A traditional Chinese medicine perfusate for promoting uterine contraction and content discharge of sows and its application
The technology of uterine contraction and perfusion is applied in the field of traditional Chinese medicine perfusion to promote the uterine contraction of sows and the discharge of contents, which can solve the problems of high intensity of action, narrow application range of oxytocin, and influence on normal reproduction and production of sows. To achieve the effect of promoting excretion and preventing endometritis
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Embodiment 1
[0025] Accurately weigh and air-dry 250g of Hedyotis diffusa 250g, 250g of Desmodium, 200g of Duckweed, 120g of cassava, and 300g of Hedyotis diffusa, chop them up and mix them through an 80-mesh sieve, put them into an ultrasonic container, add 15kg of absolute ethanol Extract under ultrasonic state for 1h, ultrasonic power 40W, filter, the filtrate is evaporated and dried at 50°C until the mass is 3%-5% of the original drug, to obtain the extract, weigh the quality of the extract, and prepare 14 times the mass of the extract in addition normal saline and dehydrated alcohol weighing 6 times the quality of the extract to prepare a dilution solution, put the extract into the dilution solution, fully dissolve and shake well to obtain the perfusion solution, and use 100ml glassware to subpackage the perfusion solution. Seal it, steam sterilize it at 105°C for 10 minutes, and leave it to cool to get the finished product.
Embodiment 2
[0027] Accurately weigh and air-dry 300g of ghost needle grass, 300g of money grass, 250g of duck grass, 100g of cassava, and 400g of hedyotis diffusa, chop them up and mix them through an 80-mesh sieve, put them into an ultrasonic container, add 25kg of absolute ethanol Extract under ultrasonic state for 2h, ultrasonic power 40W, filter, the filtrate is evaporated and dried at 50°C until the mass is 3%-5% of the original drug, and the extract is obtained, and the mass of the extract is weighed, and 40 times the mass of the extract is prepared in addition normal saline and absolute ethanol weighing 10 times the quality of the extract to prepare a dilution solution, put the extract into the dilution solution, fully dissolve and shake well to obtain the perfusion solution, and use 100ml glassware to subpackage the perfusion solution. Seal it, steam sterilize it at 105°C for 10 minutes, and leave it to cool to get the finished product.
Embodiment 3
[0029] Contraction of perfusate on isolated mouse uterus
[0030] 1. Experimental method: kill a group of young mice after inducing estrus, separate the uterine horns and cut off 1cm long fragments, place them in a tissue bath filled with 10ml buffer solution, add the perfusate of the present invention in the container, Observe the strength and frequency of uterine contractions, and record them with a load cell. Acetylcholine is used as a positive control, and ethanol is used as a blank control.
[0031] 2. Experimental results:
[0032] As a result, it was not observed that ethanol could cause uterine contraction, but the perfusate of the present invention caused obvious contraction of mouse uterine tissue, and the contraction intensity was 23% higher than that of acetylcholine, and the frequency was increased by 56%.
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