153 results about "Intravital Imaging" patented technology

The invention belongs to the technical field of nano biological materials, and particularly relates to an 800nm-near-infrared-excited 1525nm-shortwave-infrared-emission fluorescence nano material and a synthesis method thereof. The fluorescence nano material is a one-core / three-shell core-shell structure nanocrystal material composed of a nucleating center, a shortwave infrared light-emitting layer, an energy transmission layer and an energy absorption layer, wherein the nucleating center provides a crystal core for growth of the other three layers and limits the particle size of the nanocrystal; the shortwave infrared light-emitting layer is used for absorbing exciting light with specific wavelength and emitting shortwave infrared light; the energy transmission layer is used for transmitting energy between the energy absorption layer and shortwave infrared light-emitting layer; and the energy absorption layer is used for absorbing energy and transferring the energy to the energy transmission layer. The three-layer structure design widens the exciting light from 980nm to 800nm or so, and implements the Nd<3+>->Yb<3+>->Er<3+> energy transfer process. Due to the ideal excitation and emission wavelengths, the material can be used for deep tissue in-vivo imaging.

The invention belongs to the technical field of the preparation methods of the nanomaterials and specifically discloses a method for preparing water-soluble fluorescent silicon quantum dots by using a hydrothermal process. The method comprises the steps of firstly introducing an amino silane and a reducing agent into a solvent in which nitrogen is introduced and mixing, continuing introducing the nitrogen for protection for a while, next, transferring to a hydrothermal reaction kettle, and heating for reacting for a while, thereby obtaining the fluorescent silicon quantum dots having excellent chemical properties. The method has the advantages of low raw material cost due to the adoption of the amino silane as a silicon source, simple operation steps, direction synthesis in water phase, easy large-scale production, and being green and environment; the obtained quantum dots have high quantum yield and excellent chemical characteristics, and are small in particle size, evenly in distribution, non-toxic, good in biocompatibility and acid resistance; besides, the quantum dots have pH sensitive characteristic in a certain range, and can be widely applied to the biochemical and biomedical sensing fields such as biochemical detection, drug analysis, cell and living imaging and targeting tracing and also can be used as photovoltaic conversion and light-emitting display materials.

The invention discloses a preparation method and a time-resolved biological imaging application of thermally activated delayed fluorescent (TADF) organic material-based water-soluble long-life fluorescent nanoparticles. The nanostructure of the nanoparticles is represented by figure 1, a thermally activated delayed fluorescent molecule represented by CzPy is represented by a general formula (I). The method comprises the following steps: synthesizing the pure organic TADF micro-molecular material CzPy through adopting a one-step process, and rapidly injecting a CzPy / lecithin-polyethylene glycol mixed solution to deionized water to obtain the CzPy nanoparticles with good water solubility, high luminescence intensity and long fluorescence life. HELA cell dyeing marking and zebra blood vessel imaging results show that the nanoparticles have the advantages of long fluorescence life, low cytotoxicity and biotoxicity, stable spectrum signal, realization of time-resolved fluorescence imaging in cell or in vivo imaging, and good application prospect.

Devices and methods for optically scanning an in-vivo lumen, and for determining contact between an in-vivo device and an in-vivo lumen wall. The device may include an elongated housing having a cylindrical portion. At least one illuminating body may provide illumination at the circumference of the cylindrical portion, and at least one light sensor may sense light that penetrated and was scattered from the tissue of the in vivo lumen. The device may include means for preventing direct illumination from the illuminating body from reaching the light sensor and for decreasing the amount of direct light reflection from the outer surface of the in vivo lumen onto the light sensor.

The invention discloses a method for preparing a large number of carbon quantum dot biology imaging agents and carbon quantum dot biology imaging agents. The method provided by the invention comprises the followings: glycerol with the mass ratio of 1 to 99 percent and a silane coupling agent with the mass ratio of 99 percent to 1 percent are mixed for serving as a presoma, and under inert gas protection, full reaction is performed at the temperature of 160 DEG C to 270 DEG C so as to obtain the carbon quantum dot biology imaging agent. The method provided by the invention has the advantages that the yield of the carbon quantum dot biology imaging agent prepared is high, and under different excitation wavelengths, different colors of fluorescent light can be stimulated; during the preparing process, processes of product surface passivation, surface decoration and the like can be accomplished in one step; a thin-film device can be prepared; the carbon quantum dot biology imaging agent is low in cytotoxicity, can be dissolved in water or an organic solvent easily, and can be used in the fields of cell imaging, or living imaging and the like; the imaging agent has the advantages of photobleaching resistance and the like.

The invention provides a targeting material based on an indolium squarine dye, a preparation method thereof, a fluorescent nano-particle, and a preparation method of the fluorescent nano-particle. Bromine, hydrogen or methyl-substituted indolium squarine is coupled with targeting polypeptide RGD molecules or is respectively coupled with RDG molecules and an anticancer drug camptothecin to obtain amphiphilic diagnosis and treatment molecules. The molecules can be self-assembled in water to form the stable nano-particle with a uniform size. The nano-particle prepared in the invention can emit near infrared fluorescence, and can reduce the interference of background fluorescence and increase the signal penetrating depth in an in-vivo imaging process; the nano-particle has a tumor targeting performance, can be efficiently enriched in a tumor tissue are and can realize accurate in-vivo tumor fluorescence imaging; and the nano-particle has photodynamic properties, and can achieve effective photodynamic therapy or photodynamic therapy-chemotherapy combined therapy for tumors. So the nano-particle prepared in the invention has a good application prospect in the field of accurate diagnosisand treatment of cancers.

The invention relates to a lentivirus vector expression system of luciferase and an application thereof. Particularly speaking, in the invention a luciferase lentivirus expression system with FG12-Luc2 as core plasmid is successfully constructed, and a PVTT-1 cell line from tumor thrombus tissue of liver cancer patients is established by the system. Through the lentivirus expression system, liver cancer tissue is successfully marked; and by experimental methods such as subcutaneous transplantation and orthotopic liver cancer transplantation, a mouse orthotopic transplantation liver cancer metastasis model is established which is suitable for serial passage, is applicable to in-vivo imaging, and can realize real-time monitoring of the growth and metastasis conditions of tumors in the body.

The invention discloses a cyanine fluorescent dye having the structural formula represented by one of the formula I and the formula II defined in the specification. The cyanine fluorescent dye provided by the invention has both the excitation wavelength and the fluorescence emission wavelength reaching a near infrared region, and the Stokes shifts are each greater than 60 nm; the background fluorescence of the biological environment and the self-quenching and self-absorption effects of the dye in the application of living imaging are significantly reduced, the signal-to-noise ratio is improved, and the sensitivity is improved. The synthesis method of the fluorescent dye provided by the invention is simple, the raw materials are easy to obtain, and the yield is high; the fluorescent dye provided by the invention has good effect in cell imaging and living imaging of small animals, and has high light stability.

The invention discloses a fluorescent compound which has a structure as shown in a general formula (I). The invention also discloses a preparation method of the compound and application of the compound in preparation of a diagnostic agent or an imaging agent for tumor detection and / or biological imaging such as in-vivo imaging. The fluorescent compound disclosed by the invention can emit fluorescence as high as 1600nm in an NIR-IIb region, wherein QY is as high as 9-18%. The compound has excellent imaging capability in superficial blood vessels and visceral organs of mice, has high resolution, and enhances the signal-to-noise ratio of an NIR-IIb region.

The invention discloses a preparation method for a fluorescent star-shaped block copolymer and application of the fluorescent star-shaped block copolymer serving as a medicine carrier. According to the preparation method, the fluorescent star-shaped block copolymer is prepared according to ring opening polymerization by taking amphipathic polylactic acid-polyphosphoester as an arm and taking a perylene bisimide derivative as a core. The fluorescent star-shaped block copolymer is self-assembled in an aqueous solution to form fluorescent supramolecular micelles, have good fluorescence characteristics, and can serve as a fluorescent probe and be used for cell and in-vivo imaging. The fluorescent supramolecular micelles prepared by the preparation method can effectively carry hydrophobic medicines and realize pH stimulation release. The analysis of a laser scanning confocal microscope and a flow cytometer show that the fluorescent supramolecular micelles are easily swallowed by cancer cells and can realize cellular internalization. In in-vitro and in-vivo tumor growth inhibition experiments, compared with a free medicine, the inhibition effect of the fluorescent supramolecular micelles to tumor growth is more obvious, so that the fluorescent supramolecular micelles have potential application value in the fields of biological imaging and cancer treatment.

The invention provides large stokes displacement fluorescent protein CyOFP and application of the fluorescent protein to microimaging and highly-sensitive living creature light-emitting imaging. The fluorescent protein is obtained through mNeptune site directed mutagenesis, the 1-230 positions of the amino acid sequence of the fluorescent protein sequentially correspond to 4-234 positions shown by the SEQ ID No: 2, or the fluorescent protein can be further obtained by conducting gene synthesizing on the fluorescent protein DNA segment and expression. The invention further provides a novel BRET system and fusion protein Antares obtained through system optimization. The CyOFP can be stimulated by blue light, and a quite high quantum yield is achived; the CyOFP and the enhanced green fluorescent protein (EGFP) are jointly stimulated by monochromatic light at the same time in a two-photon mode, and the resolution ratio of two-photon imaging is raised; a novel BRET system formed together with fluorescent protease NanoLuc greatly improves the depth and sensitivity of living creature imaging.

The invention discloses a near-infrared fluorescent molecular probe for detecting hydrogen sulfide and a preparation method and application of the near-infrared fluorescent molecular probe. The near-infrared fluorescent molecular probe has a molecular formula of C31H29N4O+, and has a structure formula shown in the description. Compared with the prior art, the near-infrared fluorescent molecular probe disclosed by the invention is good in selectivity and good in response effect on hydrogen sulfide, and in addition, can be well applied to living imaging and the like because of the properties ofnear-infrared absorption and fluorescence emission. In addition, the near-infrared fluorescent molecular probe is simple in synthesis process, low in cost and high in yield.

The invention provides a near-infrared fluorescent heptamethine cyanine dye and application thereof in preparation of a tumor diagnosis agent and / or an imaging agent. The near-infrared fluorescent heptamethine cyanine dye is a compound DZ-1, wherein the side chain of the compound DZ-1 is (CH2)4SO3H, so that the compound DZ-1 can be directly dissolved in water; in addition, the near-infrared fluorescent heptamethine cyanine dye can be applied by intravenous injection during living imaging, thus achieving the same treatment and applying mode as clinic ICG (Indocyanine Green); the compound DZ-1 can be specifically absorbed by hepatoma carcinoma cell and is gathered at the tumor part to support no-intrusive NIRF imaging, so that the tumor growing part can be detected; the fluorescence intensity at the tumor part, the specificity and the targeting performance are prior to those of clinically-used ICG. Therefore, the tumor diagnosis agent and / or the imaging agent adopting the compound DZ-1 are high in tumor targeting performance and high in stability.

The invention relates to a novel human pancreatic cancer nude mouse model construction method for living imaging research and a use thereof. A human pancreatic cancer PANC-1 cell is stably transfected with a plasmid carrying a luc gene through a Lonza nuclear transfection system, a cell strain PANC-1-LUC for stable luciferase expression is screened through puromycin and PANC-1-LUC nude mouse transplant subcutaneous sarcoma is further constructed. An in vitro and vivo bioluminescence detection result proves that the PANC-1-LUC cell strain can stably express luciferase for a long time. Compared with a lentiviral vector-mediated bioluminescence pancreatic cancer nude mouse model, the built pancreatic cancer nude mouse model has a short tumor formation incubation period and a high tumor formation rate, satisfies PANC-1 transplantation tumor growth features and is suitable for living imaging research.

A fixing and observing device for living imaging of animal spinal cords comprises a bottom plate (1), a left movement adjusting device (2), a right movement adjusting device (3) and an embedded observation window (4). The left movement adjusting device (2) and the right movement adjusting device (3) are fixedly connected to the bottom plate (1) through screws. The embedded observation window (4) is in insertion connection with rectangular protrusions of horizontal positioning sliders on the left movement adjusting device (2) and the right movement adjusting device (3) through rectangular holes in V-shaped frames to be positioned. By the fixing and observing device, operations for installing the embedded observation window can be facilitated to improve stability of the spinal cords, and dynamic observation of post-operation two-photon spinal-cord imaging can be also facilitated. The horizontal positioning sliders on the left movement adjusting device and the right movement adjusting device can guarantee horizontal stable internal fixation states for implant operations and can further provide stable external fixation support during post-operation observation.

The invention provides a fluorescent probe for distinguishing and detecting mercaptan and monitoring Cys / GSH metabolism as well as a preparation method and application of the fluorescent probe. The Chinese name of fluorescent probe is 10-(diethylamino)-3-((7-(diethylamino)-3-formyl-2-oxo-2H-chromen-4-yl) oxy)-5, 6-dihydrobenzo [c] xanthene-12-onium perchlorate, the English name is 10-(diethylamino)-3-((7-(diethylamino)-3-formyl-2-oxo-2H-chromen-4-yl)oxy)-5,6-dihydrobenzo[c]xanthen-12- ium perchlorate,, which is named as CM-O-AC. The invention provides a red fluorescent probe with multiple binding sites, good water solubility and large Stokes shift. The probe displays three different emission channels for distinguishing and detecting Cys / Hcy and GSH, and is successfully applied to HeLa celland living body imaging. In addition, the probe can realize recognition of the Cys / GSH metabolite SO2 at a cellular level, and realizes reversibility of a fluorescence signal under the action of H2O2. The probe can be used as a visual biological metabolism tool to be applied to physiological and pathological processes. The detection process is simple, sensitive and rapid, and the detection resultis accurate.

The invention discloses a high-stability second near-infrared small-molecule fluorescence probe and a production method and application thereof. The novel high-stability second near-infrared small-molecule fluorescence probe is designed and synthesized, has strong tissue penetration capability, is almost free from interference of autofluorescence, and can achieve living second near-infrared fluorescent and photoacoustic imaging with high temporal-spatial resolution. The target probe can conduct radioactive 125I isotope labeling by means of a chloramine T method to achieve SPECT / CT imaging of small living animals. Meanwhile, the target probe has good active targeting to tumor tissues, a photothermal therapy effect on tumors is significant, and diagnosis and treatment integration of the tumors is achieved.

The invention obtains a Gaussia Luciferase (GLuc) active fragment by deleting 37 amino acids at the N-terminal of the GLuc, which is named as GLuc 148. Being the same as the wild type Gluc, the GLuc 148 has high luminescence activity and can achieve stable biochemical properties without the presence of complementary factors, but the GLuc 148 has a smaller molecular weight and performs non-secretory type expression in eukaryocytes. Thus, the GLuc 148 is more suitable for being used as a report factor and a fluorescent energy donor, and can be used in the technical fields of cell and in-vivo imaging, high-flux screening, microorganism detecting and the like.

The invention discloses a synchrotron radiation X-ray microscopic CT suitable for imaging of living small animal limbs and a fixing device. The fixing device comprises an objective table for an anesthetized small animal to be placed, a column fixed to the objective table and a lead cover which covers the objective table and seals the upper part and periphery of the objective table, a synchrotron radiation light source comprises the fixing device, the objective table of the fixing device is fixed to a rotating sample table of the synchrotron radiation X-ray microscopic CT, and moreover, the axis of a sleeve on the objective table is coaxially arranged with the rotating axis of the rotating sample table. The fixing device of the synchrotron radiation X-ray microscopic CT can be used for integrally and locally fixing the living small animal, and meanwhile, the influence of high-dose radiation on the trunk part outside the joints of the limbs can be avoided; the survival of the living small animal can be protected to the greatest extent, and long-term animal experiments can be conveniently carried out.

A method of and device for acquiring in-vivo images or quantitative / qualitative data (perfusion, blood flow, vascularization, contrast enhancement, selective blood supply management) of interior parts of the human body (20) is described, using an imaging system (21) and including the steps of positioning the body (20) relatively to the imaging system (21), applying a respiratory resistance device (10) to the respiratory system of the body (20), and performing an image acquisition step during or concomitantly an inhalation / inspiration / suction or exhalation / Valsalva / expiration phase, during which the body provides suction or exhalation against a resistance as provided by the respiratory resistance device (10).

The invention relates to the field of biological medicines, and relates to an N,N-diphenylamino-modified beta-carboline indolium salts, a preparation method and application of the N,N-diphenylamino-modified beta-carboline indolium salt. The beta-carboline indole onium salt has a structure as shown in a general formula I, and can be activated in an acidic microenvironment of tumor tissues by utilizing an ICT principle to selectively and quickly generate pH-sensitive near-infrared fluorescence at a tumor part. According to a specific implementation method, a solution of the beta-carboline indole onium salt is sprayed or locally injected to a tumor focus part and surrounding tissues, and rapid and selective fluorescence imaging and tracing are conducted on the tumor focus tissues by using a fluorescence endoscope or a living imaging instrument. The beta-carboline indole onium salt has relatively high tumor tissue fluorescence imaging selectivity and relatively low background fluorescence interference, and can be used for accurately detecting tumors.

The present invention provides an in vivo imaging method that facilitates the diagnosis of Parkinson's disease (PD) at an early stage. Early diagnosis is particularly advantageous as neuroprotective treatment can be applied to healthy neural cells to delay or even prevent the onset of debilitating clinical symptoms.

The invention relates to mouse typhus salmonella UF110lux and application of mouse typhus salmonella UF110lux in living body imaging. The strain is conserved in the China General Microbiological Culture Collection Centre specified by the State Intellectual Property Office on March 13, 2012, and the conservation number is CGMCCNo.5893. The mouse typhus salmonella UF110 is a strain obtained by the fact that a spv gene on a virulence plasmid is knockout, and can be used for researching the function of mouse typhus salmonella virulence gene spv as a control. The UF110lux is a strain which is constructed on the basis of the mouse typhus salmonella UF110 and has the stable biological luminescence property. According to the invention, the shortage of a traditional fluorescent mark is overcome, and the invasion, proliferation and diffusion of the mouse typhus salmonella in a living body of mice can be monitored dynamically in real time by using a living body imaging instrument. The invention provides a convenient tool for further study on a pathogenic mechanism of mouse typhus salmonella.

The invention provides a near-infrared A beta fluorescence imaging agent with high signal-noise ratio, as well as a preparation method and application thereof. An aggregation-induced emission dye hasa structure as shown in the formula I. In addition, the invention also provides application of the A beta fluorescence imaging agent in brain tissue slice fluorescent marking and living imaging. The tests show that the aggregation-induced emission dye provided by the invention has the advantages of near-infrared emission, high light stability, good biocompatibility, high signal-noise ratio and thelike, and can be used for accurately positioning an A beta plaque in an APP / PS1 transgenic mice brain tissue slice, so that the in-situ information such as the number of A beta plaques and real-timeform is acquired in a high fidelity way. Particularly, the imaging agent has favorable blood-brain barrier penetrability, and can be applied to in-vivo real-time imaging of the APP / PS1 transgenic micebrain A beta plaque. The formula I is shown in the description.

The invention discloses the preparation of Magnetic Resonance & Near-infrared Fluorescence Targeted Nanoprobes (MR & NIR-NPs) and its application in in vivo imaging and photothermal therapy. The skeleton material is selected, modified to reduce protein adhesion, grafted with nuclear magnetic imaging functional groups and tumor targeting groups, and then electrostatically self-assembled with near-infrared fluorescent dyes to form nanoprobes. group. The nuclear magnetic & near-infrared fluorescent targeting nanoprobe can target tumor tissue, has excellent MR and NIR multi-modal targeting imaging capabilities, and realizes photothermal therapy.

The invention relates to a near-infrared long-afterglow luminescent material, a fluorescent probe as well as a preparation method and application thereof. The chemical composition of the near-infraredlong-afterglow luminescent material is LaGa1-x-yCrxSbyO3, x is greater than or equal to 0.001 and less than or equal to 0.03, and y is greater than or equal to 0.001 and less than or equal to 0.03. According to the near-infrared long-afterglow luminescent material provided by the invention, LaGaO3 is used as a matrix, and ions Cr < 3 + > and Sb < 3 + > are doped to optimize the afterglow performance; the obtained near-infrared long-afterglow luminescent material can be effectively excited by X-rays to generate afterglow emission at 750nm, the afterglow lasting time is as long as 500 hours, and the near-infrared long-afterglow luminescent material has a wide application prospect in photoelectric devices or biological imaging; particularly, the nano-granular near-infrared long-afterglow luminescent material can be prepared into a fluorescent probe for in-vivo imaging, and a better imaging effect can be obtained under the excitation of low-dose X-rays.

The invention discloses a recombinant avian influenza virus carrying NanoLuc gene and application thereof in a living imaging mouse model. The invention provides a DNA fragment of which the nucleotidesequence is as shown in SEQ ID NO.1, and the DNA fragment can be used to construct a recombinant plasmid, a recombinant virus and a mouse model. In the invention, a recombinant avian influenza viruswhich is similar to the wild type virus in the replication ability and pathogenicity is constructed. In the SPF chick embryo passage, the NanoLuc luciferase gene with good stability can be detected inthe collected 4 generations of virus solutions. In the invention, a living imaging mouse model which has a visualization function is successfully constructed, and the distribution position of the influenza virus in a mouse can be detected without dissecting the mouse. Longitudinal assessment can be performed to observe the dynamic process of influenza virus infection in the same mouse for a continuous period of time, and a visualization tool is supplied for the study of influenza virus.

The invention discloses a mouse model capable of monitoring NF (nuclear factor)-kB activity in the liver by virtue of in-vivo imaging and a construction method of the mouse model. The construction method comprises the following steps: constructing a recombinant vector which carries a phage integrase recognition site attB gene, an NFkB gene and an Fluc (firefly luciferase) reporter gene, and introducing the recombinant vector into the mouse body by virtue of a hydrodynamic method to obtain the mouse model, wherein the Fluc reporter gene regulated and expressed by the NFkB gene is integrated in the liver of a mouse. The mouse model disclosed by the invention can be used for evaluating the adjustment effects of different factors on the NF-kB activity in vivo, also can be used as an evaluation model of interferon or inflammatory factor regulating medicaments, molecules and viruses in natural immunity, and can be widely applied to various categories of biomedical animal experiments.

The invention discloses a near-infrared two-zone dye FT-3T, the absorption peak of the FT-3T molecule part and the whole emission peak are in the near-infrared two-zone range by adjusting an intermediate coupling unit, a preparation method of the dye, and nanoparticles with good water solubility prepared by a nano coprecipitation method are provided, and the near-infrared two-zone dye FT-3T can be used for preparing the near-infrared two-zone dye FT-3T. The nano-particles have strong two-region fluorescence emission and good photo-thermal performance, can be applied to preparation of a contrast agent for in-vivo imaging excited by 1064nm laser, can also be applied to preparation of a two-region fluorescence guided photo-thermal diagnosis and treatment reagent, and has a wide application prospect.

The invention belongs to the technical field of nano biological materials, and particularly relates to a wavelength-adjustable near-infrared second window long afterglow nano probe and a synthesis method thereof. The long-afterglow nanoprobe is a nanocrystal with a core-shell structure, a long-afterglow luminous core layer is arranged inside the nanocrystal, and an inert shell layer is arranged outside the nanocrystal; the core layer is used for absorbing energy and emitting long afterglow signals; the inert shell layer is used for reducing the quenching effect of an external quenching group on a long afterglow signal. According to the invention, the long afterglow emission wavelength is adjusted by changing the doping type of rare earth ions; by changing the doping amount of rare earth ions, the size of a core layer and the thickness of a shell layer, the signal intensity and attenuation duration of long afterglow luminescence are adjusted. The long afterglow probe does not need to be excited in real time by an external light source, biological background noise caused by the external light source is avoided, and the resolution and the signal-to-noise ratio of biological imaging can be improved. The long afterglow probe has wide application prospects in the aspects of information coding and storage, multi-channel biological detection, living body imaging and analysis, surgical navigation and the like.