Breeding method of erect panicle, long-stigma and elongated upmost internode two-line male sterile lines
A technology with long panicle neck and long stigma is applied in the field of rice department and line breeding, which can solve problems such as environmental pollution and increase cost, and achieve the effects of reducing production cost, increasing economic benefit, and high outcrossing seed setting rate.
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Embodiment 1
[0020] 1) The erect panicle rice variety 259 (A), the long stigma rice variety Hai B (B), and the long panicle neck rice variety Longtepu eB1 (C) were selected as the parents for the target traits, and the two excellent lines with large promotion area in production were selected. The sterile line Guangzhan 63S is the recurrent parent (R), and it is used as a cross A / B to obtain a cross F 1 Generation, with F 1-AB express;
[0021] 2) Plant hybrid F 1 generation, crossed F at flowering stage 1-AB / C, have to repost F 1 , with F 1-ABC express;
[0022] 3) Planting F 1-ABC , F from the harvest 2-ABC , planting F 2-ABC , the group size is 2000 plants, the erect panicle, long stigma and long panicle neck traits are identified at the heading and flowering stage, and the plants with the erect panicle, long stigma and long panicle neck traits are screened and marked, and the seeds are harvested separately at the mature stage (F 3-ABC );
[0023] 4) Line planting 3) F obtain...
Embodiment 2
[0032] 1) The erect panicle rice variety 259 (A), the long stigma rice variety Hai B (B), and the long panicle neck rice variety Longtepu eB1 (C) were selected as the parents for the target traits, and the two excellent lines with large promotion area in production were selected. The sterile line Y58S is the recurrent parent (R), and it is used as a cross A / B to obtain a cross F 1 Generation, with F 1-AB express;
[0033] 2) Plant hybrid F 1 generation, crossed F at flowering stage 1-AB / C, have to repost F 1 , with F 1-ABC express;
[0034] 3) Planting F 1-ABC , F from the harvest 2-ABC , planting F 2-ABC , the population size is 2300 plants, the erect panicle, long stigma and long panicle neck traits are identified at the heading and flowering stage, and the plants with the erect panicle, long stigma and long panicle neck traits are screened and marked, and the seeds are harvested separately at the mature stage (F 3-ABC );
[0035] 4) Line planting 3) F obtained w...
Embodiment 3
[0044] 1) The erect panicle rice variety 259 (A), the long stigma rice variety Hai B (B), and the long panicle neck rice variety Longtepu eB1 (C) were selected as the parents for the target traits, and the two excellent lines with large promotion area in production were selected. The sterile line Pei'ai 64S is the recurrent parent (R), and it is used as a cross A / B to obtain a cross F 1 Generation, with F 1-AB express;
[0045] 2) Plant hybrid F 1 generation, crossed F at flowering stage 1-AB / C, have to repost F 1 , with F 1-ABC express;
[0046] 3) Planting F 1-ABC , F from the harvest 2-ABC , planting F 2-ABC , the population size is 2500 plants, the erect panicle, long stigma and long panicle neck traits are identified at the heading and flowering stage, and the plants with the erect panicle, long stigma and long panicle neck traits at the same time are screened and marked. 3-ABC );
[0047] 4) Line planting 3) F obtained with erect panicle, long stigma and long...
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