105 results about "Intravital Imaging" patented technology

The invention discloses an application of pegylated irinotecan in preparation of a medicine for preventing and / or treating triple-negative breast cancer brain metastasis diseases, which is shown by in-vivo imaging observation and lifetime observation of high, medium and low dose groups of pegylated irinotecan with a specific structure. The high-dose group, the medium-dose group and the low-dose group all have a definite anti-tumor effect, bioluminescence signal values are inhibited to different degrees, the lifetime is prolonged, and the lifetime of the high-dose group and the lifetime of the medium-dose group are remarkably longer than that of the NKTR-102 group.

The application provides a chemical luminescence substrate with high chemical luminescence intensity, long wavelength and good stability, and a preparation method and application thereof. The chemical luminescence substrate has a structure shown in formula I. In addition, the application provides spontaneous fluorescence wavelength and intensity of the chemical luminescence probe in a physiological environment, and application of the chemical luminescence probe in in-vivo imaging. Test results show that the chemical luminescence probe provided in the application has long-wavelength spontaneous fluorescence (600 nm), can effectively penetrate skin tissue, has high spontaneous fluorescence intensity (> 10 7 p / s / cm 2 / sr), excellent thermal stability (without 1,2-dioxetane structure) and diversified detection groups (suitable for different detection models).

The invention belongs to the technical field of nano biological materials, and particularly relates to a near-infrared two-region excitation and emission lanthanide nano fluorescent probe as well as a preparation method and application thereof. Lanthanide ions with near-infrared two-region absorption characteristics are used as sensitizers, nanocrystalline with a co-doped structure formed by the lanthanide ions and activator ions is used as a core, and a pure-phase matrix material shell layer which is the same as a core lattice is grown on the surface of a core particle in situ. The nano fluorescent probe can generate tunable near-infrared two-region fluorescence emission with the wavelength of 1000 to 3000 nm under the excitation of near-infrared light with the wavelength of more than 1000 nm. The near-infrared two-region excitation and emission characteristics of the nano fluorescent probe can provide higher tissue penetration depth and signal-to-noise ratio for in-vivo imaging. The nano fluorescent probe shows excellent performance in living body angiography, dynamic observation of intestinal tracts and multi-channel near-infrared two-region imaging, and has wide application prospects in the fields of biomedical detection, early diagnosis of diseases, surgical navigation and the like.

1. The name of the design product: In Vivo Imaging System. 2. The use of the design product: This design product is used for chemiluminescence fluorescence imaging, small animal in vivo imaging or plant in vivo imaging. 3. The design key points of the design product: lies in the shape. 4. The picture or photo that best shows the design key points: perspective view.

The invention belongs to the technical field of GPCR drug research and development, and discloses a ratio type cAMP fluorescent probe RaCamp and a construction method of a transgenic mouse, the probe RaCamp is formed by series fusion of G-Flamp2 fluorescent protein and mCherry fluorescent protein through a flexible linker, and the preparation method of the probe RaCamp comprises the following steps: S1, obtaining an mCherry fragment; s2, a G-Flamp2 linear carrier is obtained; s3, homologous recombination; s4, converting and screening; the construction method of the transgenic mouse comprises the following steps: T1, designing a carrier; t2, microinjection of fertilized eggs; t3, embryo transplantation and reproduction; the invention has the beneficial effects that mCherry is added as an internal reference protein on the premise of keeping the sensitivity of the original G-Flamp2 probe, and the selected connecting peptide (GGGGS) 2 enables the expression quantity of the two proteins in cells to be accurately controlled to be 1: 1, so that the problem of background interference in cell observation of in-vivo imaging is well solved. And the sequence is inserted into a Rosa26-LSL box, so that the construction of a Rosa26-LSL-RaCamp mouse is realized.

The invention relates to the technical field of biology, in particular to a cryopreserved stem cell diluent and application thereof.The cryopreserved stem cell diluent further comprises cysteine and polyvinylpyrrolidone besides a compound electrolyte injection, the concentration of cysteine in the cryopreserved stem cell diluent is 1-10 mM, and the concentration of polyvinylpyrrolidone in the cryopreserved stem cell diluent is 1-10 mM. The concentration of the polyvinylpyrrolidone in the cryopreserved stem cell diluent is 0.02 to 0.1 g / mL. The cryopreserved stem cell diluent can improve the cell activity and cell distribution after the cryopreserved cell preparation is recovered, and can monitor the application of cells in rat living body imaging.

The invention relates to lithospermum erythrorhizon rhizome exosome vesicles as well as a preparation method and application thereof in preparation of antitumor drugs. The preparation method comprises the following steps: juicing lithospermum erythrorhizon, filtering, and collecting filtrate; and centrifuging the filtrate, collecting the precipitate, re-suspending, and filtering to obtain a solution containing the lithospermum erythrorhizon exosome vesicles. Compared with the prior art, the lithospermum erythrorhizon rhizome exosome vesicles extracted by the method can inhibit malignant progression, including proliferation and migration, of lung cancer, and cell and animal living imaging proves that the lithospermum erythrorhizon rhizome exosome vesicles can be taken by cells and can be enriched in tumor tissues at the same time.

The application belongs to the technical field of fluorescent probe, and particularly relates to an aminopeptidase fluorescent probe and a preparation method and application thereof. In order to develop an APN near-infrared fluorescent probe suitable for in vivo imaging, a center-position ester group substituted pentamethine cyanine dye platform (Cy5-COOM) is used, and based on an "ester group to carboxyl group" conversion strategy, an aminopeptidase (APN) fluorescent probe Cy5-APN is developed. The probe is firstly hydrolyzed by APN, then a 1,6-elimination reaction occurs, and finally a strong fluorescent center-position carboxyl group substituted pentamethine cyanine dye Cy5-COO is released. In addition, in view of the characteristics that cancer cell surfaces overexpress APN, the probe can distinguish cancer cells / tissues and normal cells / tissues with high contrast, and therefore has great application potential in fluorescence-guided tumor surgery.

The application provides a method for improving the precision of blood vessel information extraction in OCTA live imaging, comprising the following steps: performing two-dimensional wavelet decomposition on an image to obtain a low-pass frequency component, a horizontal structure detail component, a vertical structure detail component and a diagonal structure detail component; selecting the vertical structure detail component to perform fast Fourier transform to obtain a frequency spectrum diagram of the vertical structure detail component; performing frequency domain filtering on the frequency spectrum diagram of the vertical structure detail component by using a band-pass filter to obtain a filtered frequency domain signal; performing inverse fast Fourier transform on the filtered frequency domain signal and wavelet inverse transform to reconstruct a filtered image; comparing each pixel point in the reconstructed filtered image and the original image and taking the minimum value; iteratively performing the above process multiple times to reconstruct a deep denoising image; and the application can effectively remove the stripe motion artifacts existing in the OCTA live imaging result, and has very good reservation capability for image quality and blood vessel information.

The utility model relates to the technical field of plant living body imaging, and discloses a plant living body imaging and simulation illumination device which comprises a box body, when the device is used, an LED plant growth lamp can be turned on firstly through an arranged environment simulation assembly, and light irradiates a plant through a through hole; light is dispersed around plants through the astigmatic lenses arranged on the through holes, so that more real natural light is simulated, meanwhile, natural light such as sunny days, cloudy days and night with moonlight can be simulated through the colored lenses, and an environment closer to the natural environment is created. According to the present invention, with the arrangement of the CCD camera, the weak fluorescence signal can be captured, the specific gene of the living plant can be subjected to fluorescence labeling, and after the EMS mutagenesis treatment, the fluorescence brightness change can be detected in the imaging device so as to find the enhancer or the suppressor, such that the biological research data can be obtained; and a better use prospect can be brought.

1. The name of the design product: animal live imaging instrument. 2. The use of the design product: for live small animal imaging. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The present invention discloses an Airy two-photon microscopy imaging method and device with global enhanced aberration correction, belonging to the technical field of biological imaging. The imaging method includes the following steps: S1. Expand the laser beam through a laser beam expansion module to ensure that the laser illumination beam meets the beam size requirements for subsequent modulation; S2. Generate an Airy beam and perform wavefront correction through an AO correction module; S3. Split and combine two beams through a beam splitting and combining module; S4. Drive the beam to scan the entire sample through a beam scanning module; S5. Collect the sample signal through a fluorescence collection module. By adopting the above-mentioned Airy two-photon microscopy imaging method and device with global enhanced aberration correction, the present invention simultaneously realizes the generation of an Airy beam and real-time aberration correction through a single spatial light modulator, improves the imaging resolution of the two-photon imaging device, and helps to observe more detailed tissue information in in vivo imaging applications.

The invention provides a high-sensitivity oxidative stress near-infrared fluorescent probe for in-vivo imaging and application thereof, the near-infrared fluorescent probe is a compound shown as a formula I, R1 and R2 are defined in the description, and the absorption and emission wavelength of the fluorescent compound obtained after the near-infrared fluorescent probe is activated by active oxygen is near 890 nm and even larger than 890 nm. The near-infrared fluorescent probe disclosed by the invention shows rapid and relatively high reactivity to active oxygen, and the generated fluorescent compound is high in fluorescence intensity and good in stability and can be used for monitoring the active oxygen level in a living body. # imgabs0 #

The invention provides an anti-tumor combined treatment method for improving a radiotherapy curative effect by using nintedanib, and relates to the technical field of tumor treatment. The anti-tumor combined treatment method comprises the following steps: selecting a male mouse or a male nude mouse as an experimental animal, and subcutaneously constructing a tumor-bearing mouse model on the back of the experimental animal; when the tumor volume of the tumor-bearing mice reaches 70-100mm < 3 >, randomly dividing the tumor-bearing mice into four groups, namely a control group, a nintedanib single drug group, a radiotherapy group and a combined group; measuring the subcutaneous tumor volume and weight of the tumor-bearing mouse every three days; drawing a mouse tumor growth curve, a mouse survival curve and a mouse weight change curve according to the measured data; a tumor-bearing mouse is subjected to living body imaging, tumor tissue paraffin embedding, immunohistochemical staining, TUNEL staining and Hamp; and E, carrying out section staining and blood biochemical detection, and carrying out data analysis by utilizing corresponding analysis software, so as to evaluate the anti-tumor effect and safety of the combined treatment. According to the invention, tumor growth can be effectively inhibited.

1. Name of the product in this design: Live Imaging Organ Fixation Instrument. 2. Purpose of this design: To fix organs or tissues of live experimental animals during microscopic imaging. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present invention belongs to the field of medicine, and particularly relates to a CXCR4-targeting polypeptide and its application. The present invention first provides a CXCR4-targeting polypeptide, which is selected from any one of SEQ ID NO.1-10; then provides a molecular probe, which is composed of a fluorescent label or a radionuclide conjugated to the above-mentioned CXCR4-targeting polypeptide; finally, it also provides the use of any one of the above-mentioned CXCR4-targeting polypeptides and any one of the above-mentioned molecular probes in the preparation of reagents for cancer diagnosis or treatment. The CXCR4-targeting polypeptide provided by the present invention can specifically target CXCR4, conjugate with a fluorescent label or a radionuclide, and achieve high-sensitivity in vivo imaging of tumors; it can be used for tumor screening and early diagnosis, and can also realize the treatment monitoring of tumors. The polypeptide of the present invention has good biocompatibility and safety. The polypeptides of the present invention are all reported for the first time, and are easy to obtain and convenient to promote.

The invention discloses a formaldehyde probe as well as a preparation method and application thereof, and belongs to the technical field of fluorescent probe preparation. Dissolving benzylamine and potassium carbonate in anhydrous acetonitrile, adding 2-bromo-2-methyl propionate, and reacting to obtain a compound 3; dissolving the compound 3 and lithium hydroxide in a mixed solution of tetrahydrofuran and water, reacting for a period of time, and quenching the reaction with hydrochloric acid to obtain a carboxylic acid intermediate 4; and dissolving the carboxylic acid intermediate 4 in dichloromethane, then adding CDM, EDCI and DMAP, and carrying out a reaction to obtain the target product DPM. The DPM is a formaldehyde regeneration type fluorescent probe and can realize formaldehyde detection in a mode of disturbing an endogenous steady state to the minimum extent. The DPM is a steady-state maintenance type formaldehyde probe successfully applied to a spinal cord injury (SCI) model for the first time, and can realize visual monitoring on formaldehyde rise of a diseased region based on a non-invasive living imaging system (IVIS).

The application discloses an electron acceptor, a fluoroborondiphenylamine fluorescent dye and a preparation method and application thereof, wherein, the difluoroboron bridged difluoroboron pseudoindole electron acceptor is easy to synthesize, and can be further condensed with a Fischer aldehyde or a derivative thereof to obtain a series of fluoroborondiphenylamine fluorescent dyes covering far infrared to near infrared, which effectively enriches the types of fluorescent dyes, and the application further provides the fluoroborondiphenylamine fluorescent dye which can be used for lipid droplet fluorescent imaging, and the effects of high-efficiency lipid droplet targeting, high signal-to-noise ratio lipid droplet imaging and multi-color (multi-channel) fluorescent lipid droplet imaging are achieved, wherein, the near-infrared lipid droplet probe has better application prospect in tissue and living body imaging.

The present invention discloses a humanized animal model for human influenza virus infection and a preparation method. The present invention provides a method for constructing a mouse model for human influenza virus infection, comprising the following steps: introducing the endothelial cell-specific promoter K18, the human sialyltransferase ST6GAL1 encoding gene, and the luciferase encoding gene into wild-type mice; subsequently breeding to obtain the F2 generation, which is a mouse model for human influenza virus infection; and each generation is subjected to genotyping and in vivo imaging identification. The present invention inserts the human ST6GAL1 gene into the mouse gene sequence, overexpresses the human sialyltransferase ST6GAL1 that synthesizes the human α2,6-linked sialic acid receptor, and constructs a mouse model for human influenza virus infection. The model can be directly infected with human influenza virus without the need for an in vivo adaptation process in mice, providing a humanized mouse model that conforms to the clinical characteristics of influenza for in-depth elucidation of the pathogenesis of influenza, verification of new drug targets, and development of new drugs and vaccine evaluation.

This utility model discloses a large-spot laser application device, including a device box, and an angle adjustment plate, a laser light source, and a projection plate disposed inside the device box. The two angle adjustment plates are respectively disposed on both sides above the projection plate, and a laser light source is installed on each of the two angle adjustment plates. The irradiation direction of the laser light source is aligned with the projection plate, and the laser light source and the angle adjustment plate are connected to form an adjustable irradiation angle structure. This utility model uses a laser light source to replace the traditional LED light source, halogen light source, and xenon lamp light source, which not only extends the service life of the light source, but also makes the light source smaller and more conducive to the integration of the entire structure. It can be directly applied to the excitation of light sources in CCD camera multicolor fluorescence chemiluminescence gel imaging systems and animal and plant live imaging systems without replacing the light source.

This application discloses an optical in vivo imaging device and method for animal spleen, belonging to the field of biomedical devices and instruments. The imaging device includes a first imaging block and a second imaging block; the first imaging block is provided with a first notch recessed inward from its end face, and the top view projection of the first notch includes a first arc; the second imaging block is provided with a second notch recessed inward from its end face, and the top view projection of the second notch includes a first straight line, a second arc, and a second straight line connected in sequence; the first imaging block is fitted and snapped into the second imaging block so that the first and second notches form a closed imaging window with an adjustable imaging area. This application has a large effective imaging area, enabling full-field, highly stable imaging of the spleen, and 100% observation of all microstructures of the spleen. It can image the marginal areas and white pulp areas located in deeper positions, and can achieve cross-regional tracking observation.

This invention relates to a medaka hatching enzyme, its preparation method, and its application. The preparation method of the medaka hatching enzyme includes the following steps: 1) collecting fertilized medaka eggs; 2) incubating the fertilized medaka eggs with embryo culture medium in a 26℃ incubator for 6 days, and collecting mature embryos; 3) adding NaCl solution and Tris-HCl buffer (pH=8.0) to the mature embryos, grinding them, collecting the filtrate, and allowing it to stand overnight at 4℃; 4) centrifuging the filtrate at 15000 rpm for 15 minutes, and extracting the supernatant to obtain the medaka hatching enzyme solution. The preparation method is simple to operate, and the obtained medaka hatching enzyme has high activity, which can help to quickly remove the medaka eggshell artificially. The naked medaka embryos obtained by this method can be directly used for in vivo imaging and in-situ quantitative analysis, and embryo model construction, providing support for subsequent genetic, developmental biological, and toxicological studies of medaka embryos.

The present invention provides a probe for in vivo imaging, its preparation method, and its application, relating to the field of molecular probes. The probe for in vivo imaging proposed by the present invention comprises: a fluorescent protein unit, a contrast agent unit, and a targeting unit; wherein the fluorescent protein unit, contrast agent unit, and targeting unit are connected by a linker. The probe proposed by the present invention is a novel magnetic resonance probe targeting PD-L1, capable of noninvasive and radiation-free molecular imaging, and has tumor therapeutic effects.

This application provides a ratiometric fluorescent probe for detecting divalent copper ions, its preparation method, and its application. The ratiometric fluorescent probe comprises fluorescently labeled human serum albumin with isothiocyanate and a dye compound; the fluorescently labeled human serum albumin with isothiocyanate is coated on the surface of the dye compound; the dye compound is a compound with the chemical formula shown in Formula I, II, III, or IV. The ratiometric fluorescent probe provided in this application can be applied to various Cu... 2+ The detection scenario, and can detect Cu 2+ Quantitative detection, on-site rapid testing, and live imaging analysis are performed.

Providing imaging equipment 2 capable of obtaining high-precision images within living organisms. [Solution] The imaging device 2 has a subshaft 6, a first cable 8a, a second cable 8b, and a head 10. The head 10 has a cylinder 12, a filler 14, a cantilever 31, a guide 18, a camshaft 20, and a cam 22. The cantilever 31 includes a first sensor 30a and a second sensor 30b. The guide 18 has a guide groove 32. The cantilever 31 is inserted into this guide groove 32. The rotation of the subshaft 6 causes the cam 22 to rotate. The rotation of the cam 22 causes the cantilever 31 to reciprocate while being guided by the guide groove 32.

The present invention discloses a small animal live imaging device and method. The small animal live imaging device includes: a stage with multiple light-transmitting windows for placing the small animal; a CMOS camera disposed above the stage; a brightfield light source disposed above the stage; a fluorescent light source assembly disposed above the stage; a filter wheel rotatably disposed below the CMOS camera, with multiple filters that transmit different wavelengths and at least one through-hole disposed on the same circumference of the filter wheel; a large-scale image sensor disposed below the stage and used for upward imaging; a vertical movement device disposed below the stage and used to drive the large-scale image sensor to move vertically; and a horizontal movement device disposed below the stage and used to drive the vertical movement device and the large-scale image sensor to move horizontally. In the present invention, fluorescent probe imaging and luminescent probe imaging of small animals can be achieved using the CMOS camera and the large-scale image sensor, respectively.

The utility model relates to the technical field of animal living body imaging, in particular to an automatic focusing small animal living body imager which comprises a cabinet body, a cabinet door is hinged to the bottom of the cabinet body through a hinge piece, a lifting module is arranged at the bottom end in the cabinet body, and a medicine box is placed on the surface of the lifting module. The lifting module is used for driving the medicine box to move up and down; an inner clamping plate is further fixed to the top end in the cabinet body, a radiator is fixedly connected to the surface of the inner clamping plate, and heat dissipation fans are embedded into the two ends of the rear side of the cabinet body and matched with the radiator to conduct heat dissipation work on the instrument; the bottom of the inner clamping plate is further rotationally connected with a lens, and a focusing mechanism is further arranged on the surface of the lens and used for focusing the lens. According to the utility model, the automatic focusing function is realized when the imager is used, so that the convenience of the imager in use is improved.

The present invention discloses a class of selenocyanine compounds having the following structure: wherein R represents -OH, -NH2 or N,N-diethylamino; X ‑ Represents anion. The absorption and emission wavelengths of the selenocyanine compounds of the present invention are both located in the near-infrared region (700nm-900nm). They effectively target mitochondria and can be used as fluorescent dyes for cell and in vivo imaging and tumor-targeted photodynamic / photothermal combined therapy.

The present application relates to the technical field of imaging, in particular to an optical coherence elastography method and device, electronic equipment and storage medium, wherein the method comprises: outputting laser light meeting preset time periodicity conditions and preset linear variation conditions by using a sweep frequency light source; generating a driving signal containing an analog signal or a digital signal according to a trigger signal; scanning a sample according to the driving signal; applying mechanical excitation to the sample by using the driving signal, generating two-dimensional imaging and three-dimensional imaging of the sample according to two-dimensional elastic waves and three-dimensional elastic waves respectively; interfering laser light reflected by a sample arm with reference arm laser light; reconstructing the interference signal into a sample structure image, generating biomechanical information of the sample according to the elastic waves, and determining the final optical coherence elastography result according to the biomechanical information, the three-dimensional imaging and the two-dimensional imaging. Thus, the problem that the existing optical coherence elastography method cannot realize two-dimensional dynamic imaging and three-dimensional in vivo imaging is solved.

The invention relates to the technical field of supramolecular chemistry, in particular to a tetradentate pyridine ligand, a near-infrared two-region fluorescent metal cage as well as a preparation method and application of the tetradentate pyridine ligand and the near-infrared two-region fluorescent metal cage. The structural formula of the tetradentate pyridine ligand is shown in the specification, R is shown in the specification, and the element X is S or Se. The tetradentate pyridine ligand disclosed by the invention is a typical tetradentate pyridine ligand with a D-pi-A-pi-D structure and has strong near-infrared absorption capacity and efficient near-infrared two-region emission, and absorbed photon energy can be released in a fluorescent form; therefore, the metal cage prepared from the metal nano-material can be applied to in-vivo near-infrared two-region fluorescence imaging of mouse tumors. Due to the small ground state-excited state energy gap difference of the tetradentate pyridine ligand, photon energy absorbed by the metal cage prepared from the tetradentate pyridine ligand can also be released in the form of non-radiative transition, namely heat, so that the metal cage can be further applied to photo-thermal imaging and photo-thermal treatment and can play a complementary role with near-infrared two-region fluorescence imaging, and the in-vivo imaging quality is improved.