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Use of Pinus yunnanensis Fr. pinecone extract in preparation of antitumor drugs

The technology of extract and pine pine tower is applied in the field of preparing anti-tumor drugs, and achieves the effects of simple extraction process, abundant and easy-to-obtain resources, and low cost.

Inactive Publication Date: 2015-03-11
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of the novelty search showed that there were no previous reports on the preparation of anti-tumor drugs using the extracts of Songta pine cones in Yunnan as the main drug after water extraction and alcohol precipitation

Method used

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  • Use of Pinus yunnanensis Fr. pinecone extract in preparation of antitumor drugs
  • Use of Pinus yunnanensis Fr. pinecone extract in preparation of antitumor drugs
  • Use of Pinus yunnanensis Fr. pinecone extract in preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 : Cytotoxic activity test of Yunnan pine cone extract on human liver cancer cells (HepG-2 cell line)

[0018] 1.1 Preparation of Yunnan pine cone extract:

[0019] Yunnan pine cones were collected from Yangbi County, Dali Bai Autonomous Prefecture, Yunnan Province. It was identified as cones of Pinus yunnanensis Fr. by Professor Xia Conglong from the Department of Pharmacognosy, Dali College. The Yunnan pine cones were dried in the sun for later use.

[0020]10 kg of dried pine cones of Yunnan pine, crushed, soaked in 20 liters of 95% ethanol solution for 24 hours, repeated 3 times, and filtered. The residue was extracted with boiling water for 2 hours, repeated 3 times; then extracted with 1% sodium hydroxide solution at room temperature, and filtered. The filtrate was adjusted to pH 5 with 10% acetic acid solution, and filtered. The filtrate was precipitated with 1 times the amount of 95% ethanol solution, and filtered to obtain precipitate D. The filtra...

Embodiment 2

[0038] Example 2 : Cell Growth Inhibitory Test of Yunnan Pine Cone Extract on Human Hepatoma Cells (Huh-7 Cell Line)

[0039] 2.1 Preparation of Yunnan pine cone extract:

[0040] The same method as in Example 1.1 was used to prepare the pine cone extract of Yunnan pine cone (sample number: YNS).

[0041] 2.2 Cytotoxicity test:

[0042] 2.2.1 Instruments and materials

[0043] (1) Instrument: with embodiment 1.

[0044] (2) material: with embodiment 1

[0045] (3) Cell line: human liver cancer cell line Huh-7, purchased from the Cell Bank of Kunming Institute of Zoology, Chinese Academy of Sciences.

[0046] 2.2.2 Method

[0047] (1) Cell inoculation: Take Huh-7 cells, culture them in DMEM medium containing 10% inactivated FBS in a 37°C, 5% carbon dioxide incubator, take the above cells in the logarithmic growth phase, and digest them with 0.25% trypsin After centrifugation, the supernatant was discarded; the cell concentration was adjusted to 1×10 with complete medium...

Embodiment 3

[0058] Example 3 : The effect of Yunnan pine cone extract on normal liver cells (L-02)

[0059] 3.1 Purpose of the experiment:

[0060] The toxicity of the extract was evaluated through the growth effect of the extract from the pine cone of Yunnan pine cone on the growth of normal liver cells (L-02 cell line).

[0061] 3.2 Preparation of Yunnan pine cone extract:

[0062] The same method as in Example 1.1 was used to prepare the pine cone extract of Yunnan pine cone (sample number: YNS).

[0063] 3.3 Cytotoxicity test:

[0064] 3.3.1 Instruments and materials

[0065] (1) Instrument: with embodiment 1.

[0066] (2) Material: with embodiment 1.

[0067] (3) Cell line: normal human hepatocyte L-02, purchased from the Cell Bank of Kunming Institute of Zoology, Chinese Academy of Sciences.

[0068] 3.3.2 Method

[0069] (1) Cell inoculation: L-02 cells were cultured in DMEM medium containing 10% inactivated FBS in a 37°C, 5% carbon dioxide incubator, and the above-mention...

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Abstract

The invention relates to a use of Pinus yunnanensis Fr. pinecone extract in preparation of antitumor drugs. Concretely, the invention relates to an effective part prepared from pinecone of pinaceae pinus plant Pinus yunnanensis Fr. by ethanol solution immersion, hot water extraction, alkaline water extraction at a room temperature and alcohol precipitation extraction on the filtrate obtained by the alkaline water extraction, and also relates to a use of the effective part in preparation of antitumor drugs. The effective part has a function of obviously inhibiting human liver cancer cell proliferation, has small toxicity to human normal hepatocytes and can be developed to form a drug for preventing and treating tumor diseases.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the invention relates to the medical application of the pine cone extract of the genus Yunnan Pinus for preparing antitumor drugs. The pine cone extract is obtained from the cones of Pinus yunnanensis Fr. soaked in ethanol solution, extracted in hot water, extracted with alkaline water at room temperature, and then extracted by alcohol precipitation. It has cytotoxic activity and anti-inflammatory properties. Tumor function can be expected to be developed into a drug for preventing and treating tumor diseases. Background technique [0002] In recent years, with the improvement of living standards, the change of ecological environment and the increase of life pressure, cancer has become an upward trend. Among them, liver cancer is one of the common malignant tumors in my country and the third leading cause of cancer death in the world. It is highly malignant, progresses rapidly, a...

Claims

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Application Information

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IPC IPC(8): A61K36/15A61P35/00A61K131/00
CPCA61K36/15A61K2236/33A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 李春艳刘熙李冬梅于冬梅彭友伦施贵荣杨敏张兰胜巫秀美
Owner DALI UNIV
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