A detection method for copper ions based on a graphene oxide chip

Abstract

The invention discloses a method for detecting copper ions based on a graphene oxide chip, and belongs to the field of biomedicines. The method for detecting the copper ions based on the graphene oxide chip mainly comprises the following steps: (1) preparing a slide with a graphene oxide dot matrix; (2) connecting a Cu<2+> correspondingly cut DNAzyme sequence with the slide with the graphene oxide dot matrix; (3) detecting the cutting action of Cu<2+> on the slide. By virtue of the principle that fluorescence of a graphene oxide can be quenched by AuNP, single chain DNAzyme containing Cu<2+> cut sites can be cut off after the action of Cu<2+> and is dissociated from single chain DNAzyme which is fixed on the graphene oxide and one end is modified by amino and the other end is marked by AuNP, the distance between AuNP and the surface of the graphene oxide is changed, and the fluorescence of the graphene oxide is changed, and with the combination of a high-flux micro array chip technique, Cu<2+> can be detected with low cost and high sensitivity.

Description

technical field [0001] The invention belongs to a heavy metal detection method in the field of biomedicine, in particular to a graphene oxide biochip based on Cu 2+ detection method. Background technique [0002] Cu 2+ In the human body, it can strongly interact with proteins and various enzymes, making them inactive, and may also be enriched in some organs of the human body. If it exceeds the tolerance limit of the human body, it will cause acute poisoning, sub- Acute poisoning, chronic poisoning, etc., will cause great harm to the human body. Therefore, Cu 2+ Pollution has attracted people's attention in medicine, food, environment and other aspects. At present, there are more and more researches on copper ion detection methods, mainly including immunoassay, stripping voltammetry, test paper method, etc. Some existing methods lack higher sensitivity, and the detection throughput of samples is also low, so Establishing a system of highly sensitive and high-throughput d...

AI Technical Summary

Problems solved by technology

At present, there are more and more researches on copper ion detection methods, mainly including immunoassay, stripping voltammetry, test paper method, etc. Some existing methods lack higher sensitivity, and the detection throughput of samples is also low, so Establishing a high-sensitivity, high-throughput detection method system is an urgent research content that needs to be developed at present.

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