Detection method of pharmaceutical composition Gelan Xinning for treating coronary heart disease and angina
A detection method and composition technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of gypenoside without formulating content determination control methods, etc.
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Embodiment 1
[0102] Example 1 A medicinal composition for treating coronary heart disease and angina pectoris (Glanxinning soft capsule)
[0103] 1. Prescription: Pueraria lobata total flavonoids 200g, hawthorn extract 60g, gypenosides 20g, salad oil 254g, beeswax 6g, hydrogenated palm oil 20g, soybean phospholipids 10g, methyl silicone oil 0.5g, made into 1000 capsules.
[0104] 2. Preparation method: Take salad oil, add beeswax, hydrogenated palm oil, heat to dissolve, let cool, add soybean phospholipid, stir well, add Pueraria lobata total flavonoids, hawthorn extract, Gynostemma pentaphyllum total glycosides, stir well, colloid mill and grind well , Add methyl silicone oil, decompress and defoam, make a soft capsule with liquid medicine, and get it.
Embodiment 2
[0105] Example 2 Establishment of Methodology for Determination of Gypenoside XLIX by Liquid Chromatography
[0106] 1. Solution preparation and determination:
[0107] Preparation of reference solution
[0108] Accurately weigh an appropriate amount of Gypenoside XL IX reference substance dried under reduced pressure at 80°C for 3 hours, accurately weigh it, and add methanol to make a solution containing 0.5 mg per 1 ml.
[0109] Preparation of test solution
[0110] Take the contents of 10 soft capsules prepared in Example 1 (try to take as little as possible the remaining inside of the capsule), mix the contents, take about 1.5g, put it in a conical flask, add 40ml of 10% methanol, and reflux in a water bath for 40 minutes. Filter with a small amount of cotton, wash the glass container and cotton with 10ml of water, combine the filtrate in a separatory funnel, shake and extract with 40ml of ether, discard the ether solution, shake and extract the water solution 3 times with water-s...
Embodiment 3
[0176] Example 3 Identification of Puerarin by Thin Layer Chromatography
[0177] 1. Test conditions: Double tank expansion cylinder (Shanghai Xinyi); Silica gel G (for thin layer chromatography, Qingdao Ocean Chemical Factory); Sodium carboxymethyl cellulose (Sinopharm Group Chemical Reagent Co., Ltd.); self-made thin layer plate, thickness 0.3mm. The remaining reagents are of analytical grade.
[0178] 2. Test procedure: Take 0.6 g of the contents of the soft capsule prepared in Example 1 and place it in an Erlenmeyer flask, add 10 ml of methanol, sonicate for 10 minutes, stand still, filter, and use the filtrate as the test solution.
[0179] Take puerarin reference substance, add methanol to make a solution containing 1mg per 1ml, as the reference substance solution.
[0180] According to thin layer chromatography ("Chinese Pharmacopoeia" 2010 edition one appendix VI B) test, draw 2 ~ 4μl of each of the above two solutions, respectively spot on the same silica gel G thin layer p...
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