Salmonella arizona agar medium and use thereof

A technology of agar medium and culture medium, which is applied in the field of microorganisms and achieves the effects of simple formulation, low variation rate, and favorable popularization and application.

Inactive Publication Date: 2015-05-27
刘汉斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except typhoid bacillus, paratyphoid A and paratyphoid bacillus causing human diseases, most of them can only cause animal diseases such as livestock, rodents and poultry, but sometimes they can also contaminate human food and cause food poisoning

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0022] A kind of Arizona bacterium agar medium, the component of described medium comprises 30.5g / L GN enrichment liquid, 12.0g / L sucrose, 10.0g / L bovine bile salt, 5.58g / L sodium thiosulfate, 4.5g / L sodium malonate, 22.0g / L dulcitol, 1.5g / L ferric ammonium citrate, 0.023g / L phenol red, 16.5g / L agar, the pH value is 7.0-7.2.

[0023] On the basis of the above scheme, the GN enrichment solution contains tryptone 20.0g, glucose 2.0g, dipotassium hydrogen phosphate 1.5g, mannitol 3g, sodium citrate 5.8g, Sodium Oxycholate 0.33g, Sodium Chloride 5.0g, Dipotassium Hydrogen Phosphate 2.0g.

[0024] The invention also discloses the application of the Arizona bacterium agar medium for selective isolation of Arizona Salmonella.

[0025] Peptone provides nitrogen source, vitamins, and growth factors; sodium chloride maintains a balanced osmotic pressure; dulcitol, sucrose, and glucose are fermentable sugars, and the colonies that ferment sugar to produce acid are yellow; Ar...

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PUM

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Abstract

The invention relates to the field of microbes and especially relates to a salmonella arizona agar medium. The salmonella arizona agar medium comprises 30.5g / L of GN enrichment broth, 12.0g / L of cane sugar, 10.0g / L of bile salt from ox, 5.58g / L of sodium thiosulfate, 4.5g / L of sodium malonate, 22.0g / L of dulcitol, 1.5g / L of ammonium ferric citrate, 0.023g / L of phenol red, and 16.5g / L of agar. The salmonella arizona agar medium has a pH value of 7.0-7.2. Each liter of the GN enrichment broth comprises 20.0g of tryptone, 2.0g of glucose, 1.5g of dipotassium hydrogen phosphate, 3g of mannitol, 5.8g of sodium citrate, 0.33g of deoxysodium cholate, 5.0g of common salt and 2.0g of dipotassium hydrogen phosphate. The salmonella arizona agar medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.

Description

Technical field [0001] The invention is a microorganism, which involves a kind of Arizona agar culture and its application. Background technique [0002] The pathogen of Salmon Cycidia.It belongs to the Department of Enterobacter, Gram -negative Enterobacter.Nearly a thousand (or strains) were discovered.According to its antigen component, the Coccusions are divided into basic fungus groups such as A, B, C, Ding, and Patrol.Among them, the main diseases related to human diseases include the Pullet in Group A, the Pullet Byphrobacteria Bacteria and Rat Typhoid in Group B, the Parallel Cycles of the Parallel Cycles and the Pyranophytics of the P. of the Typhoid Typhoid and enterulobacterium.Except for typhoid bacteria, paramilidae, and Permanent Typhoid Bybalobacteria, most of them can only cause diseases such as animal, rats and poultry, but sometimes it can also pollute human food and cause food poisoning.Salmonella is not easy to reproduce in the water, but it can survive for 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/42
CPCC12N1/20
Inventor 刘汉斌
Owner 刘汉斌
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