Marine ammonia oxidizing archaea enrichment device with automatic control of dissolved oxygen
An automatic control and ammonia oxidizing archaeal technology, applied in biochemical cleaning devices, enzymology/microbiology devices, biochemical instruments, etc., can solve problems such as poor control of dissolved oxygen, loss of biomass, large hydraulic shear force, etc. Achieve the effect of increasing abundance, slowing growth and reducing loss
Active Publication Date: 2016-05-11
ZHEJIANG UNIV
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[0005] The purpose of the present invention is to overcome the shortcomings of existing culture devices such as poor control of dissolved oxygen, large hydraulic shear force, and serious loss of biomass, and provide a marine ammonia-oxidizing archaeal enrichment device with automatic control of dissolved oxygen
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[0011] Such as figure 1 As shown, the marine ammonia oxidizing archaeal enrichment device with automatic control of dissolved oxygen includes a reactor 1, a water inlet 2, a low-temperature water bath 3 and a control cabinet 4, wherein the reactor 1 includes a funnel-shaped tank body 5, and the reactor air pressure balance Port 6, reactor DO probe 7, reactor water outlet 8, reactor water inlet 9, temperature control jacket 10, temperature control jacket water outlet 11, temperature control jacket water inlet 12 and reactor water inlet pump 25, The water device 2 includes a first water inlet partition 13, a second water inlet partition 14, a third water inlet partition 15, a water distribution area 16, an aeration area 17, a sedimentation area 18, a drainage area 19, a water inlet DO probe 20, feeding pipe 21, feeding pump 22, feeding barrel 23, water inlet outlet 24, aeration disc 26, air pump 27, solenoid valve 28, nitrogen cylinder 29, water inlet pipe 30 , the water inlet ...
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The invention discloses a marine ammonia-oxidizing archaea enrichment device capable of automatically controlling dissolved oxygen. The enrichment device comprises a reactor, a water feeder, a low-temperature water-bath kettle and a control cabinet, wherein a main body of the reactor is a funnel-shaped tank body, a reactor pressure balance opening and a reactor DO probe are arranged at the upper end of the funnel-shaped tank body, a reactor water inlet is formed in the lower end of the funnel-shaped tank body, and a temperature-controllable jacket is arranged outside the funnel-shaped tank body; the water feeder is divided into four parts, namely, a water-distribution zone, an aeration zone, a sedimentation zone and a water drainage zone, the water-distribution zone is provided with a water feeder DO probe, a feeding pipe and a water feeder water outlet, the aeration zone is provided with an aerating disc, the sedimentation zone is provided with a water feeder water inlet pipe and the water drainage zone is provided with a water feeder gas outlet and a water feeder water drainage opening. The enrichment device can provide low-temperature, low-dissolved-oxygen, low-shearing-force and high-salinity environment required for the growth of marine ammonia-oxidizing archaea; by virtue of the dissolved oxygen probe, an air pump and a nitrogen steel cylinder, the dissolved oxygen in the reactor influent water is controlled at a relatively low level so that the effective enrichment of marine ammonia-oxidizing archaea is achieved and the high-purity enriched culture product is obtained.
Description
technical field [0001] The invention relates to a device for enriching marine ammonia-oxidizing archaea with automatic control of dissolved oxygen. Background technique [0002] Nitrification-denitrification biological denitrification is one of the most commonly used technologies for denitrification of wastewater, and the ammonia oxidation process is the key step and rate-limiting step in the nitrification process. For a long time, ammonia oxidizing bacteria have been considered as the main undertakers of the ammonia oxidation process, but recently, researchers have found that ammonia oxidizing archaea are also responsible for the ammonia oxidation process, because ammonia oxidizing archaea have unique physiological and biochemical characteristics and engineering application value , has received extensive attention. [0003] Ammonia oxidizing bacteria are common microorganisms in wastewater treatment systems, but are susceptible to environmental conditions, which limits the...
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IPC IPC(8): C12M1/04C02F3/34
Inventor 胡宝兰何崭飞刘帅叶天强徐新华田光明郑平
Owner ZHEJIANG UNIV