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A kind of method for extracting flavonoids active ingredient in Chinese medicine Citrus aurantium

A technology of active ingredients and flavonoids, applied in the field of extraction and detection of natural medicines, to achieve high extraction efficiency and good extraction and detection results

Active Publication Date: 2017-04-19
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Up to now, there has been no report on the extraction and detection of active ingredients of traditional Chinese medicine using ionic liquids as eluents, combined with matrix solid-phase dispersion extraction technology.

Method used

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  • A kind of method for extracting flavonoids active ingredient in Chinese medicine Citrus aurantium
  • A kind of method for extracting flavonoids active ingredient in Chinese medicine Citrus aurantium
  • A kind of method for extracting flavonoids active ingredient in Chinese medicine Citrus aurantium

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Experimental program
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Effect test

preparation example Construction

[0046] The specific steps of the preparation method of the Fructus Fructus reference substance solution are as follows: respectively take appropriate amounts of reference substances of neohesperidin and naringin, weigh them accurately, mix them in a brown measuring bottle, add methanol to make 500 μg of neohesperidin per 1 mL , A solution of 500 μg of naringin was obtained as a reference substance solution.

[0047] The ionic liquid bromide 1-ethyl-3-methylimidazole used in the embodiment of the present invention, 1-butyl-3-methylimidazole methanesulfonate, 1-hexyl-3-methylimidazole bromide, 1 -Butyl-3-methylimidazolium tetrafluoroborate and 1-hexylpyridine tetrafluoroborate were purchased from Alfa Aesar.

Embodiment 1

[0049] Weigh 150 mg of florisil and 50 mg of Fructus aurantii herb powder, add them into a mortar and grind for 1 min. Add the uniformly mixed solid mixture into five 3mL solid-phase extraction cartridges respectively, add a sieve plate to the bottom of the cartridge in advance, and then add a sieve plate to the top of the cartridge after the addition is complete. Place all filled cartridges on the solid phase extraction apparatus. Measure 0.4mL of different types of ionic liquid aqueous solutions, the ionic liquids are 1-ethyl-3-methylimidazole bromide, 1-butyl-3-methylimidazolium methanesulfonate, 1-hexyl-3-bromide -Methylimidazole, 1-butyl-3-methylimidazole tetrafluoroborate, 1-hexylpyridine tetrafluoroborate, the concentration of the above five ionic liquid aqueous solutions is 250mM, injected into the small column, start solidification The aspirating pump of the phase extractor (the air pressure is -28.0 bar) is used to elute the small column. The eluate was collected i...

Embodiment 2

[0057] Weigh 150 mg of florisil and 50 mg of Fructus aurantii herbal powder, and add them into a mortar and grind for 1 min. Add the well-mixed solid mixture to four 3mL SPE cartridges respectively, add a sieve plate to the bottom of the cartridge in advance, and then add a sieve plate to the top of the cartridge after the addition is complete. Place all filled cartridges on the solid phase extraction apparatus. Measure 0.4mL of 1-butyl-3-methylimidazolium tetrafluoroborate aqueous solution with different concentrations (150mM, 200mM, 250mM, 300mM), inject it into the small column, and start the aspirating pump of the solid phase extraction instrument (the air pressure is - 28.0bar), the column was eluted. The eluate was collected in 1.5mL centrifuge tubes respectively. After the elution, the liquid in the centrifuge tube was vortexed for 10s, then put into the centrifuge, and centrifuged at 13000rpm for 5min. The supernatant was collected as a sample, and analyzed by ultra...

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Abstract

The invention discloses a method for extracting a flavonoid active ingredient from traditional Chinese medicine fructus aurantii. The method comprises the following steps: taking fructus aurantii medicine powder and florisil, mixing in a mass ratio of 1: (1 to 4), adding the fructus aurantii medicine powder and florisil in a mortar and grinding; taking a solid-phase extraction column, adding a sieve plate at the bottom of the solid-phase extraction column, adding the ground solid mixture into the solid-phase extraction column, compactly filling a filler and then adding a sieve plate at the top, placing the filled solid-phase extraction column on a solid-phase extractor, taking an eluent, injecting the eluent into the solid-phase extraction column, opening an air pump of the solid-phase extractor, eluting the solid-phase extraction column, collecting elution solution; by the end of the elution, whirling and centrifuging the elution solution, and taking the supernatant, that is, flavonoid active ingredient extraction solution, wherein the eluent is ionic liquid aqueous solution with a concentration of 150-300mmol / L. According to the method disclosed by the invention, an ionic liquid is used as the eluent and a matrix solid-phase dispersive extraction technology is combined, so that the flavonoid active ingredient in the fructus aurantii medicinal material can be extracted with high efficiency in an environmentally friendly way.

Description

technical field [0001] The invention belongs to the field of extraction and detection of natural medicines. It relates to a micro-extraction method of traditional Chinese medicine, specifically, it relates to a new method for extracting flavonoid active ingredients in the traditional Chinese medicine Fructus Aurantii by using ionic liquid as eluent and combining micro-matrix solid-phase dispersion extraction technology. Background technique [0002] Fructus aurantii (Fructus aurantii) is the dried young fruit of Rutaceae plant lime (Citrus aurantium L.) and its cultivars or sweet orange (Citrus sinensis Osbeck). Warm in nature, bitter, pungent, sour in taste, slightly cold in nature, returns to spleen and stomach meridian. It has the effect of regulating qi, broadening the middle, moving stagnation and reducing swelling. It is mainly used to treat sternocostal qi stagnation, fullness and pain, indigestion, stagnation of phlegm and retention, gastroptosis, prolapse of the an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/752G01N30/02
Inventor 曹君胡帅帅曹婉徐菁菁代菡镔达建华
Owner HANGZHOU NORMAL UNIVERSITY
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