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A kind of preparation and debittering device of fructooligosaccharide

A technology for fructooligosaccharides and debitterness, which is applied in the preparation of oligosaccharides and sugar derivatives, biochemical cleaning equipment, etc., to achieve the effects of simple structure, solving bad taste and saving resources

Inactive Publication Date: 2016-11-30
深圳市华利康纤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no device suitable for the industrialization of the packed bed reaction of chicory-producing fructo-oligosaccharides on the market. Therefore, it is urgent to provide a production device that can be applied to chicory fructo-oligosaccharides

Method used

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  • A kind of preparation and debittering device of fructooligosaccharide
  • A kind of preparation and debittering device of fructooligosaccharide
  • A kind of preparation and debittering device of fructooligosaccharide

Examples

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preparation example Construction

[0035] The preparation and purification device of fructooligosaccharide provided by the present invention is composed of a first resin column, a second decolorization column, a third resin column, a fourth ultrafilter and a delivery pipeline connecting each device.

[0036] The first resin column is filled with anionic macroporous resin loaded with inulinase. The aspect ratio of the anionic macroporous resin filled is 9-15:1. The anionic macroporous resin is any one of D301, D301-SC, and D314. One is to immobilize inulinase on the surface of the resin by adsorption, and when the fructooligosaccharide feed liquid passes through the reactor, the inulinase can hydrolyze the β-2, l-glycosidic bond in the inulin. An insulating layer is arranged on the outside of the first resin column, and a temperature control device is arranged on the insulating layer, which can monitor the temperature of the first resin column and improve the enzyme activity efficiency of inulinase. The top of t...

Embodiment 1

[0044] Using the method in the examples, the inulinase was immobilized on the D301 resin, and the third resin was filled with LSD-001 macroporous resin.

[0045] Wash and peel 50kg of chicory, dissolve it in 25L of phosphate buffer solution with a pH of 7.2 and a concentration of 0.1mol / L and boil, then mash the chicory and stir thoroughly, heat for 15 minutes, filter to remove the chrysanthemum slag, collect filtrate. Adjust the temperature of the first resin column to 55°C, preheat the reactor, then put the chicory solution (with a temperature of about 55°C) into the first resin column from the feed liquid inlet, and wait until the feed liquid fills the first resin column, Let it stand for 1 hour, then return the feed liquid from the bottom of the first resin column to the first resin column, and let it stand for 1 hour. Take a sample from the sampling port to detect the type of sugar in the feed liquid. If the inulin in the feed liquid has been completely decomposed, open ...

Embodiment 2

[0048] (1) Resin pretreatment: use NaOH aqueous solution with a mass fraction of 50g / L to treat D301, D301-SC, and D314 resins from bottom to top at a flow rate of 3BV / h, wash with pure water until neutral, and then use 0.3mol / L of hydrochloric acid at a flow rate of 3BV / h to treat D301, D301-SC, and D314 resins from bottom to top, and then wash with water until neutral to obtain processed D301, D301-SC, and D314 resins.

[0049] (2) Immobilization of inulinase: mix the treated D301, D301-SC, D314 resin obtained in step (1) with inulinase in a ratio of 1kg: 1000U, and the ratio of resin to phosphate buffer is 1kg : Add phosphate buffer solution with a concentration of 0.1mol / L and a pH of 6.0 at a ratio of 1 L, stir and adsorb at 40°C for 3 hours, and then add glutaraldehyde to each system so that the final concentration of glutaraldehyde in the system is 0.1mol / L, react at 20°C for 3h, filter out the resin, and obtain macroporous resins D301, D301-SC, and D314 immobilized wi...

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Abstract

The invention discloses a device for preparing and debitterizing fructo-oligosaccharide. The device comprises a first resin column, a second decolorizing column, a third resin column, a fourth ultra-filter and conveying pipelines connected with each device. The device is characterized in that anionic macroporous resin immobilized with inulinase is filled in the first resin column, active carbon is filled in the second decolorizing column, non-polar macroporous resin is filled in the third resin column, and an ultrafiltration membrane is arranged in the fourth ultra-filter. The device disclosed by the invention has the advantages of simple operation, high debitterizing rate and the like, and has a relatively high industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of natural product processing equipment, and in particular relates to a device for preparing and debittering fructooligosaccharides. Background technique [0002] Fructose-oligosaccharides, also known as fructo-oligosaccharides, are kestose, kestose and kestrose formed by combining 1 to 3 fructosyl groups with fructosyl groups in sucrose through β(2-1) glycosidic bonds. A mixture of sugar and the like; about 60-70 grams of inulin in 100 grams of dry weight chicory, inulin is a fructan connected by a linear β-2,1-glycosidic chain, and its end is a sucrose group. Therefore, chicory powder is used as raw material to hydrolyze with endo-inulinase, and after refining, fructo-oligosaccharides can be finally obtained. [0003] Fructose-oligosaccharides have good health effects, are non-toxic, and are suitable for diabetic patients. They can prevent dental caries, regulate lipid metabolism, promote calcium absorpti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12P19/14C07H3/06C07H1/08
CPCC07H3/06C12M21/18C12M33/14C12P19/00C12P19/04C12P19/14
Inventor 黄振华杨云
Owner 深圳市华利康纤生物科技有限公司