Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for stably producing porcine alpha interferon using recombinant Pichia pastoris

A technology of interferon alpha and Pichia pastoris, applied in the field of bioengineering, can solve the problems of accumulation of by-products of anaerobic metabolism, affecting the stability of fermentative production of interferon alpha in pigs, etc.

Active Publication Date: 2017-12-08
SHANGHAI ACAD OF AGRI SCI +1
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, the culture of Pichia pastoris is constantly switching back and forth between aerobic and anaerobic states, which easily causes the generation and accumulation of by-products of anaerobic metabolism, and ultimately seriously affects the normal induced expression of pig interferon alpha and the stability of fermentation production
[0004] So far, there are few reports on the generation and accumulation of metabolic byproducts in Pichia pastoris during high-density culture and their effects on the expression of target proteins during induction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for stably producing porcine alpha interferon using recombinant Pichia pastoris
  • A method for stably producing porcine alpha interferon using recombinant Pichia pastoris
  • A method for stably producing porcine alpha interferon using recombinant Pichia pastoris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The present inventor has found that pig interferon alpha cannot be expressed normally or fermentation performance is not good by studying whether and how the by-products of anaerobic metabolism accumulate when glycerol is fed, and the effect of accumulation of by-products of anaerobic metabolism on the induced expression of pig interferon alpha. The root cause of the stability, based on this, an improved DO-Stat glycerin feeding strategy was proposed. The method of the invention greatly improves the induced expression efficiency of the porcine alpha interferon and ensures the stability of fermentation.

[0024] In the present invention, "fermentation" refers to culturing the recombinant Pichia pastoris carrying the porcine interferon-alpha gene) to produce porcine-alpha interferon. More specifically, "fermentation" refers to a large-scale production process, such as a fermentation scale of 0.5-500 liters, such as a fermentation scale of 1 liter, 2 liters, 5 liters, 10 l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for stably producing porcine alpha interferon by using recombinant Pichia pastoris, and proposes an improved DO-Stat glycerin feeding strategy, the method of the present invention greatly improves the induced expression efficiency of porcine alpha interferon, The stability of fermentation is ensured.

Description

technical field [0001] The invention belongs to the technical field of bioengineering; more specifically, the invention relates to a method for stably producing porcine alpha interferon by using recombinant Pichia pastoris. Background technique [0002] Porcine α-interferon (porcine interferon-α, pIFN-α) is a veterinary drug with broad application prospects [Chinsangaram J et al., J Virol, 2001, 75(12):5498-5503]. Fermentation by recombinant Pichia pastoris is the main method for producing porcine interferon-alpha, which can be completely secreted in the fermentation broth. Based on the completion of the construction of recombinant bacteria and the optimization of the shake flask process, the research focus has shifted to the optimization of the fermenter-scale fermentation process. [0003] In recent years, studies on the optimization of porcine interferon-α induction process have shown that the high-efficiency induced expression of porcine interferon-α is directly related...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C12R1/84
Inventor 于瑞嵩史仲平李震高敏杰丁健董世娟朱于敏
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com