Biocontrol strain ZHR0 for prevention and control of sugarcane smut and application thereof

A technology of smut and biocontrol fungicide, applied to the biocontrol strain ZHR0 for the prevention and treatment of sugarcane smut and its application field, which can solve environmental pollution, difficult to overcome disease resistance and yield and quality traits, difficult to control chemical drugs, etc. problem, to achieve good control effect

Active Publication Date: 2015-05-06
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to screen high resistance genes by traditional breeding methods, and it is difficult to overcome the contradiction between disease resistance and yield and quality traits; it is difficult to control systemic diseases with chemical drugs, and chemical control bacteria are prone to drug resistance, causing environmental pollution

Method used

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  • Biocontrol strain ZHR0 for prevention and control of sugarcane smut and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The Bacillus amyloliquefaciens ZH0 (Bacillus amyloliquefaciens ZH0) strain is a strain of Bacillus amyloliquefaciens isolated from sugarcane leaves in September 2013.

[0018] A kind of screening method of bacterial strain, described bacterial strain is Bacillus amyloliquefaciens ZHRO (bacillus amyloliquefaciens ZHRO) bacterial strain, and the method comprises the following steps:

[0019] (1) Take several sugarcane leaves and put them into a sterilized plastic bag and bring them back to the laboratory quickly. Take 5g of sugarcane leaves and cut them into pieces after being sterilized and put them into a sterilized triangular flask, add 50ml of sterile water, and sterilize Glass beads 5g, shake on a shaker at 150-180rpm for 30min, then let stand for 10min;

[0020] (2) Draw the supernatant and dilute to 10 -4 、10 -5 、10 -6 For three gradients, draw 0.1mL liquid from each of the three gradient dilutions and inoculate them on LB solid medium, culture at a constant tem...

Embodiment 2

[0025] Described Bacillus amyloliquefaciens ZHR0 (bacillus amyloliquefaciens ZHR0) and the first group of confrontation culture test of sugarcane smut, concrete method is as follows:

[0026] First draw two mutually perpendicular straight lines at the center of the plate bottom containing the PDA medium, draw 5ul of the described Bacillus amyloliquefaciens ZHR0 (bacillus amyloliquefaciens ZHR0) bacterial strain cultivated overnight on the LB liquid medium with a sterilized gun tip to inoculate in plate center; draw 5ul sugarcane smut bacteria solution (1X10 6 CFU / ml, positive burden spores 1:1) were dropped along two vertical lines at a distance of 2 cm from the center, and placed in an incubator at 28°C for 3 days. The colony in the center of the plate is the Bacillus amyloliquefaciens ZH0 (Bacillus amyloliquefaciens ZH0) strain, surrounded by four colonies of S. amyloliquefaciens.

[0027] It can be seen that the growth of smut is significantly inhibited near the ZHR0 side ...

Embodiment 3

[0030] The second group confrontation culture test of described Bacillus amyloliquefaciens ZHR0 (bacillus amyloliquefaciens ZHR0) and sugarcane smut, concrete method is as follows:

[0031] (1) will be divided into 1, 2, 3 and 4 four regions equally on the plate containing PDA medium;

[0032] (2) The first test group: draw two mutually perpendicular straight lines at the center of area 1, draw 5ul of the described Bacillus amyloliquefaciens ZHR0 (Bacillus amyloliquefaciens ZHR0) bacterial strain cultivated overnight on the LB liquid medium with a sterilized pipette tip Inoculate at the intersection of two mutually perpendicular straight lines; draw 5ul sugarcane smut bacteria liquid (1X10 6 CFU / ml, positive burden spores 1:1) drop along two vertical lines at a distance of 1cm from the intersection point;

[0033] (3) The first control group: draw two mutually perpendicular straight lines at the center of the area 3, inoculate 5ul sterile water at the intersection of the two ...

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Abstract

The invention discloses a strain, and the strain is a Bacillus amyloliquefaciens ZHR0 strain collected in the China Center for Type Culture Collection (CCTCC) with a collection number of CCTCC NO:M 2014577. The invention further discloses a preparation method of ZHR0 biocontrol microbial inoculum. Experiments show that the control efficiency of the ZHR0 biocontrol microbial inoculum prepared by the preparation method to the sugarcane smut is up to 53.98%, which indicates that the Bacillus amyloliquefaciens ZHR0 strain has good prevention and control effects on the sugarcane smut.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a biocontrol bacterial strain ZH0 for preventing and treating sugarcane smut and application thereof. Background technique [0002] Sugarcane smut (sugarcane smut) is a fungal disease caused by Ustiliago scitaminea Sydow. It was first reported in Natal, South Africa in 1877, and the disease has become an important worldwide epidemic disease. Infected cane seedlings lead to early germination, increased tillers, narrow, light green leaves, and small cane stems. The typical symptom of sugarcane smut is to produce a long unbranched black whip (called black spike whip) at the tip of the diseased sugarcane, with different lengths, short straight or slightly curved, and long curly downward. The center of the black spike whip is a stem composed of parenchyma or vascular tissue, and a layer of black teliospores is attached to the outside of the stem. After the telios...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 张木清徐世强王继华
Owner GUANGXI UNIV
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