Spore suspension for improving yield of white muscardine silkworms and application of spore suspension

Abstract

The invention discloses spore suspension for improving the yield of white muscardine silkworms and application of the spore suspension. The spore suspension for improving the yield of the white muscardine silkworms comprises the following raw materials: muscardine spore powder with 1x106-5x106 muscardine spores per mL, 0.2 part by mass of polysorbate-80, 1-3 parts by mass of cane sugar, 0.4-0.6 part by mass of peptone, 0.05 part of auxiliary materials and the balanced sterile water. The auxiliary materials can be magnesium sulfate, chloramphenicol and streptomycin sulfate. By the spore suspension for improving the yield of the white muscardine silkworms, the concentration of the optimized muscardine suspension is increased, the stickiness and the activity of the muscardine suspension are improved, the germination rate of the muscardine suspension is increased, and the yield and the quality of the white muscardine silkworms are improved obviously.

Description

technical field

[0001] The invention belongs to the technical field of traditional Chinese medicine production, and in particular relates to a spore suspension for improving the yield of silkworm Bombyx basilisk and an application thereof. Background technique

[0002] Silkworm is an excellent Chinese medicinal material, which has the effects of relieving wind and spasm, dispelling wind and relieving pain, resolving phlegm and resolving stagnation, and is widely used in Chinese patent medicines and prescriptions. According to statistics, 49 out of 513 kinds of patent medicines for human use contain silkworm, and 29 kinds of 64 kinds of patent medicines for children contain silkworm. With the in-depth research on the pharmacological components and functions of Bombyx mori, it was found that the water extract or alcoholic extract of Bombyx mori also showed good effects in whitening and freckle removal, anticonvulsant, anticoagulant, and hypoglycemic and lipid-lowering. In rec...

Examples

Embodiment 1

[0021] The raw materials and their proportions of this embodiment include: Beauveria bassiana spores 3×10 6 per mL, 0.2 parts of polysorbate-80 (mass percentage), 2 parts of sucrose, 0.5 parts of peptone, 0.05 parts of excipients, and the rest is sterile water.

[0022] The inoculation steps are as follows: (1) Select a certain species of silkworm for feeding, and raise it to the fifth instar silkworm stage after the fourth molting (before eating mulberry). After 2 hours, select it and enter the follow-up treatment. Head silkworm; (2) Configure the spore suspension in the above method, and place the spore suspension in the sprayer; (3) Spray the spore suspension on the body surface of the silkworm until the body surface of the silkworm is completely wet, and after one hour, spray The treated silkworms continued to be reared with mulberry leaves, and the breeding environment was controlled to be at a suitable temperature and humidity, and the death situation was investigated ev...

Embodiment 2

[0024] The raw materials and their proportions of this embodiment include: Beauveria bassiana spores 3×10 6 pcs / mL, polysorbate-80 0.2 part (mass percentage), and the rest is sterile water.

[0025] The inoculation steps are as follows: (1) Select a certain species of silkworm for feeding, and raise it to the fifth instar silkworm stage after the fourth molting (before eating mulberry). After 2 hours, select it and enter the follow-up treatment. Head silkworm; (2) Configure the spore suspension in the above method, and place the spore suspension in the sprayer; (3) Spray the spore suspension on the body surface of the silkworm until the body surface of the silkworm is completely wet, and after one hour, spray The treated silkworms continued to be reared with mulberry leaves, and the breeding environment was controlled to be at a suitable temperature and humidity, and the death situation was investigated every day within 7 days; (4) The number of dead silkworms was recorded eve...

technical field

[0001] The invention belongs to the technical field of traditional Chinese medicine production, and in particular relates to a spore suspension for improving the yield of silkworm Bombyx basilisk and an application thereof. Background technique

[0002] Silkworm is an excellent Chinese medicinal material, which has the effects of relieving wind and spasm, dispelling wind and relieving pain, resolving phlegm and resolving stagnation, and is widely used in Chinese patent medicines and prescriptions. According to statistics, 49 out of 513 kinds of patent medicines for human use contain silkworm, and 29 kinds of 64 kinds of patent medicines for children contain silkworm. With the in-depth research on the pharmacological components and functions of Bombyx mori, it was found that the water extract or alcoholic extract of Bombyx mori also showed good effects in whitening and freckle removal, anticonvulsant, anticoagulant, and hypoglycemic and lipid-lowering. In rec...

Examples

Embodiment 1

[0021] The raw materials and their proportions of this embodiment include: Beauveria bassiana spores 3×10 6 per mL, 0.2 parts of polysorbate-80 (mass percentage), 2 parts of sucrose, 0.5 parts of peptone, 0.05 parts of excipients, and the rest is sterile water.

[0022] The inoculation steps are as follows: (1) Select a certain species of silkworm for feeding, and raise it to the fifth instar silkworm stage after the fourth molting (before eating mulberry). After 2 hours, select it and enter the follow-up treatment. Head silkworm; (2) Configure the spore suspension in the above method, and place the spore suspension in the sprayer; (3) Spray the spore suspension on the body surface of the silkworm until the body surface of the silkworm is completely wet, and after one hour, spray The treated silkworms continued to be reared with mulberry leaves, and the breeding environment was controlled to be at a suitable temperature and humidity, and the death situation was investigated ev...

Embodiment 2

[0024] The raw materials and their proportions of this embodiment include: Beauveria bassiana spores 3×10 6 pcs / mL, polysorbate-80 0.2 part (mass percentage), and the rest is sterile water.

[0025] The inoculation steps are as follows: (1) Select a certain species of silkworm for feeding, and raise it to the fifth instar silkworm stage after the fourth molting (before eating mulberry). After 2 hours, select it and enter the follow-up treatment. Head silkworm; (2) Configure the spore suspension in the above method, and place the spore suspension in the sprayer; (3) Spray the spore suspension on the body surface of the silkworm until the body surface of the silkworm is completely wet, and after one hour, spray The treated silkworms continued to be reared with mulberry leaves, and the breeding environment was controlled to be at a suitable temperature and humidity, and the death situation was investigated every day within 7 days; (4) The number of dead silkworms was recorded eve...

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