A kind of primer and method for detecting single nucleotide polymorphism of goat dqa1 gene exon 4

A single nucleotide polymorphism, gene technology, applied in the field of molecular genetics

Active Publication Date: 2018-06-05
广西平果润民发展有限公司
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Problems solved by technology

However, there are few domestic reports on the immune traits of Guizhou white goats. Therefore, how to use molecular biology techniques to quickly and efficiently screen and breed Guizhou white goats with high disease resistance has become the top priority for improving my country's excellent local goat breeds. Heavy

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  • A kind of primer and method for detecting single nucleotide polymorphism of goat dqa1 gene exon 4
  • A kind of primer and method for detecting single nucleotide polymorphism of goat dqa1 gene exon 4
  • A kind of primer and method for detecting single nucleotide polymorphism of goat dqa1 gene exon 4

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Embodiment

[0028] The principles and features of the present invention will be described below in conjunction with specific embodiments, which are only used to explain the present invention, and are not intended to limit the scope of the present invention. Taking the polymorphism analysis of exon 4 of the immune traits-related gene DQA1 gene of Guizhou white goat as an example, the content of the present invention will be described in detail below.

[0029] A Guizhou white goat DQA1 Exon4 allele PCR-SSCP detection method, its main feature is that the specific detection steps are as follows:

[0030] 1. Sample collection: Collect adult Guizhou white goats in good health, collect 8 mL of blood from the jugular vein with an anticoagulant tube, bring the ice box back to the laboratory, and store it at -20°C;

[0031] 2. Genomic DNA extraction: Genomic DNA was extracted using the Blood Genome Extraction Kit (Shanghai Sangon Biotech);

[0032] 3. Primer design and PCR reaction: the total syst...

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Abstract

The invention discloses a primer and method for detecting the mononucleotide polymorphism of a DQA1 gene related to goat immunity. The primer comprises an upstream primer and a downstream primer. When detection is carried out, goat genome DNA is taken as a template, PCR (polymerase chain reaction) is carried out by utilizing the upstream primer and the downstream primer to amplify a target fragment, a PCR product is treated by using a denaturing agent, the denatured amplified fragment is subjected to non-denatured polyacrylamide gel electrophoresis detection, glue dyeing is carried out by applying a silver dyeing method, and an allelic gene of a goat DQA1 gene exon 4 can be judged according to a polyacrylamide gel electrophoresis result. According to the nucleotide primer disclosed by the invention and an established detection system, different allelic genotypes of a group can be more accurately and faster amplified, and different mononucleotide mutant sites of the goat DQA1 gene exon 4 can be obtained through accurate analysis.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to goat molecular marker assisted breeding technology, in particular to a primer and a method for detecting the single nucleotide polymorphism of goat immune-related gene DQA1 gene. Background technique [0002] The goat DQA1 gene is located in the DQ subregion of the MHC class Ⅱ gene and has abundant polymorphisms. MHC (major histocompatibility complex, major histocompatibility complex) is a multi-gene family related to immunity in vertebrates. It has genetic characteristics such as co-dominance and linkage disequilibrium. Protein that participates in the regulation of immune responses by delivering antigens to T lymphocytes. MHC genes are composed of class I, II and III genes. The MHC gene of goat is called GOLA (Goat lymphocyte antigen), that is, goat leukocyte antigen. MHC gene polymorphism endows animals with great adaptability. Long-term natural selection makes animals have the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q2531/113C12Q2565/131
Inventor 罗卫星刘彬蔡慧芬康建兵孙岩岩钱成
Owner 广西平果润民发展有限公司
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