Preparation and application of paper-based microfluidic chip for colorimetric analysis

A microfluidic chip, colorimetric analysis technology, applied in laboratory containers, analysis by chemical reaction of materials, material analysis by observing the influence of chemical indicators, etc., can solve the trouble of detection operation, Contamination, contamination of flowing liquid, etc., to achieve the effects of low production cost, strong operability, and short production cycle

Inactive Publication Date: 2015-06-03
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former will cause the liquid to penetrate longitudinally in the hydrophilic channel to the back of the channel, causing the flowing liquid to be polluted and the objects in contact with it; while the latter has only a hydrophilic channel and no substrate, so an additional clamping device is required when using it. Bring some trouble to the detection operation

Method used

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  • Preparation and application of paper-based microfluidic chip for colorimetric analysis
  • Preparation and application of paper-based microfluidic chip for colorimetric analysis
  • Preparation and application of paper-based microfluidic chip for colorimetric analysis

Examples

Experimental program
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specific Embodiment approach 1

[0044] as attached figure 1 , attached figure 2 And attached image 3 As mentioned above, the method for preparing a paper-based microfluidic chip for colorimetric analysis provided by the present invention is implemented. The prepared paper-based microfluidic chip is made of hydrophilic channels bonded on hydrophobic channels by spraying glue. formed on a substrate, or pasted together by any other means, where:

[0045] The preparation method of described hydrophobic base is carried out according to the following steps:

[0046] First, at room temperature, dissolve methyltrichlorosilane and octadecyltrichlorosilane in n-hexane solution at a volume ratio of 7:3, stir and mix evenly, and the concentration of the mixed solution is 0.1%;

[0047] Secondly, at room temperature, place the filter paper in the above mixed solution and soak for 5 minutes;

[0048] Finally, take out the soaked filter paper, put it in a constant temperature drying oven, and heat and dry it for 5 mi...

Embodiment 1

[0056] A method for preparing a hydrophobized substrate of a paper-based microfluidic chip for detecting the concentration of hexavalent chromium in water includes the following steps.

[0057] (1) Take 7 μL and 3 μL of methyltrichlorosilane and octadecyltrichlorosilane from Sigma Company and 10 mL of n-hexane solution from Aladdin Company and mix evenly at room temperature.

[0058] (2) Soak a rounded triangle filter paper with a side length of 6cm in the above hydrophobizing solution for 5 minutes at room temperature, take it out, put it in a clean constant temperature drying oven at 40°C, and heat and dry it for 5 minutes to obtain a hydrophobizing substrate.

[0059] (3) Design the shape of the hydrophilic channel with CorelDRAW software, then print it on the filter paper, and finally cut out the hydrophilic channel with scissors according to the printed pattern.

[0060] (4) Weigh 0.06g and 0.3g of 1,5-diphenylcarbazide and methyltrioctylammonium chloride respectively, di...

Embodiment 2

[0064] The application of the paper-based microfluidic chip prepared in Example 1 for detecting hexavalent chromium in water includes the following steps:

[0065] (1) use K 2 CrO 4 Prepare 1000mg / L hexavalent chromium solution and adjust to pH=4 with HCL and NaOH solution. Then diluted to 100mg / L, 25mg / L, 10mg / L, 5mg / L, 2mg / L, 0.5mg / L respectively.

[0066] (2) Use a pipette gun to pipette 2 μL of samples of different concentrations and drop them on the detection area, and completely react with the chromogenic agent flowing to the detection area through the microchannel.

[0067] (3) Repeat the experiment, scan the color development results with a scanner, use Photoshop software to obtain the M values ​​of different color development areas in CMYK color mode, and finally import the obtained data into Origin for fitting, and obtain the color development agent at a certain concentration The lower color intensity curve varies with the concentration of hexavalent chromium.

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Abstract

The invention relates to preparation and application of a paper-based microfluidic chip for colorimetric analysis; the preparation comprises the step of enabling a hydrophilic channel to be jointed on a hydrophobic substrate, wherein the hydrophilic channel is obtained by printing the pattern of the channel on a piece of filter paper and then shearing the pattern, and the hydrophobic substrate is obtained by treating the filter paper with a normal hexane solution containing methyl trichlorosilane and octadecyl trichlorosilane; the paper-based microfluidic chip is combined with a colorimetric analysis method, so that rapid quantitative detection can be realized. The preparation method is simple and fast, short in production cycle, low in cost, low in energy consumption and free from pollution. The preparation and the application of the paper-based microfluidic chip for colorimetric analysis are suitable for the fields such as clinical diagnosis, food hygiene, environmental monitoring and biochemistry, thus having very wide application scope.

Description

technical field [0001] The invention relates to a manufacturing method and application of a paper-based microfluidic chip, in particular to a method for preparing a paper-based microfluidic chip for colorimetric analysis. Background technique [0002] As an emerging microfluidic analysis technology platform, the microfluidic chip not only significantly reduces the consumption of reagents, but also effectively improves the analysis efficiency. Therefore, it has great application prospects in the fields of clinical diagnosis, food hygiene, environmental monitoring and biochemistry. [0003] The preparation of traditional microfluidic chips based on silicon materials, quartz and glass materials, and polymers is not only complicated in operation, high in cost, but also requires strict process conditions, which limits the promotion and application of microfluidic chips. Since the Whitesides group first proposed the concept of paper-based microfluidic chips (microfluidic paper-ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78B01L3/00
Inventor 李晓春王建花于化忠
Owner TAIYUAN UNIV OF TECH
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