Method for seed reproduction of huge multi-leaf tobacco variety
A technology for breeding seeds and tobacco, applied in fertilization methods, horticultural methods, botanical equipment and methods, etc., can solve problems such as difficulties in breeding giant multi-leaf varieties, and achieve the effects of shortening the breeding period, easy management, and quality assurance
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Embodiment 1
[0014] In late July 2013, a multi-leaf single plant was found in the K326 breeding field of the Hubei Provincial Tobacco Research Institute Breeding Center in Fangxian County, Hubei Province, with 38 leaves that had not yet budded, while other surrounding tobacco plants had blossomed and borne fruit. Except that the leaves of this tobacco plant are smaller than other tobacco plants of K326, the plant type and leaf characteristics are very similar to those of K326, and there is no disease in the field. So it was marked, and after flowering, it was bagged separately and reserved for seeds. However, due to the worker's misoperation, the tobacco plant was topped, leaving 22 leaves. So use a cotton ball and a brush to dip the compound solution containing 6-BA 20 mg / L and NAA 5 mg / L that has been prepared during K326 propagation and apply it on the 2nd to 5th leaf axils downward from the top of the main stem of the tobacco plant. . At the same time, apply 2 Kg of pure nitrogen, N:...
Embodiment 2
[0016] In February 2014, the seeds of the multi-leaf tobacco strain harvested in Example 1 were sown in the Lichuan Breeding Base of the Hubei Academy of Tobacco Science, and the emergence was normal. On May 5, 30 plants were planted in the germplasm resource nursery using conventional cultivation measures. , In late July, more than 30 leaves had grown and buds had not yet appeared, so 10 plants with better growth were selected for topping, and 18 leaves were left, and the seeds were planned to be reserved through the branch tobacco. The rest of the individual plants were not topped and kept as observation records. On the day of topping, use the pre-prepared compound solution containing 100 mg / L of 6-BA and 10 mg / L of NAA to evenly spray the leaves and stems within 1-6 leaves from the top of the main stem of the topping tobacco plants and leaf axils. On the second day of topping, apply 1 Kg of pure nitrogen and N:P per mu 2 o 5 :K 2 O=1:2.0:2.5 calculation, topdressing tob...
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