Method for preparing White (carbon-source-containing) solid culture medium in large scale
A technology of a solid culture medium and a production method, which is applied in the field of plant biology and can solve the problems of precipitation, mother liquor infecting bacteria, molds, and easy calculation errors.
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[0012] The present invention will be further described below in conjunction with specific embodiments.
[0013] In this embodiment, a large-scale production method of White (containing carbon source) solid culture substrate includes five steps of accurate calculation, accurate weighing, first mixing less and then more, sealing, and unsealing for use.
[0014] The specific operation of the above five steps is as follows:
[0015] ① Accurate calculation: expand the 16 elements contained in the White (containing carbon source) solid medium by 1000 times, that is, pyridoxine hydrochloride 0.1g, thiamine hydrochloride 0.1g, nicotinic acid 0.3g, potassium iodide 0.75g, boric acid 1.5 g, 2.5g iron sulfate, 3g glycine, 3g zinc sulfate heptahydrate, 7g manganese sulfate tetrahydrate, 16.5g sodium dihydrogen phosphate, 65g potassium chloride, 80g potassium nitrate, 200g sodium sulfate, 200g calcium nitrate tetrahydrate, magnesium sulfate 720g, soft white sugar 30000g;
[0016] ② Accur...
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