15 results about "Intravital Imaging" patented technology

1. Name of the product in this design: Live Imaging Organ Fixation Instrument. 2. Purpose of this design: To fix organs or tissues of live experimental animals during microscopic imaging. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

This utility model discloses a large-spot laser application device, including a device box, and an angle adjustment plate, a laser light source, and a projection plate disposed inside the device box. The two angle adjustment plates are respectively disposed on both sides above the projection plate, and a laser light source is installed on each of the two angle adjustment plates. The irradiation direction of the laser light source is aligned with the projection plate, and the laser light source and the angle adjustment plate are connected to form an adjustable irradiation angle structure. This utility model uses a laser light source to replace the traditional LED light source, halogen light source, and xenon lamp light source, which not only extends the service life of the light source, but also makes the light source smaller and more conducive to the integration of the entire structure. It can be directly applied to the excitation of light sources in CCD camera multicolor fluorescence chemiluminescence gel imaging systems and animal and plant live imaging systems without replacing the light source.

This invention relates to a medaka hatching enzyme, its preparation method, and its application. The preparation method of the medaka hatching enzyme includes the following steps: 1) collecting fertilized medaka eggs; 2) incubating the fertilized medaka eggs with embryo culture medium in a 26℃ incubator for 6 days, and collecting mature embryos; 3) adding NaCl solution and Tris-HCl buffer (pH=8.0) to the mature embryos, grinding them, collecting the filtrate, and allowing it to stand overnight at 4℃; 4) centrifuging the filtrate at 15000 rpm for 15 minutes, and extracting the supernatant to obtain the medaka hatching enzyme solution. The preparation method is simple to operate, and the obtained medaka hatching enzyme has high activity, which can help to quickly remove the medaka eggshell artificially. The naked medaka embryos obtained by this method can be directly used for in vivo imaging and in-situ quantitative analysis, and embryo model construction, providing support for subsequent genetic, developmental biological, and toxicological studies of medaka embryos.

This application provides a ratiometric fluorescent probe for detecting divalent copper ions, its preparation method, and its application. The ratiometric fluorescent probe comprises fluorescently labeled human serum albumin with isothiocyanate and a dye compound; the fluorescently labeled human serum albumin with isothiocyanate is coated on the surface of the dye compound; the dye compound is a compound with the chemical formula shown in Formula I, II, III, or IV. The ratiometric fluorescent probe provided in this application can be applied to various Cu... 2+ The detection scenario, and can detect Cu 2+ Quantitative detection, on-site rapid testing, and live imaging analysis are performed.

Providing imaging equipment 2 capable of obtaining high-precision images within living organisms. [Solution] The imaging device 2 has a subshaft 6, a first cable 8a, a second cable 8b, and a head 10. The head 10 has a cylinder 12, a filler 14, a cantilever 31, a guide 18, a camshaft 20, and a cam 22. The cantilever 31 includes a first sensor 30a and a second sensor 30b. The guide 18 has a guide groove 32. The cantilever 31 is inserted into this guide groove 32. The rotation of the subshaft 6 causes the cam 22 to rotate. The rotation of the cam 22 causes the cantilever 31 to reciprocate while being guided by the guide groove 32.

The utility model relates to observation device technical field belongs to a kind of mouse live imaging fixing device based on inverted microscope, including fixed on the fixed device box base body and base of microscope platform, the fixed device box base body bottom is fixedly connected on base, the upper portion of fixed device box base body is equipped with detachable seal cover, the base is opened on the observation window, the fixed device box base body perimeter is also opened experimental fixed hole. The utility model can flexibly match the tissue of surgical exposure mouse and the mouse of surgical installation observation window are observed using inverted microscope, reduce the development cost of multiple sets of fixed device, can also achieve certain mouse physiological state maintenance function, and compatible various equipment required by experiment, simplify live imaging observation procedure.

This invention relates to a class of dihydroxanthine chemiluminescent groups, their preparation methods, and applications. The dihydroxanthine chemiluminescent group developed in this invention, compared to the traditional phenolic dioxane chemiluminescent group which uses two rigid six-membered rings to fix the double bond conformation, exhibits a higher luminescence quantum yield and a longer emission wavelength. More importantly, it possesses a reactive aldehyde group, which, while maintaining high rigidity, extends the π-electron conjugated system, causing its emission wavelength to redshift to the near-infrared region. This allows it to be extended to chemiluminescence detection and in vivo imaging in small animal models.

This invention belongs to the fields of immunology and molecular biology, specifically relating to a nanobody targeting the sialylated glycan antigen (KL-6) and its applications. The nanobody comprises any one of five sets of complementary determinant regions. This invention also provides a nucleic acid molecule encoding the nanobody, a carrier and host cell containing the nucleic acid molecule, a fusion protein and conjugate containing the nanobody, and a pharmaceutical composition containing the above substances. The nanobody of this invention exhibits high specificity and affinity, and can be used to prepare KL-6 detection reagents, in vivo imaging probes, or therapeutic products, particularly showing broad application prospects in the diagnosis and treatment of KL-6-related diseases such as interstitial lung disease.

This invention discloses a near-ultraviolet emitting copper nanocluster, its preparation method, and its applications. The chemical composition of the copper nanocluster is Cu4MPD7, and its fluorescence emission peak is located near 343 nm. The preparation method employs a simple one-pot aqueous phase method, using m-phenylenediamine as a reducing agent and ligand, reacting it with copper sulfate at room temperature followed by centrifugation. This nanocluster can be used as a fluorescent probe, emitting near-ultraviolet emission through its surface Cu... 2+ The specific binding with glyphosate induces fluorescence enhancement, enabling highly sensitive and selective detection of glyphosate, which has been successfully applied to the analysis of actual samples. Furthermore, due to its near-ultraviolet emission properties and excellent biocompatibility, this nanocluster also has significant application potential in fields such as biolabeling, in vivo imaging, disease treatment, catalytic materials, and the preparation of composite nanomaterials. This invention features a simple process, low cost, and environmental friendliness, providing new materials and methods for pesticide residue detection and the development of functional nanomaterials.

An imaging device (2) has a sub-shaft (6), a first cable (8a), a second cable (8b), and a head (10). The head (10) has a cylinder (12), a filler (14), a cantilever (31), a guide (18), a camshaft (20), and a cam (22). The cantilever (31) includes a first sensor (30a) and a second sensor (30b). The guide (18) has a guide groove (32). The cantilever (31) is inserted into the guide groove (32). The cam (22) is rotated by the rotation of the sub-shaft (6). Due to the rotation of the cam (22), the cantilever (31) reciprocates while being guided by the guide groove (32).

The application discloses a vaccine composition containing a recombinant G protein of Hendra virus. Specifically, a multimer and a monomer are constructed by using the recombinant G protein of Hendra virus, and a better in-vivo protection effect than DNA is obtained; and the construction of the tandem protein also effectively solves the problem of poor broad-spectrum property of the vaccine. Meanwhile, the in-vivo protection effect evaluation of the vaccine can be quickly and safely realized by detecting the fluorescence residue in the mouse in-vivo through a VSV pseudovirus live imaging model.

The present application relates to a TAL H180 The application relates to application of a gene mutant in increasing biosynthesis amount of 3-aryl acrylic acid or increasing light emission intensity of an organism or a cell. The application provides an innovative solution for creating an efficient self-luminous plant, and can be widely applied to the fields of biosensor development, in-vivo imaging, ornamental plant cultivation and the like, and has important scientific value and broad application prospect.

Provided are a near-infrared immunofluorescent conjugate and use thereof. It has been found that a near-infrared fluorescent dye can be conjugated with an antibody to form a conjugate, which can be loaded into a modified chitosan carrier, thereby preparing a near-infrared immunofluorescent probe with an imaging function that can be administered orally. The conjugate of the near-infrared fluorescent dye and the antibody has emission wavelength coverage in a range of 700-1700 nm in a near-infrared region, and has relatively low light scattering, relatively high in-vivo imaging resolution, and relatively deep penetration depth. In addition, the conjugate of the near-infrared fluorescent dye and the antibody has the advantages of low cost, biological safety, suitability for drug formation, and the like. The conjugate of the modified chitosan-loaded near-infrared fluorescent dye and the antibody based on formula I can be administered by means of oral delivery, intravenous injection, and the like, and has good compliance.

This invention discloses a glioma cell line suitable for in vitro and in vivo monitoring of glioma growth, its construction method, and its applications. Based on GL261-luc, GL261-EGFP-luc is constructed to achieve in vitro and in vivo monitoring of glioma growth. By constructing an orthotopic glioma transplantation animal model using GL261-EGFP-luc, in vivo imaging techniques based on the interaction between luciferase and luciferin can be used to study the dynamic development and changes of glioma cells in the mouse brain, and in vitro, the distribution of glioma cells in tumor tissue sections can be studied using EGFP. The GL261-EGFP-luc constructed in this invention not only retains the ability of GL261-luc cells to monitor glioma growth in vivo, but also allows for precise cellular-level examination of tumor tissue sections in vitro, clearly distinguishing glioma boundaries. This provides a more precise and efficient glioma cell line for glioma research, enabling precise localization of each cancer cell and providing an efficient tool for precision treatment of gliomas.

This invention belongs to the field of pharmaceutical technology and relates to a novel medical use of caffeine for premature infants, specifically its application in improving jaundice in premature infants. This invention analyzes clinical data from premature infants, constructs a mouse model of jaundice, and conducts in vivo imaging and gene knockout verification experiments in premature mice. It was found that clinical caffeine treatment can effectively reduce serum bilirubin levels in premature infants; in jaundice mice and premature mouse models, caffeine intervention significantly reduces brain tissue and serum bilirubin concentrations; gene knockout mouse experiments confirmed that caffeine enhances hepatic bilirubin uptake by upregulating OATP1A / 1B. The caffeine in this invention shows a significant effect in improving neonatal jaundice. Furthermore, caffeine is a commonly used medication for premature infants, is easy to use, and is readily accepted by medical staff and families, making it suitable for relieving apnea combined with jaundice symptoms in premature infants.