A kind of preparation method of a, c-type Clostridium perfringens toxoid and bacterium bivalent two-type antigen vaccine of chicken necrotic enteritis

Abstract

The invention relates to the field of animal bacteriology and provides a method for preparing two types of bivalent antigen vaccines of A, C type clostridium perfringens toxoid and thallus of necrotic enteritis. The method comprises the following steps: inoculating A type and C type clostridium perfringens enrichment fluid in a toxin-producing culture medium, and shake cultivating in an anaerobic environment of 43 DEG C for 5 hours to produce toxin; and inactivating via formaldehyde, adding an adjuvant to emulsify to obtain two types of bivalent antigen vaccines of A, C type clostridium perfringens toxoid and thallus. The protection rate of vaccine immune commercial chickens obtained by the method reaches 100% after counteracting toxic substances, which proves that the toxoid and thallus antigen complex vaccine can generate a good immune protection effect on pathogenic infection of bacteria and pathogenicity of bacterial toxins on poultry.

Description

technical field [0001] The invention relates to the field of animal bacteriology. The invention provides a preparation method of type A and type C Clostridium perfringens toxoid and bacterium bivalent two-type antigen vaccine for chicken necrotic enteritis. Background technique [0002] Necrotic enteritis (NE) is mainly caused by type A or type C Clostridium perfringens, but type C is not common. Intestinal mucosal necrosis and other characteristic lesions are produced, and dead birds are characterized by hepatomegaly, intestinal bleeding and intestinal mucosal bran-like necrosis. The disease can occur in a variety of poultry, and necrotic enteritis has been confirmed to occur in broilers, layers, turkeys, and quails. With the prohibition of antibiotic growth promoters, the disease has occurred in major poultry-raising countries and regions all over the world, and the global economic loss has reached 2 billion US dollars. [0003] Clostridium perfringens is an anaerobic, s...

AI Technical Summary

Problems solved by technology

In 2011, Saleh et al prepared Type A, Type C and Type A+C toxoids by using local prevailing strains, subcutaneously injected 7-day-old commercial broiler chickens, 0.5ml / bird, and exempted once at 21-day-old with the same dose and route. At the age of 35 days, mixed feeding (2L Clostridium perfringens broth culture + 1kg feed or 1L Clostridium perfringens broth culture + 2L water) challenged the virus, and the results proved that immune chickens, especially immune A+ Intestinal damage of chickens with type C toxoid is significantly reduced, but the above method uses toxoid to immunize broiler chickens directly, which has many limitations, such as short immunization period and the need for multiple booster immunizations.