Method for separating and purifying anthocyanins from red-fleshed peaches

A technology for anthocyanins and red peaches, which is applied in material separation, chemical instruments and methods, and analytical materials, and can solve the problems of inability to obtain pure anthocyanin monomers, etc.

Active Publication Date: 2016-06-08
JIANGSU ACAD OF AGRI SCI
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, if the red meat peach anthocyanin is simply adsorbed and eluted with a macroporous resin of one nature according to the prior art or only re-purified by combining Sephadex, the anthocyanin with high purity cannot be obtained. monomer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating and purifying anthocyanins from red-fleshed peaches
  • Method for separating and purifying anthocyanins from red-fleshed peaches
  • Method for separating and purifying anthocyanins from red-fleshed peaches

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The present invention separates and purifies the method for anthocyanin from red flesh peach, and its steps comprise:

[0040] (1) Preparation of red-fleshed peach pulp material: take the fruit pulp of the 'Beijing Yixianhong' variety, grind it into powder with liquid nitrogen, and store it at -70°C for later use;

[0041] (2) Preparation of red-fleshed peach flesh anthocyanin crude extract A: use volume ratio ethanol: water: formic acid: trifluoroacetic acid = 70:27:2:1 mixed solution as extraction agent, get red-fleshed peach fruit Add 20g of the powder into 40ml of extraction agent, avoid light and ultrasonically extract, then centrifuge at 10,000rpm at 4°C for 10min, take the supernatant and concentrate it in vacuum at 30°C until there is no ethanol, and obtain the crude extract A;

[0042] (3) Preparation of the primary purified solution B of red-fleshed peach pulp anthocyanins: take the crude extract A and adsorb it with the activated macroporous resin AmberliteXA...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for separating and purifying anthocyanin from blood-flesh peaches. The method comprises steps as follows: blood-flesh peach fruit powder is subjected to extraction by a mixed solution as an extraction agent, the mixed solution is formed by ethyl alcohol, water, formic acid and trifluoroacetic acid at a volume ratio of 70:27:2:1, the mixture sequentially passes through macroporous resin Amberlite XAD-7, SepHadex LH-20 and macroporous resin SP700 for adsorption, elution and purification and is processed by a reversed-phase C18 chromatographic column after vacuum concentration, eluent containing dominant anthocyanin component in blood-flesh peach fruit is collected according to obtained chromatograms and is subjected to vacuum concentration and drying, and anthocyanin is obtained. According to the method, ethyl alcohol, water, formic acid and trifluoroacetic acid are adopted for extraction, and the mixture sequentially passes through the macroporous resin Amberlite XAD-7, the SepHadex LH-20 and the macroporous resin SP700 for adsorption and elution; a high performance liquid chromatography is utilized for representation, collection and concentration, and cyanidin-3-glucoside with purity up to 92.7% can be obtained; and with the adoption of the method, anthocyanin in the blood-flesh peach fruit can be effectively separated and extracted, an anthocyanin monomer with high purity is obtained, and requirements of various functional food, health care products and drug additives can be met better.

Description

technical field [0001] The invention relates to a method for separating and purifying anthocyanins from red-fleshed peaches, and belongs to the technical field of food processing. Background technique [0002] Red flesh peach is the most characteristic resource among peaches, and it is a key material for research, development and utilization in recent years. Red-fleshed peach flesh is dark red, and the dominant anthocyanin component is cyanidin 3-glucoside. [0003] On the one hand, anthocyanins endow horticultural products with gorgeous appearance quality, and flower color and fruit color are important components of commodity value; In addition, the strong pharmacological activity and antioxidant capacity of anthocyanins have been confirmed in many studies, and they are important medical resources for humans and animals. Therefore, it is of great significance to separate and prepare high-purity natural anthocyanins. [0004] Patent document CN104098633A discloses a metho...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88C07H17/065C07H1/08
Inventor 严娟沈志军蔡志翔杨勇郭绍雷马瑞娟俞明亮
Owner JIANGSU ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap