grinding them, collecting the filtrate, and allowing it to stand overnight at 4℃; 4) centrifuging the filtrate at 15000 rpm for 15 minutes, and extracting the supernatant to obtain the medaka hatching enzyme solution. The preparation method is simple to operate, and the obtained medaka hatching enzyme has high activity, which can help to quickly remove the medaka eggshell artificially. The naked medaka embryos obtained by this method can be directly used for in vivo imaging and in-situ quantitative analysis, and embryo model construction, providing support for subsequent genetic, developmental biological, and toxicological studies of medaka embryos.

This application provides a ratiometric fluorescent probe for detecting divalent copper ions, its preparation method, and its application. The ratiometric fluorescent probe comprises fluorescently labeled human serum albumin with isothiocyanate and a dye compound; the fluorescently labeled human serum albumin with isothiocyanate is coated on the surface of the dye compound; the dye compound is a compound with the chemical formula shown in Formula I, II, III, or IV. The ratiometric fluorescent probe provided in this application can be applied to various Cu... 2+ The detection scenario, and can detect Cu 2+ Quantitative detection, on-site rapid testing, and live imaging analysis are performed.

Providing imaging equipment 2 capable of obtaining high-precision images within living organisms. [Solution] The imaging device 2 has a subshaft 6, a first cable 8a, a second cable 8b, and a head 10. The head 10 has a cylinder 12, a filler 14, a cantilever 31, a guide 18, a camshaft 20, and a cam 22. The cantilever 31 includes a first sensor 30a and a second sensor 30b. The guide 18 has a guide groove 32. The cantilever 31 is inserted into this guide groove 32. The rotation of the subshaft 6 causes the cam 22 to rotate. The rotation of the cam 22 causes the cantilever 31 to reciprocate while being guided by the guide groove 32.

The present application relates to the technical field of imaging, in particular to an optical coherence elastography method and device, electronic equipment and storage medium, wherein the method comprises: outputting laser light meeting preset time periodicity conditions and preset linear variation conditions by using a sweep frequency light source; generating a driving signal containing an analog signal or a digital signal according to a trigger signal; scanning a sample according to the driving signal; applying mechanical excitation to the sample by using the driving signal, generating two-dimensional imaging and three-dimensional imaging of the sample according to two-dimensional elastic waves and three-dimensional elastic waves respectively; interfering laser light reflected by a sample arm with reference arm laser light; reconstructing the interference signal into a sample structure image, generating biomechanical information of the sample according to the elastic waves, and determining the final optical coherence elastography result according to the biomechanical information, the three-dimensional imaging and the two-dimensional imaging. Thus, the problem that the existing optical coherence elastography method cannot realize two-dimensional dynamic imaging and three-dimensional in vivo imaging is solved.

The invention relates to the technical field of supramolecular chemistry, in particular to a tetradentate pyridine ligand, a near-infrared two-region fluorescent metal cage as well as a preparation method and application of the tetradentate pyridine ligand and the near-infrared two-region fluorescent metal cage. The structural formula of the tetradentate pyridine ligand is shown in the specification, R is shown in the specification, and the element X is S or Se. The tetradentate pyridine ligand disclosed by the invention is a typical tetradentate pyridine ligand with a D-pi-A-pi-D structure and has strong near-infrared absorption capacity and efficient near-infrared two-region emission, and absorbed photon energy can be released in a fluorescent form; therefore, the metal cage prepared from the metal nano-material can be applied to in-vivo near-infrared two-region fluorescence imaging of mouse tumors. Due to the small ground state-excited state energy gap difference of the tetradentate pyridine ligand, photon energy absorbed by the metal cage prepared from the tetradentate pyridine ligand can also be released in the form of non-radiative transition, namely heat, so that the metal cage can be further applied to photo-thermal imaging and photo-thermal treatment and can play a complementary role with near-infrared two-region fluorescence imaging, and the in-vivo imaging quality is improved.

The utility model relates to observation device technical field belongs to a kind of mouse live imaging fixing device based on inverted microscope, including fixed on the fixed device box base body and base of microscope platform, the fixed device box base body bottom is fixedly connected on base, the upper portion of fixed device box base body is equipped with detachable seal cover, the base is opened on the observation window, the fixed device box base body perimeter is also opened experimental fixed hole. The utility model can flexibly match the tissue of surgical exposure mouse and the mouse of surgical installation observation window are observed using inverted microscope, reduce the development cost of multiple sets of fixed device, can also achieve certain mouse physiological state maintenance function, and compatible various equipment required by experiment, simplify live imaging observation procedure.

The invention provides an X-ray plate microscopic imaging loading device for a living embryo sample and a long-term microscopic imaging method, and belongs to the technical field of X-ray plate microscopic imaging. The loading device mainly comprises an FEP cabin body, a fixing ring, a sealing gasket, a sealing cover, a liquid adding capillary tube, a liquid pumping capillary tube, a gas supply capillary tube and a pressure balance capillary tube. The FEP cabin body is prepared through a forming device. The loading device disclosed by the invention can realize rapid and lossless loading of small samples such as embryo-like samples; the closed design can provide a sterile environment; the liquid adding / pumping capillary tube is matched with the injection pump, and the gas supply / pressure balance capillary tube is matched with the gas controller, so that the replacement of a culture solution and the maintenance of a gas environment required by the living embryo sample culture can be automatically realized, and the normal growth and development of the sample in the X-ray plate microimaging process are ensured. The loading device can be matched with different light-sheet microscopic imaging systems, so that uninterrupted high-resolution microscopic imaging observation of the light-sheet microscope in the living embryo sample development process is realized.

A recombinant lentiviral vector comprising a coding sequence for an OR protein fused to a coding sequence for a fluorescent protein or a subunit of a fluorescent protein, and a promoter active in human cells operatively linked to the coding sequences. A recombinant lentivirus comprising a recombinant genome comprising an RNA that generates an ANCH sequence upon retrotranscription. A recombinant eukaryotic cell comprising a genomically integrated DNA copy of the recombinant lentiviral vector. A method of observing lentiviral DNA in a eukaryotic cell, comprising: providing a recombinant eukaryotic cell that produces a fusion protein comprising an OR protein, fused to a fluorescent protein or a subunit of a fluorescent protein; infecting the recombinant eukaryotic cell with a recombinant lentivirus comprising a recombinant genome comprising an RNA that generates an ANCH sequence upon retro-transcription, under conditions sufficient for reverse transcription of the recombinant lentiviral genome comprising an ANCH sequence; allowing the OR protein to bind to the ANCH sequence; and detecting the fluorescent protein or subunit of the fluorescent protein to thereby observe the lentiviral DNA in the eukaryotic cell. This tool can be suitable also for in vivo applications (e.g. humanized mice) as well as for screening of new antiretroviral compounds. The HIV-1 ANCHOR system can be extended to the study of other viruses or for the screening of antiviral compounds, e.g. against SARS-CoV2.

The utility model relates to the technical field of living body imaging, and discloses a door lock device with a living body imaging hinge, which comprises a door frame, a plurality of rotating mechanisms are fixedly connected to the right side of the inside of the door frame, a door body is rotatably connected to the inside of the door frame, and a door lock body is fixedly connected to the outside of the door body. Fixing mechanisms are arranged at the upper end and the lower end of the exterior of the door lock body; the multiple rotating mechanisms comprise bases, mounting frames are fixedly connected to the outer portions of the bases, through holes are formed in the outer portions of the mounting frames, connecting rods are slidably connected to the interiors of the through holes, pressing plates are rotatably connected to the outer portions of the connecting rods, and sliding grooves are formed in the outer portions of the mounting frames. According to the utility model, the connecting rod smoothly slides in the through hole and is connected to the pressing plate, so that an operator can conveniently apply force. The elastic assembly such as the first spring provides elastic restoring force for the rotating mechanism, and smoothness and stability of rotation are guaranteed.

This invention relates to a class of dihydroxanthine chemiluminescent groups, their preparation methods, and applications. The dihydroxanthine chemiluminescent group developed in this invention, compared to the traditional phenolic dioxane chemiluminescent group which uses two rigid six-membered rings to fix the double bond conformation, exhibits a higher luminescence quantum yield and a longer emission wavelength. More importantly, it possesses a reactive aldehyde group, which, while maintaining high rigidity, extends the π-electron conjugated system, causing its emission wavelength to redshift to the near-infrared region. This allows it to be extended to chemiluminescence detection and in vivo imaging in small animal models.

The invention discloses an in-vitro fixing auxiliary device for mouse embryo living body imaging. The in-vitro fixing auxiliary device comprises a support, a suction cup and a tray. An observation platform is formed on the bracket; the sucker is arranged on the observation platform, and an observation hole penetrating through the sucker in the axial direction is formed in the sucker; the tray is movably arranged under the suction cup in the vertical direction so as to be close to or away from the suction cup. Wherein a supporting part is formed on the tray, and a flexible opening is formed in the bottom of the supporting part, so that a mouse embryo can pass through the flexible opening; the bearing part is suitable for bearing a mouse embryo; the observation hole is partially constructed as a suction port which is suitable for sucking mouse embryos under negative pressure; when the tray is close to the suction cup, the suction opening is communicated with the bearing part. The fixation of the mouse embryo is realized by a method of combining liquid negative pressure suction and physical support, the stability is high, the activity of the mouse embryo can be maintained, and the method is suitable for long-term observation and experiment; meanwhile, the structure is simple, and the operation mode is flexible and efficient.

The invention relates to the technical field of biology, in particular to a construction method and application of a helicobacter pylori luciferase bacterial strain, the helicobacter pylori luciferase bacterial strain is obtained by introducing a Nanoluc gene into a helicobacter pylori genome, and the bacterial strain can efficiently express luciferase. The fluorescent probe can interact with a synthetic substrate furimazine to initiate lasting and bright bioluminescence, can be directly used for living body imaging, and is used for animal model construction and vaccine and therapeutic drug evaluation in the helicobacter pylori vaccine research and development process. Animal experiments prove that the helicobacter pylori luciferase strain interacts with furazine in the stomach of a living animal to emit bright and lasting bioluminescence, and is used for observing sustainable infection of the living animal. The method lays a foundation for further evaluation of helicobacter pylori vaccine protectiveness, and plays an important role in development and application of prevention and treatment vaccines and drugs.

The invention discloses an organic molecule with an ultrasonic activated luminescence property, a nanoparticle and application. The organic molecule comprises a porphyrin molecule, a cyanine molecule, a BODIPY molecule, an organic semiconductor polymer molecule containing a thiophene structure, an organic semiconductor polymer molecule containing a styrene structure and an anthracene derivative. According to the invention, through molecular structure screening, several kinds of organic molecules capable of emitting light through ultrasonic activation are used as illuminants, an amphiphilic polymer is used as a surfactant, and the nanoparticles capable of emitting light through ultrasonic activation are obtained through ultrasonic treatment. The invention also provides an application of the ultrasonic activated luminescence system in in-vivo imaging, the system takes an organic molecule with ultrasonic activated luminescence property as a luminescent substance, the ultrasonic activated luminescence system with near-infrared region emission can be constructed, and high-performance in-vivo imaging application is realized.

The invention discloses a head and neck cancer chemotherapy recurrence animal model and a construction method and application thereof, and relates to the technical field of animal model construction. Comprising the following steps: S1, culturing head and neck cancer tumor cells with fluorescence labels under an in-vitro condition; s2, subcutaneously planting the head and neck cancer tumor fluorescent cells in a groin part of a model animal; s3, 5 days after planting, imaging and observing subcutaneous tumor formation of the model animal, and performing periodic chemotherapy; s4, monitoring through a living body imaging system until a tumor fluorescence signal basically disappears, and determining tumor regression; s5, stopping chemotherapy, continuing feeding for 5-6 weeks, and observing the tumor recurrence condition of the model animal to obtain the head and neck cancer chemotherapy recurrence animal model. The method is easy and convenient to operate, short in experimental period and low in consumption, non-invasive dynamic monitoring can be achieved through the living body imaging technology, the variability of the result is small, the success rate is high, and an ideal platform is provided for head and neck cancer recurrence research and drug screening.

The application belongs to the technical field of biological medicine, and particularly relates to a pancreatic cancer specific binding peptide and a preparation method and application thereof. The pancreatic cancer specific binding peptide is a chelator-mediated radionuclide labeled HFL2c peptide; the radionuclide includes an imaging radionuclide or a therapeutic radionuclide; and the amino acid sequence of the HFL2c peptide is shown in SEQ ID NO. 1. Test results show that the polypeptide can be targeted to combine with the extracellular region of CLDN18.2, and the radionuclide labeled HFL2c peptide prepared by using the polypeptide can be used for nuclide imaging, realizes precise positioning, quantitative monitoring and high-sensitivity in-vivo imaging of CLDN18.2 expression, and is suitable for early screening, diagnosis and treatment monitoring of pancreatic cancer. Meanwhile, the therapeutic radionuclide labeled HFL2c peptide prepared by using the polypeptide can inhibit the growth of pancreatic cancer tumors.

The invention relates to a DNA tetrahedral nano-element as well as a preparation method and imaging application thereof, and belongs to the technical field of biological detection. The technical problems that in the prior art, the miRNA detection sensitivity is low, traditional tetrahedral DNA is difficult to enter cells, and the living body imaging specificity is insufficient are solved. According to the invention, a nano-element composed of a tetrahedral DNA frame, a polyguanine modified chain and a catalytic hairpin chain (H1 / H2) is constructed, and cycle signal amplification of to-be-detected miRNA is realized through a chain displacement reaction; after in-vitro verification, the probe is applied to macrophage targeted detection and ARDS mouse model lung imaging. According to the invention, efficient targeting of macrophages can be realized without a transfection reagent, excellent specificity is shown in vivo and in vitro, and a reliable tool is provided for early diagnosis and mechanism research of ARDS.