Bacillus subtilis strain, biocontrol microbial agent and preparing method and application of bacillus subtilis strain and biocontrol microbial agent

Abstract

The invention belongs to the technical field of biological material preparation and provides a bacillus subtilis wdcjxq2266, a biocontrol microbial agent produced from the strain and application of the strain and the biocontrol microbial agent. The bacillus subtilis wdcjxq2266 is preserved in the General Microorganism Center of China Microbiological Culture Collection and Management Committee at April third, 2014, and the culture preservation number is CGMCC NO.8993. The strain is cultured in an LB culture solution in a shaking way at the temperature of 28 DEG C and the speed of 180 rpm for 12-24 hours, a microbial agent finished product with a total viable bacteria concentration of 1*109-1*1010 CFU / mL is prepared, and after being diluted for 10-100 times, the microbial agent finished product is used for seed soaking treatment during pepper planting or root irrigating treatment during transplanting. The bacteriostasis effect is very remarkable. Tests verify that the pepper disease preventing rate of the biocontrol microbial agent can reach 69.06% under room temperature conditions, and the pepper yield increase range reaches 38.5%.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a bacillus subtilis wdcjxq2266, a biocontrol agent produced by the strain, and an application of the strain and the biocontrol agent in preventing and treating pepper blight. Background technique [0002] Pepper blight is a bacterial disease caused by Phytophthora capsici Leonian. Phytophthora capsici has a wide range of hosts, and in addition to peppers, it can also parasitize tomatoes, eggplants and some melon crops. The fungus survives the winter as oospores in the soil or diseased debris, and is spread by wind, rain, irrigation and other agricultural activities. After the onset, new sporangia can be produced to form zoospores for reinfection. The germ growth temperature ranges from 10 to 37°C, and the optimum temperature is 20 to 30°C. When the relative humidity of the air reaches above 90%, the onset is rapid; heavy cropping, low-lying land, poor drainage, excessiv...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the prevention and control effect of the preparation for preventing and treating pepper blight in the prior art is unsatisfactory, and it is easy to cause environmental pollution. A kind of Bacillus subtilis bacterial strain, a biocontrol bacterial agent comprising the bacterial strain and the preparation of the bacterial agent are provided. And the application of the two in the prevention and treatment of pepper blight

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