Method for identifying hybrids of Brassica napus and Brassica napus

Abstract

The invention relates to a method for identifying hybrid seeds of winter cole-wort and mustard type winter rape. With the adoption of the method, the condition that the hybrid seeds of winter cole-wort and mustard type winter rape are real hybrid seeds is identified. The method for identifying the hybrid seeds of winter cole-wort and mustard type winter rape is characterized by comprising steps as follows: (1), sampling; (2), weighing 0.2-0.3 g of flower buds, flowers and silique respectively, performing homogenizing by adopting a phosphate buffer having the pH of 7.8 and containing 2 mmol of ASA (acrylonitrile styrene acrylate) and 5 mmol of EDTA-Na2 (ethylene diamine tetraacetic acid-Na2) under ice-bath respectively, performing centrifugation for 10 min at 12,000 r / min, and taking a liquid supernatant and storing the liquid supernatant in a refrigerator at the temperature of subzero 20 DEG C for standby. Polyacrylamide gel electrophoresis is adopted, concentrations of separation gel and spacer gel are 7% and 4% respectively, and pH values are 8.8 and 6.8 respectively; an electrode buffer adopts a Tris-glycine system, the pH value is 8.3, and the loading quantity of samples is 30 mu L; when electrophoresis starts, steady voltage is 100 V, the voltage is regulated to be 200 V after the samples pass the concentrated gel, and the electrophoresis time is about 5 h; after electrophoresis is finished, the samples are placed in a dyeing liquid benzidine solution for dyeing until a clear blue stripe can be observed, and the samples are photographed for storage after washed by flowing water; a migration rate Rf value is quotient obtained by dividing the migration distance from an original point to the stripe by the distance from the original point to an indicator.

Description

technical field

[0001] The invention relates to a method for identifying hybrids of Brassica napus and Brassica napus hybrids. Using this method, the hybrids of Brassica napus and Brassica napus were successfully identified as true hybrids. Background technique

[0002] Distant hybridization refers to the hybridization between different species, genus or species with more distant relationship, including interspecific hybridization, intergeneric hybridization and inter-subspecies hybridization. Distant hybridization is an important means of breeding and an important way to create new types of plants and new varieties of crops. Through distant hybridization, the boundaries between species and genus can be broken, the exchange or combination of genetic material between different species can be made possible, and plant traits can be improved purposefully to form new species and varieties with stronger vitality. According to whether the newly formed species completely contain t...

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