Tumor chemotherapy drug susceptibility detection chip and using method thereof

Abstract

The invention discloses a tumor chemotherapy drug susceptibility detection chip and a using method thereof. The tumor chemotherapy drug susceptibility detection chip is formed by sealing a shell with a chip microstructure on the inner surface and a bottom carrier, wherein the chip microstructure comprises a nutrient solution perfusion unit, four cell culture units and a biological glue filling unit. According to the tumor chemotherapy drug susceptibility detection chip, multiple cell ingredients in in-vitro tumor tissue can be integrated on one micro chip for co-culture, biological factors generated by multiple cells can be diffused to the whole micro platform, heterocytotropic cells are ensured not to be contacted mutually, and the in-vivo growth microenvironment of the in-vitro tumor cell can be simulated to the maximum extent.

Description

technical field [0001] The invention relates to a tumor chemotherapeutic drug sensitivity detection chip based on microfluidic technology and an application method thereof, belonging to the field of biomedical research. Background technique [0002] Cell biology research is developing from the observation and study of a single cell to the observation and study of the interaction of two or even multiple types of cells. For complex biological systems, a system in which multiple cells exist at the same time can better simulate the real cellular microenvironment, thereby more comprehensively and deeply exploring the complex network between cells and the signaling pathways that mediate their interactions, etc. , It is of great significance to the occurrence and development of tumors and the sensitivity to chemotherapeutic drugs. Cell co-cultivation is a common method for studying cell-cell interactions. [0003] At present, although the conventional mixed culture of cells can s...

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Problems solved by technology

[0003] At present, although the conventional mixed culture of cells can solve the problem of multi-cell interaction in vitro, and at the same time play a role in simulating the in vivo environment to a certain extent, it is necessary to use a large Equipment (such as flow cytometry) for separation and identification is expensive, cumbersome, and limited in clinical application

At present, microfluidic chips based on cell culture are mostly limited to the co-cultivation of two types of cells, or the mixed culture of one target cell and other types of cells. The cell components in the microenvironment are observed, and the sensitivity detection of chemotherapy drugs is performed on the cells in this state, which also limits the accuracy of the result judgment

In addition, the culture chamber perfusion channel of the microfluidic chip currently used for drug screening is mostly two relatively independent and parallel channels, which limits the contact area between the channel and the cell culture chamber; and the cell culture chamber and the nutrient solution perfusion channel are mostly direct. Contact, cells easily diffuse into the perfusion channel, which limits the large-scale clinical application

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