An infection method for pathogenicity identification of sugarcane leaf fungal disease strains

Abstract

The invention relates to the technical field of sugarcane disease inspection, and provides an infecting method for authenticating the pathogenicity of sugarcane leaf fungal disease strains. The method comprises the steps of preparation of strains, collection and pretreatment of leaves, preparation of infected leaves, infection, culture and pathogenicity evaluation of strains. The method is characterized in that the preparation step of the infected leaves comprises the substeps: shearing the leaves into long segments of 4-8cm, wrapping two ends of the leaves with tissues, putting the leaves into a sterilized culture dish, and dripping sterilized clean water onto the tissues at the two ends of the leaves; and the infection step comprises the substeps: pricking two sides of the midribs of the leaves in the culture dish by using a pricking device, clipping hyphae to the pricked positions of the leaves by using sterilized tweezers to serve as a test culture dish. The method has the following predominant substantial characteristics and significant progresses: (1) a few sugarcane leaves and hyphae need to be infected; (2) the temperature and humidity of the infected sugarcane leaves can be controlled, so as to meet the requirements of pathogenic bacteria attack for high temperature and high humidity; and (3) the infected strains with pathogenicity are attacked significantly and have high sensitivity.

Description

technical field [0001] The invention relates to the technical field of sugarcane disease inspection, in particular to an infection method suitable for evaluating the pathogenicity of sugarcane leaf fungal disease strains. Background technique [0002] In recent years, with the development of the sugar cane industry, the situation of sugarcane planting showing a single variety and large-scale continuous planting has become more and more obvious, and the accompanying disease problems have become increasingly prominent, especially sugarcane leaf diseases. According to incomplete statistics, there are nearly 30 kinds of diseases (collectively referred to as leaf diseases) that occur on sugarcane leaves, most of which are fungal diseases. Due to the accumulation of pathogenic bacteria year by year, the occurrence of sugarcane leaf diseases is particularly prominent in recent years. The photosynthesis of diseased plants is affected, which hinders the growth of sugarcane plants, sh...

AI Technical Summary

Problems solved by technology

Since sugarcane is a relatively large crop, it is difficult to control its temperature and humidity during artificial infection in the field. When there are many infected strains, it takes a lot of manpower and material resources.

Appropriate temperature and humidity are necessary conditions for the disease to occur. If the temperature and humidity cannot be controlled, the authenticity, stability and accuracy of the infection results cannot be guaranteed.

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